Digoxigenin Antibody (611532) [Unconjugated]

Western blot shows lysates of Goat IgG labeled with Digoxigenin. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Digoxigenin Monoclonal Antibody (Catalog # MAB75201) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for IgG labeled with Digoxigenin at approximately 25 and 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: All-Multi
• Applications: WB
• Clone: 611532
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Digoxigenin Antibody (611532) [Unconjugated] Summary

• Immunogen: KLH-coupled Digoxigenin
• Specificity: Detects Digoxigenin labeled proteins, nucleic acids and Digoxigenin conjugated primary and secondary antibodies.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Digoxigenin Antibody (611532) [Unconjugated]

• DIG
• Digoxigenin

Background

Digoxigenin is a hapten, a small molecule with high immunogenicity, that is used in many molecular biology applications similarly to other popular haptens such as DNP (dinitrophenol), biotin, and fluorescein. Digoxigenin is a steroid found exclusively in the flowers and leaves of the plant genus Digitalis. Digoxigenin can be introduced into proteins and nucleic acids for detection in a variety of assays, including ELISA, Immunohistochemistry, in situ hybridization, Southern blot, and Western blot.

1. Décarie, A.A. et al. (1994) Peptides 15(3):511.
2. Hauptmann, G. et al. (1994) Trends in Genetics 10(8):266.
3. Goodarzi, M.T. et al. (1995) Biochemical Society Transactions 23(2):168S.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.