Digoxigenin Antibody (611621) [Alexa Fluor® 488]

ATF6 was detected in immersion fixed A549 human lung carcinoma cell line using digoxigenin-labeled Rabbit Anti-Human/Mouse/Rat ATF6 Monoclonal Antibody (MAB71527) at 8 µg/mL for 3 hours at room temperature. Cells were stained using Anti-Mouse Anti-Digoxigenin Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC7520G) at 1:10 dilution and counterstained with DAPI (blue). Specific staining was localized to nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Netrin-4 was detected in perfusion fixed frozen sections of mouse brain (nucleus accumbens) using digoxigenin-conjugated Goat Anti-Mouse Netrin-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1132) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Digoxigenin Alexa Fluor® 488-conjugated Monoclonal Antibody (green; Catalog # IC7520G) at a final concentration of 1X (1:10 dilution) and counterstained with DAPI (blue). Specific staining was localized to vasculature. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

• Reactivity: All-Multi
• Applications: IHC, ICC/IF
• Clone: 611621
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

Digoxigenin Antibody (611621) [Alexa Fluor® 488] Summary

• Immunogen: KLH-coupled Digoxigenin
• Specificity: Detects Digoxigenin labeled proteins, nucleic acids and Digoxigenin conjugated primary and secondary antibodies.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 1:10 dilution
  • Immunohistochemistry 1:10 dilution

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Digoxigenin Antibody (611621) [Alexa Fluor® 488]

• DIG
• Digoxigenin

Background

Digoxigenin is a hapten, a small molecule with high immunogenicity, that is used in many molecular biology applications similarly to other popular haptens such as DNP (dinitrophenol), biotin, and fluorescein. Digoxigenin is a steroid found exclusively in the flowers and leaves of the plant genus Digitalis. Digoxigenin can be introduced into proteins and nucleic acids for detection in a variety of assays, including ELISA, Immunohistochemistry, in situ hybridization, Southern blot, and Western blot.

1. Décarie, A.A. et al. (1994) Peptides 15(3):511.
2. Hauptmann, G. et al. (1994) Trends in Genetics 10(8):266.
3. Goodarzi, M.T. et al. (1995) Biochemical Society Transactions 23(2):168S.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Digoxigenin Antibody (IC7520G)(2)

Publications using IC7520G

• Nan Wang, Zhidan Wang, Sofia Tzourtzou, Xu Wang, Xiuli Bi, Julia Leimeister et al. The plant nuclear lamina disassembles to regulate genome folding in stress conditions Nature Plants 2023-07-03 [PMID: 37400513]
• H Ishii, SK Vodnala, BR Achyut, JY So, MC Hollander, TF Greten, A Lal, L Yang miR-130a and miR-145 reprogram Gr-1+CD11b+ myeloid cells and inhibit tumor metastasis through improved host immunity Nat Commun, 2018-07-04;9(1):2611. 7/4/2018 [PMID: 29973593]