Recombinant E. coli Disulfide-bond isomerase Protein

SDS-Page: Recombinant E. coli Disulfide-bond isomerase Protein [NBC1-18366] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

• Reactivity: Ec
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant E. coli Disulfide-bond isomerase Protein Summary

• Description: An un-tagged recombinant protein corresponding to the amino acids 21-236 of E.coli Disulfide-bond isomerase
  
  

  Source: E.coli

  Amino Acid Sequence: MDDAAIQQTL AKMGIKSSDI QPAPVAGMKT VLTNSGVLYI TDDGKHIIQG PMYDVSGTAP VNVTNKMLLK QLNALEKEMI VYKAPQEKHV ITVFTDITCG YCHKLHEQMA DYNALGITVR YLAFPRQGLD SDAEKEMKAI WCAKDKNKAF DDVMAGKSVA PASCDVDIAD HYVLGVQLGV SGTPAVVLSN GTLVPGYQPP KEMKEFLDEH QKMTSGK

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 23.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Escherichia coli (strain B / BL21-DE3)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 7.5), 2 mM EDTA
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Background

Recombinant Disulfide Oxidoreductase (rDsbA), produced from E.Coli is a periplasmic protein and thioredoxin superfamily member which introduces disulfide bonds directly into substrate proteins by donating the disulfide bond in its active-site Cys30-Pro31-His32-Cys33 to a pair of cysteines in substrate proteins. DsbA consists of 208 amino acids containing signal peptide(1-19 amino acids). Recombinant DsbA (residues 20-208) was expressed in E. coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.