DPPIV/CD26 Antibody (OTI11D7)

Western Blot: DPPIV/CD26 Antibody (11D7) [NBP2-02154] - Western Blot of DPP4 protein level by using anti-DPP4 antibody in mouse MLE15 cells.

Immunocytochemistry/Immunofluorescence: DPPIV/CD26 Antibody (OTI11D7) [NBP2-02154] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY CD26.

Immunohistochemistry: DPPIV/CD26 Antibody (11D7) [NBP2-02154] - Lungs harvested from BALB/c mice at day 3. Dilution: 1:800

Flow Cytometry: DPPIV/CD26 Antibody (11D7) [NBP2-02154] - Analysis of Hela cells, using anti-CD26 antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Western Blot: DPPIV/CD26 Antibody (11D7) [NBP2-02154] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD26 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD26.

Immunohistochemistry-Paraffin: DPPIV/CD26 Antibody (11D7) [NBP2-02154] - Staining of paraffin-embedded liver tissue using anti-CD26 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI11D7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

DPPIV/CD26 Antibody (OTI11D7) Summary

• Immunogen: Full length human recombinant protein of human DPP4 (NP_001926) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: DPP4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:500
• Theoretical MW: 88.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DPPIV/CD26 Antibody (OTI11D7)

• ADABP
• ADCP-2
• ADCP2DPP IV
• Adenosine deaminase complexing protein 2TP103
• CD26 antigen
• CD26
• CD26T-cell activation antigen CD26
• dipeptidyl peptidase 4
• Dipeptidyl peptidase IV
• dipeptidylpeptidase 4
• dipeptidyl-peptidase 4
• dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2)
• DPP4
• DPPIV
• EC 3.4.14.5

Background

The protein encoded by this gene is identical to adenosine deaminase complexing protein-2, and to the T-cell activation antigen CD26. It is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DPP4
• Entrez:
  • 1803(Human)