Digoxigenin Antibody (611621) [Unconjugated]

Netrin-4 was detected in perfusion fixed frozen sections of mouse brain (nucleus accumbens) using Goat Anti-Mouse Netrin-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1132) conjugated to Digoxigenin at 15 µg/mL overnight at 4 °C. Tissue was stained using 25 μg/mL Mouse Anti-Digoxigenin Monoclonal Antibody (Catalog # MAB7520) followed by incubation with NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007). Tissue was counter­stained with DAPI (blue). Specific staining was localized to vasculature. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

• Reactivity: All-Multi
• Applications: IHC
• Clone: 611621
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Digoxigenin Antibody (611621) [Unconjugated] Summary

• Immunogen: KLH-coupled Digoxigenin
• Specificity: Detects Digoxigenin labeled proteins, nucleic acids and Digoxigenin conjugated primary and secondary antibodies.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Digoxigenin Antibody (611621) [Unconjugated]

• DIG
• Digoxigenin

Background

Digoxigenin is a hapten, a small molecule with high immunogenicity, that is used in many molecular biology applications similarly to other popular haptens such as DNP (dinitrophenol), biotin, and fluorescein. Digoxigenin is a steroid found exclusively in the flowers and leaves of the plant genus Digitalis. Digoxigenin can be introduced into proteins and nucleic acids for detection in a variety of assays, including ELISA, Immunohistochemistry, in situ hybridization, Southern blot, and Western blot.

1. Décarie, A.A. et al. (1994) Peptides 15(3):511.
2. Hauptmann, G. et al. (1994) Trends in Genetics 10(8):266.
3. Goodarzi, M.T. et al. (1995) Biochemical Society Transactions 23(2):168S.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Digoxigenin Antibody (MAB7520)(4)

Publications using MAB7520

• Thompson, IAP;Saunders, J;Zheng, L;Hariri, AA;Maganzini, N;Cartwright, AP;Pan, J;Yee, S;Dory, C;Eisenstein, M;Vuckovic, J;Soh, HT; An antibody-based molecular switch for continuous small-molecule biosensing Science advances 2023-09-22 [PMID: 37738337] (Bioassay, N/A)
• MT Birnie, AK Short, GB de Carvalh, L Taniguchi, BG Gunn, AL Pham, CA Itoga, X Xu, LY Chen, SV Mahler, Y Chen, TZ Baram Stress-induced plasticity of a CRH/GABA projection disrupts reward behaviors in mice Nature Communications, 2023-02-25;14(1):1088. 2023-02-25 [PMID: 36841826] (Immunohistochemistry, Transgenic Mouse)
• Shao-bin Wang, Siddharth Narendran, Shuichiro Hirahara, Akhil Varshney, Felipe Pereira, Ivana Apicella et al. DDX17 is an essential mediator of sterile NLRC4 inflammasome activation by retrotransposon RNAs Science Immunology 12/24/2021 [PMID: 34860583]
• GL Cornelison, SA Levy, T Jenson, B Frost Tau-induced nuclear envelope invagination causes a toxic accumulation of mRNA in Drosophila Aging Cell, 2018-11-09;0(0):e12847. 2018-11-09 [PMID: 30411463] (IHC, Drosophila)