DAPI Solution

Immunocytochemistry/Immunofluorescence: DAPI Solution [NBP2-31156] - Staining image showing the use of DAPI solution (Blue) [NBP2-31156] as a counterstaining agent in ICC/IF analysis of 58K Golgi Protein and alpha tubulin in Hela cell line. Cells, cultured on cover slips, were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with 1:200 dilution of anti-58K Golgi Protein antibody clone 58K-9 for overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) secondary at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI solution (Blue) and the cells were then imaged using a 40X objective.

Immunohistochemistry: DAPI Solution [NBP2-31156] - DAPI labelling in mouse brain. Image from verified customer review.

Immunocytochemistry/Immunofluorescence: DAPI Solution [NBP2-31156] - ICC analysis of LM7 cells using anti-IgM antibody [DyLight 550] (Cat.# NBP2-34422R). (blue) was used as a nuclear counterstain. Image from verified customer review.

• Reactivity: Hu
• Applications: Flow, ICC/IF, IHC, IHC
• Concentration: Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

DAPI Solution Summary

• Description: DAPI is excluded by viable cells but can penetrate cell membranes of dying or dead cells, in which it intercalates into double stranded nucleic acids. Dead cells which take up DAPI will fluoresce brightly around 461nm. DAPI may be excited by the UV or violet lasers although the UV laser is much more efficient. DAPI's emission spectra is very similar to the Pacific Blue (TM) dye.
  
  Supplied at a concentration of 1 mg/ml.

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 0.1-1.0ug/ml
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry Whole-Mount
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin

Reactivity Notes

Use in Human reported in scientific literature (PMID:33296752)

Packaging, Storage & Formulations

• Storage: Store at -20C in the dark. Avoid freeze-thaw cycles.
• Buffer: Water
• Preservative: No Preservative
• Concentration: Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Alternate Names for DAPI Solution

• 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride
• 4,6-Diamidino-2-phenylindole dihydrochloride
• DAPI dihydrochloride

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

Publications for DAPI Solution (NBP2-31156)(25)

Publications using NBP2-31156

• Buse J Einfluss einer ERK1/2-Resistenz auf Zellwachstum und Invasivit�t von triple-negativen Mammakarzinomzellen Thesis 2023-01-27
• Janssen L, Saranya M, Leinonen M et al. Vertically aligned carbon nanotube micropillars induce unidirectional chondrocyte orientation Carbon 2019-11-01 (IF/IHC, Human)
• Ely Cheikh Boussaty, Yuzuru Ninoyu, Leonardo R. Andrade, Qingzhong Li, Ryu Takeya, Hideki Sumimoto, Takahiro Ohyama, Karl J. Wahlin, Uri Manor, Rick A. Friedman, Karen P. Steel Altered Fhod3 expression involved in progressive high-frequency hearing loss via dysregulation of actin polymerization stoichiometry in the cuticular plate PLOS Genetics 2024-03-18 [PMID: 38498576]
• Chousal, JN;Morey, R;Srinivasan, S;Lee, K;Zhang, W;Yeo, AL;To, C;Cho, K;Garzo, VG;Parast, MM;Laurent, LC;Cook-Andersen, H; Molecular profiling of human blastocysts reveals primitive endoderm defects among embryos of decreased implantation potential Cell reports 2024-01-25 [PMID: 38277271]
• Saranya M, da Silva AM, Karjalainen H et al. Magnetic-Responsive Carbon Nanotubes Composite Scaffolds for Chondrogenic Tissue Engineering Advanced healthcare materials 2023-09-02 [PMID: 37660271] (IHC)
• Juras J, Webb M, Young L et al. In situ microwave fixation provides an instantaneous snapshot of the brain metabolome Cell Reports Methods 2023-04-01 [PMID: 37159672] (IHC-FrFl, Mouse)
• Juras JA PERIPHERAL AND CENTRAL GLUCOSE FLUX IN TYPE I DIABETES Thesis 2022-01-01 (IHC-Fr)
• Boussaty E, Ninoyu Y, Andrade L et al. Altered Fhod3 Expression Involved in Progressive High-Frequency Hearing Loss via Dysregulation of Actin Polymerization Stoichiometry in The Cuticular Plate bioRxiv 2023-07-25 [PMID: 37546952]
• Conroy LR, Clarke HA, Allison DB et al. Spatial metabolomics reveals glycogen as an actionable target for pulmonary fibrosis Nature communications 2023-05-13 [PMID: 37179348] (IHC-P)
• Berard AR, Brubaker DK, Birse K et al. Vaginal epithelial dysfunction is mediated by the microbiome, metabolome, and mTOR signaling Cell reports 2023-05-05 [PMID: 37149863] (ICC/IF)
• Sue YY Biological Correlates of Neuroimaging and a Systems Neuroimaging Approach to Examining Gene Environment Interactions in Psychiatric Disease Thesis 2021-01-01 (IF/IHC)
• Dervas E, Hepojoki J, Smura T, Prahauser B Python nidoviruses, more than respiratory pathogens bioRxiv 2020-01-01 (IHC-P)
• Ruckriegl S, Loris J, Wert K et al. Knockdown of G Protein-coupled Estrogen Receptor 1 (GPER1) Enhances Tumor-supportive Properties in Cervical Carcinoma Cells Cancer genomics & proteomics 2023-04-24 [PMID: 37093686] (ICC/IF, Human)
  
  Details:
  Dilutions: 1:1000
• Atzori MG, Ceci C, Ruffini F Et al. The Anti-Vascular Endothelial Growth Factor Receptor 1 (VEGFR-1) D16F7 Monoclonal Antibody Inhibits Melanoma Adhesion to Soluble VEGFR-1 and Tissue Invasion in Response to Placenta Growth Factor Cancers (Basel) 2022-11-26 [PMID: 36428669] (IHC-P)
  
  Details:
  Citation using the HRP version of this antibody.
• Jung Y, Cackowski FC, Yumoto K et al. Abscisic acid regulates dormancy of prostate cancer disseminated tumor cells in the bone marrow Neoplasia (New York, N.Y.) 2021-01-01 [PMID: 33296752] (FLOW, Human)
• Hellinger JW, SchOmel F, Buse JV et al. Identification of drivers of breast cancer invasion by secretome analysis: insight into CTGF signaling Sci Rep 2020-10-21 [PMID: 33087801] (ICC/IF, Human)
• Su X, Gu X, Zhang Z et al. Retinoic acid receptor gamma is targeted by microRNA-124 and inhibits neurite outgrowth Neuropharmacology 2019-06-03 [PMID: 31170403] (IF)
• Farombi EO, Awogbindin IO, Farombi TH et al. NEUROPROTECTIVE ROLE OF KOLAVIRON IN STRIATAL REDO-INFLAMMATION ASSOCIATED WITH ROTENONE MODEL OF PARKINSON'S DISEASE Neurotoxicology 2019-03-28 [PMID: 30930291] (ICC/IF)
• Yi SY, Barnett BR, Torres-Velazquez M et al. Detecting Microglial Density With Quantitative Multi-Compartment Diffusion MRI Front Neurosci 2019-02-19 [PMID: 30837826] (ICC/IF)
• Ferreira F, Raghunathan V, Luxardi G et al. Early redox activities modulate Xenopus tail regeneration. Nat Commun. 2018-10-16 [PMID: 30327466] (ICC/IF)
• Stenhouse C, Hogg CO, Ashworth CJ. Associations between fetal size, sex and placental angiogenesis in the pig Biol. Reprod. 2019-01-01 [PMID: 30137229] (ICC/IF, Porcine)
• Hansjosten I, Rapp J, Reiner L et al. Microscopy-based high-throughput assays enable multi-parametric analysis to assess adverse effects of nanomaterials in various cell lines Arch. Toxicol. 2017-11-08 [PMID: 29119250] (FLOW)
• Chousal J, Srinivasan S, Lee K et al. Molecular signatures associated with successful implantation of the human blastocyst bioRxiv 2023-05-10 (Immunocytochemistry/ Immunofluorescence)
  
  Details:
  Dilution: 1:500
• Chousal JN, Sohni A, Vitting-Seerup K et al. Progression of the pluripotent epiblast depends upon the NMD Factor UPF2 Development (Cambridge, England) 2022-10-18 [PMID: 36255229] (ICC/IF)
  
  Details:
  Dilution used in ICC 1:1000
• Gupta K, Levinsohn J, Linderman G et al. Single-Cell Analysis Reveals a Hair Follicle Dermal Niche Molecular Differentiation Trajectory that Begins Prior to Morphogenesis Dev. Cell 2018-12-19 [PMID: 30595533] (IHC-P)

Review for DAPI Solution (NBP2-31156) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Arun Satelli ICC Human 10/09/2014

Summary

• Application: Immunocytochemistry
• Sample Tested: Human Cancer cell line LM7
• Species: Human

FAQs for DAPI Solution (NBP2-31156). (Showing 1 - 1 of 1 FAQs).

1. What is the concentration of 1mL of  DAPI Solution?
   • The concentration of this solution is 1 mg/ml.