DNA Polymerase epsilon catalytic subunit A Antibody (3C5.1) - BSA Free

Western Blot: DNA Polymerase epsilon catalytic subunit A Antibody (3C5.1) [NB100-115] - SDS-polyacrylamide gel analysis of HeLa DNA pol purified by Mono S FPLC. The Mono S fraction (0.1 mg) was electrophoresed on a 7% SDS-polyacrylamide gel and stained with silver.

Immunohistochemistry-Paraffin: DNA Polymerase epsilon catalytic subunit A Antibody (3C5.1) [NB100-115] - Analysis of a FFPE tissue section of human kidney using 1:200 dilution of DNA Polymerase epsilon catalytic subunit A [3C5.1] antibody. The staining was developed using HRP labeled anti-mouse secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.

Product Discontinued

DNA Polymerase epsilon catalytic subunit A Antibody (3C5.1) - BSA Free Summary

• Immunogen: Human DNA Polymerase epsilon purified from HeLa cells.
• Localization: Nuclear
• Specificity: This is specific to the human DNA pol epsilon and does not recognize DNA pol alpha, beta or delta.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: POLE
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry reported in scientific literature (PMID 29935943)
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10-1:500
  • Western Blot 5 ug/ml
• Application Notes: In WB, this DNA Polymerase epsilon (3C5.1) antibody detects a single band of 220 kDa (in HeLa extracts) representing the catalytic subunit of DNA Polymerase epsilon. NB100-115 will immunoprecipitate, but only in the absence of salt and even then poorly. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 220 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mink samples. This antibody does not work on Drosophilia or the baculovirus host cell, and weakly, if at all, on S. cerivisae POLE.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DNA Polymerase epsilon catalytic subunit A Antibody (3C5.1) - BSA Free

• DKFZp434F222
• DNA polymerase epsilon catalytic subunit A
• DNA polymerase II subunit A
• EC 2.7.7
• EC 2.7.7.7
• POLE1FLJ21434
• polymerase (DNA directed), epsilon

Background

DNA Polymerase epsilon, also called DNA polymerase epsilon subunit 3 (POLE3) is a histone-fold protein which interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. DNA Polymerase epsilon generates a complex with DNA polymerase epsilon subunit CHRAC1 and associates with naked DNA, which is then incorporated into chromatin, facilitated through the nucleosome-remodeling activity of ISWI/SNF2H and ACF1. DNA Polymerase epsilon is a component of the epsilon DNA polymerase complex consisting of four subunits: POLE, POLE2, POLE3 and POLE4, wherein, the interaction with POLE4 is a prerequisite for further binding with POLE or POLE2. DNA Polymerase epsilon interacts with CHRAC1, and together with CHRAC1, ACF1 and ISWI/SNF2H proteins, it forms the ISWI chromatin-remodeling complex, CHRA. DNA Polymerase epsilon histone-fold protein dimers combine within larger enzymatic complexes for facilitating the processes of DNA packaging, replication, and transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DNA Polymerase epsilon catalytic subunit A Antibody (NB100-115)(9)

Publications using NB100-115

• Gaspar J M, Mendes N F et al. Downregulation of HIF complex in the hypothalamus exacerbates diet-induced obesity. Brain Behav Immun 2018-01-10 [PMID: 29935943] (IF/IHC, Mouse)
• Asahara H, Li Y, Fuss J, Haines DS, Vlatkovic N, Boyd MT, Linn S. Stimulation of human DNA polymerase epsilon by MDM2. Nucleic Acids Res;31(9):2451-9. 2003-05-01 [PMID: 12711691]
• Liu W, Linn S. Proteolysis of the human DNA polymerase epsilon catalytic subunit by caspase-3 and calpain specifically during apoptosis. Nucleic Acids Res;28(21):4180-8. 2000-11-01 [PMID: 11058115]
• Vlatkovic N, Guerrera S, Li Y, Linn S, Haines DS, Boyd MT. MDM2 interacts with the C-terminus of the catalytic subunit of DNA polymerase epsilon. Nucleic Acids Res;28(18):3581-6. 2000-09-15 [PMID: 10982879]
• Li Y, Pursell ZF, Linn S. Identification and cloning of two histone fold motif-containing subunits of HeLa DNA polymerase epsilon. J Biol Chem;275(30):23247-52. 2000-07-28 [PMID: 10801849]
• Kramata P, Downey KM, Paborsky LR. Incorporation and excision of 9-(2-phosphonylmethoxyethyl)guanine (PMEG) by DNA polymerase delta and epsilon in vitro. J Biol Chem;273(34):21966-71. 1998-08-21 [PMID: 9705337]
• Islas L, Fairley CF, Morgan WF. DNA synthesis on discontinuous templates by human DNA polymerases: implications for non-homologous DNA recombination. Nucleic Acids Res;26(16):3729-38. 1998-08-15 [PMID: 9685489]
• Li Y, Asahara H, Patel VS, Zhou S, Linn S. Purification, cDNA cloning, and gene mapping of the small subunit of human DNA polymerase epsilon. J Biol Chem;272(51):32337-44. 1997-12-19 [PMID: 9405441]
• Chui, G, Linn, S. Further characterization of HeLa DNA polymerase epsilon. J Biol Chem;270(14):7799-808. 1995-04-07 [PMID: 7536191] (WB, Mouse)

Bioinformatics

• Gene Symbol: POLE