Dengue Virus 2 NS1 Antibody (969828) [Unconjugated]

Western blot shows recombinant Dengue Virus (DENV) 1, recombinant DENV2, recombinant DENV3, and recombinant DENV4. PVDF membrane was probed with 5 µg/mL of Mouse Anti-Viral Dengue Virus 2 NS1 Monoclonal Antibody (Catalog # MAB94391) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Dengue Virus 2 NS1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: V
• Applications: WB
• Clone: 969828
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: Please see the vial label for concentration. If unlisted please contact technical services.

Dengue Virus 2 NS1 Antibody (969828) [Unconjugated] Summary

• Immunogen: Human embryonic kidney cell line HEK293-derived recombinant Dengue Virus 2 NS1 protein
  Asp1-Ala352
  Accession # NP_739584
• Specificity: Detects recombinant Dengue Virus 2 NS1 protein in direct ELISAs and Western blots. In Direct Elisa and Western Blots, no cross-reactivity with recombinant Dengue Virus 1, 3 and 4 NS1 proteins was observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: Please see the vial label for concentration. If unlisted please contact technical services.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Dengue Virus 2 NS1 Antibody (969828) [Unconjugated]

• DEN-2
• Dengue NS1 protein
• Dengue NS1
• Dengue Virus 2 NS1
• Dengue virus Serotype 2
• Dengue virus Type 2
• Dengue Virus
• DENV2
• DENV2-NS1

Background

Dengue virus (DENV), the cause of Dengue fever, is a single positive-stranded RNA virus of the family Flaviviridae, genus Flavivirus. 5 closely related but antigenically distinct serotypes of the virus have been found: DENV-1, DENV-2, DENV-3, DENV-4 and DENV-5.  DENV has a genome of about 11,000 bases, encoding a single large polyprotein that is subsequently cleaved into several structural and non-structural peptides. The polyprotein is divided into three structural proteins, C, prM, E, seven non-structural proteins, NS1, NS2a, NS2b, NS3, NS4a, NS4b, NS5, and short non-coding regions on both the 5' and 3' ends. DENV nonstructural protein-1 (NS1) is a secreted glycoprotein that is absent from viral particles but accumulates in the supernatant and on the plasma membrane of cells during infection.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • NP_739584()