DNA/RNA Damage Antibody (15A3) [Alexa Fluor® 700]

• Reactivity: Hu, Mu, All-NA
• Applications: DB, Flow
• Clone: 15A3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 700

DNA/RNA Damage Antibody (15A3) [Alexa Fluor® 700] Summary

• Immunogen: 8-hydroxy-guanosine-BSA and-casein conjugates
• Specificity: Recognizes markers of oxidative damage to DNA (8-hydroxy-2'-deoxyguanosine, 8-hydroxyguanine and 8-hydroxyguanosine).
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • Flow Cytometry
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Use in Human reported in scientific literature (PMID:33482333) Mouse reactivity reported in scientific literature (PMID: 31226694).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for DNA/RNA Damage Antibody (15A3) [Alexa Fluor® 700]

• DNA/RNA Oxidative Damage Markers Monoclonal Antibody

Background

DNA or RNA damage is due to environmental factors and normal metabolic processes inside the cell, that then hinder the ability of the cell to carry out its functions. There are four main types of DNA due to endogenous cellular processes and they are oxidation, alkylation, hydrolysis and mismatch of the bases. During the oxidation of bases, highly reactive chemical entities collectively known as RONS, occurs. RONS stands for reactive oxygen and nitrogen species and includes nitric oxide, superoxide, hydroxyl radical, hydrogen peroxide and peroxynitrite. Numerous studies have shown that RONS causes a variety of issues including DNA damage (1). 8-hydroxyguanine, 8-hydroxy-2'-deoxyguanonsine and 8-hydroxyguanosine are all RNA and DNA markers of oxidative damage. 8-hydroxy-2'-guanosine is produced by reactive oxygen and nitrogen species including hydroxyl radical and peroxynitrite. Specifically its high biological relevance is due to its ability to induce G to T transversions, which is one of the most frequent somatic mutations (2). 8-hydroxy-guanine has been the most frequently studied type of DNA base damage, with studies in diabetes, and cancer. Base modifications of this type arise from radical-induced hydroxylation and cleavage reactions of the purine ring (3, 4). And finally, 8-hydroxy-guanosine, like 8-hydroxy-2'-guanosine, induces a mutagenic transversion of G to T in DNA. Its role has specifically been tested in the development of diabetes, hypertension and strokes (5, 6, and 7).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.