DNA Antibody (AC-30-10) - BSA Free

• Reactivity: Hu
• Applications: ICC/IF, IHC, WB
• Clone: AC-30-10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.1 mg/ml

DNA Antibody (AC-30-10) - BSA Free Summary

• Immunogen: Human DNA
• Specificity: This reacts with all forms of native and denatured DNA, in all DNA containing species tested so far. It also reacts with lambda DNA and synthetic DNA. It has also been found to react with the following cell lines, XLKE-A6, RVF-SMC, PTK-2, and HeLa.
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Purity: IgM purified

Applications/Dilutions

• Dilutions:
  • Immunoblotting
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10
  • Immunohistochemistry-Paraffin 1:10

Reactivity Notes

Predicted cross-reactivities: Insect, Chicken, Amphibia, Mouse, Rat

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 0.5% Bovine Serum Albumin
• Preservative: <0.1% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: IgM purified

Alternate Names for DNA Antibody (AC-30-10) - BSA Free

• Double stranded deoxyribonucleic acid
• Double stranded DNA
• dsDNA

Background

dsDNA (double stranded deoxyribonucleic acid) is the genetic material of all cells and many viruses and is a polymer of nucleotides. The monomer consists of phosphorylated 2-deoxyribose N-glycosidically linked to one of four bases, adenine, cytosine, guanine or thymine. These are linked together by 3',5'-phosphodiester bridges. In the Watson-Crick double-helix model, two complementary strands are wound in a right-handed helix and held together by hydrogen bonds between complementary base pairs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DNA Antibody (NB110-89473)(5)

Publications using NB110-89473

• Mittra I, Pal K, Pancholi N et Al. Cell-free chromatin particles released from dying host cells are global instigators of endotoxin sepsis in mice PLoS ONE 2020-03-04 [PMID: 32130239] (ICC/IF, Mouse)
• Bitto NJ, Chapman R, Pidot S et al. Bacterial membrane vesicles transport their DNA cargo into host cells Sci Rep 2017-08-01 [PMID: 28765539] (ICC/IF, Human)
• Pepin G, Nejad C, Thomas BJ et al. Activation of cGAS-dependent antiviral responses by DNA intercalating agents. Nucleic Acids Res 2017-01-09 [PMID: 27694309] (ICC/IF)
• Delgado L, Carrion O et al. The stack: a new bacterial structure analyzed in the Antarctic bacterium Pseudomonas deceptionensis M1(T) by transmission electron microscopy and tomography. PLoS One 2013-09-17 [PMID: 24039905] (IF/IHC, Bacteria)
  
  Details:
  Pseudomonas
• Perez-Cruz C, Carrion O, Delgado L et al. A new type of outer membrane vesicles produced by the Gram-negative bacterium Shewanella vesiculosa M7T: implications for DNA content Appl Environ Microbiol 2013-01-11 [PMID: 23315742]

FAQs for DNA Antibody (NB110-89473). (Showing 1 - 1 of 1 FAQs).

1. Is the DNA antibody AC-30-10 specific against double-stranded DNA or does it also bind to single-stranded DNA?
   • According to our datasheet, NB110-89473 reacts with all forms of native and denatured DNA, in all DNA containing species tested so far. It also reacts with lambda DNA and synthetic DNA. It has also been found to react with the following cell lines, XLKE-A6, RVF-SMC, PTK-2, and HeLa. It should bind both DNA forms.