Digoxigenin Antibody (611621) [Biotin]

Netrin-4 was detected in perfusion fixed frozen sections of mouse brain (nucleus accumbens) using Goat Anti-Mouse Netrin-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1132) conjugated to Digoxigenin at 15 µg/mL overnight at 4 °C. Tissue was stained using the Digoxigenin Biotinylated Monoclonal Antibody (brown; Catalog # BAM7520) at 3 µg/mL followed by Streptavidin-HRP incubation. Specific staining was localized to vasculature. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: All-Multi
• Applications: IHC
• Clone: 611621
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Biotin
• Concentration: LYOPH

Digoxigenin Antibody (611621) [Biotin] Summary

• Immunogen: KLH-coupled Digoxigenin
• Specificity: Detects Digoxigenin labeled proteins, nucleic acids and Digoxigenin conjugated primary and secondary antibodies.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Digoxigenin Antibody (611621) [Biotin]

• DIG
• Digoxigenin

Background

Digoxigenin is a hapten, a small molecule with high immunogenicity, that is used in many molecular biology applications similarly to other popular haptens such as DNP (dinitrophenol), biotin, and fluorescein. Digoxigenin is a steroid found exclusively in the flowers and leaves of the plant genus Digitalis. Digoxigenin can be introduced into proteins and nucleic acids for detection in a variety of assays, including ELISA, Immunohistochemistry, in situ hybridization, Southern blot, and Western blot.

1. Décarie, A.A. et al. (1994) Peptides 15(3):511.
2. Hauptmann, G. et al. (1994) Trends in Genetics 10(8):266.
3. Goodarzi, M.T. et al. (1995) Biochemical Society Transactions 23(2):168S.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.