Cytokeratin 7 Antibody (OV-TL 12/30)

Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL 12/30) [NBP1-22539] - Formalin fixed paraffin embedded human lung stained with Cytokeratin 7 antibody (NBP1-22539).

Product Discontinued

Cytokeratin 7 Antibody (OV-TL 12/30) Summary

• Immunogen: BALB/C mice were immunized with ovarian carcinoma cell line, OTN11.
• Localization: Cytoplasmic
• Specificity: This antibody recognizes keratin polypeptide of 54 kD. It stains a number of epithelial cells including ductal and glandular epithelia. Stratified squamous epithelia are positive but transitional epithelium of the urinary tract does not show any staining. This antibody labels adenocarcinomas and transitional carcinomas but does not react with squamous cell carcinomas.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT7
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS (pH 7.4) and 0.2% BSA
• Preservative: 0.09% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for Cytokeratin 7 Antibody (OV-TL 12/30)

• CK7
• CK-7
• Cytokeratin 7
• cytokeratin-7
• K2C7
• K7
• K7keratin, 55K type II cytoskeletal
• keratin 7
• keratin, type II cytoskeletal 7
• keratin-7
• MGC129731
• MGC3625
• sarcolectin
• SCLkeratin, simple epithelial type I, K7
• type II mesothelial keratin K7
• Type-II keratin Kb7

Background

Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 7.8. The individual human cytokeratins are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. Cytokeratin 7 is a 54kD intermediate filament protein found in a variety of glandularepithelia. Cytokeratin 7 has been found in columnar and glandular epithelium of the lung, cervix, breast, bile ducts and larger collecting ducts of the kidney. It is present in the transitional epithelium of bladder as well as ovarian and lung epithelia, and occasionally staining of blood vessel cell walls, particularly endothelial cells, may be observed. However, Cytokeratin 7 is not expressed by epithelial cells of the gastrointestinal tract, colon or prostate. Keratin 7 is often co expressed with keratin 19.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cytokeratin 7 Antibody (NBP1-22539)(2)

Publications using NBP1-22539

• Dariane, C;Clairefond, S;Peant, B;Communal, L; High Keratin-7 Expression in Benign Peri-Tumoral Prostatic Glands Is Predictive of Bone Metastasis Onset and Prostate Cancer-Specific Mortality Cancers [PMID: 35406395] (WB, Human)
• Duan L, Schimmelmann M, Wu Y et al. CCN3 Signaling Is Differently Regulated in Placental Diseases Preeclampsia and Abnormally Invasive Placenta Frontiers in endocrinology 2020-11-16 [PMID: 33304321] (IHC-P, Human)

FAQs for Cytokeratin 7 Antibody (NBP1-22539). (Showing 1 - 1 of 1 FAQs).

1. Would you let us know the concentration of #NBP1-22539?
   • Lot: E373 (current lot): 0.49ug/ml

Bioinformatics

• Gene Symbol: KRT7