Doublecortin Antibody (3E1) - BSA Free

Western Blot: Doublecortin Antibody (3E1) [NBP1-92684] - Analysis of rat whole brain lysates using mouse mAb to Doublecortin (DCX), NBP1-92684, dilution 1:1,000 in green: [1] protein standard (red), [2] adult rat brain, [3] embryonic E20 rat brain. Strong bands at 40, 45kDa correspond to DCX protein, detected only in the developing brain.

Western Blot: Doublecortin Antibody (3E1) [NBP1-92684] - Analysis of rat whole brain lysates using mouse mAb to Doublecortin (DCX), NBP1-92684, dilution 1:1,000 in green: [1] protein standard (red), [2] adult rat brain, [3] embryonic E20 rat brain. Strong bands at 40, 45kDa correspond to DCX protein, detected only in the developing brain.

Immunocytochemistry/Immunofluorescence: Doublecortin Antibody (3E1) [NBP1-92684] - Analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to Doublecortin (DCX), NBP1-92684, dilution 1:1,000 in red, and costained with chicken pAb to Microtubule Associated Protein2 (MAP2), dilution 1:10,000 in green. The blue is DAPI staining of nuclear DNA. DCX antibody reveals strong cytoplasmic staining in a population of small developing neurons and their processes, while MAP2 antibody stains dendrites and perikarya of mature neurons. Therefore DCX antibody is an excellent marker of developing neuronal cells.

Immunocytochemistry/Immunofluorescence: Doublecortin Antibody (3E1) [NBP1-92684] - Rat brain neural cultures stained with NBP1-92684 (green), a chicken polyclonal antibody to GFAP (NB300-213, red) and DNA (blue). The NBP1-92684 antibody reveals strong cytoplasmic staining in a population of small developing neurons. These cells are often found in small clumps as in these cultures, as in this example. Note that they are not positive for MAP2, which is characteristic of more mature neurons, and that the mature neuron shown is negative for NBP1-92684. This Doublecortin antibody is therefore an excellent marker of developing neuronal cells.

• Reactivity: Hu, Mu, Rt, Po, Bv, Eq
• Applications: WB, ICC/IF, IHC
• Clone: 3E1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Doublecortin Antibody (3E1) - BSA Free Summary

• Immunogen: Recombinant full length human Lis-A isoform of Doublecortin purified from E. coli. [UniProt# O43602]
• Localization: Cytoplasm. Cell projection. Note: Localizes at neurite tips.
• Marker: Immature Neuronal Marker
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: DCX
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:1000
  • Immunohistochemistry 1:1000
  • Western Blot 1:10000
• Application Notes: This Doublecortin (3E1) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry, and Western blot, where bands can be seen at approximately 35 and 45 kDa.
• Theoretical MW: 35-45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50% PBS, 50% glycerol
• Preservative: 0.035% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Doublecortin Antibody (3E1) - BSA Free

• DBCN
• Doublecortex
• Doublecortin
• Doublin
• FLJ51296
• Lissencephalin-X
• LISX
• Lis-X
• X-linked (doublecortin)

Background

Doublecortin was originally discovered since defects in the gene encoding it are causative of X-linked lissencephaly, a rare group of brain malformations resulting in a smooth cerebral cortex caused by aberrant neuronal migration during development. The name Doublecortin comes from the unusual layering of the cortex in this form of lissencephaly, which appears to have a second deep cortical layer of neurons. This layer consists of neurons which did not migrate from the subventricular zone to the normal cortical layer. Patients with this defect suffer from seizures and mental retardation. The HGNC name for Doublecortin is DCX, and it is also known as Doublin, Lissencephalin-X, DBCN and Lis-X. Four proteins encoded by the DCX produce bands of about 35kDa and 45kDa on Western blots. The 45kDa form is known as Lis-XA while the smaller forms are generated by alternate transcription, are all missing the first 81 amino acids of Lis-XA, and are referred to as Lis-XB, Lis-XC, Lis-XD. There are minor amino acid sequence differences between these three smaller isoforms. All of these protein contain two so-called Doublecortin domains, each about 90 amino acids long, which are believed to function in binding to microtubules, a C-terminal serine and proline rich region which may become phosphorylated in vivo. The doublecortin protein appears to function as a microtubule and actin binding protein and may interact with Lis-1, a member of the beta-transducin or WD protein family, a protein mutations of which are also associated with lissencephaly. DCX is expressed very early in neuronal development, as neuroblasts become post-mitotic, but is lost as neurons mature. Developing neurons start to lose DCX expression about the time that they begin to express NeuN, a neuronal specific protein characteristic of more mature neurons, now known to correspond to the RNA binding protein Fox3. Antibodies to DCX are used to identify stem cells in sections and in tissue culture, and to see if neurogenesis is taking place.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Doublecortin Antibody (NBP1-92684)(2)

Publications using NBP1-92684

• Obara K, Shirai K, Hamada Y et al. Direct implantation of hair-follicle-associated pluripotent (HAP) stem cells repairs intracerebral hemorrhage and reduces neuroinflammation in mouse model PloS one 2023-01-13 [PMID: 36638123] (ICC/IF, Mouse)
  
  Details:
  Dilution used in IHC-P 1:1000
• Kubis-Kubiak A, Wiatrak B, Piwowar A. Et al. Hyper-glycemia and insulinemia induce morphological changes and modulate secretion of S100B, S100A8, amyloid b 1-40 and amyloid b 1-42, in a model of human dopaminergic neurons Biomed Pharmacother 2022-10-18 [PMID: 36257211] (ICC/IF, Rat)
  
  Details:
  Citation using the DyLight 488 version of this antibody.

Bioinformatics

• Gene Symbol: DCX
• Entrez:
  • 1641(Human)
• Uniprot:
  • O43602()