DSPP Recombinant Protein Antigen

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Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-85242PEP
    • Availability
      Product Discontinued

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DSPP Recombinant Protein Antigen Summary

Description
A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human DSPP.

Source: E. coli

Amino Acid Sequence: DSSIGQNSDSKEYYDPEGKEDPHNEVDGDKTSKSEENSAGIPEDNGSQRIEDTQKLNHRESKRVENRITKESETHAVGKSQDKGIEIKGPSSGNRN

Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

Source
E. coli
Protein/Peptide Type
Recombinant Protein Antigen
Gene
DSPP
Purity
>80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

Dilutions
  • Antibody Competition 10 - 100 molar excess
Application Notes
This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP1-85242.

It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 1M Urea, pH 7.4.
Preservative
No Preservative
Purity
>80% by SDS-PAGE and Coomassie blue staining

Alternate Names for DSPP Recombinant Protein Antigen

  • dentin phosphoprotein
  • dentin sialophosphoprotein
  • dentin sialoprotein
  • DFNA39
  • DMP3
  • DTDP2

Background

The DSPP gene encodes two principal proteins of the dentin extracellular matrix of the tooth. The preproprotein is secretedby odontoblasts and cleaved into dentin sialoprotein and dentin phosphoprotein. Dentin phosphoprotein is thought to beinvolved in the bi

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Additional DSPP Products

Array NBP1-85242PEP

Research Areas for DSPP Protein (NBP1-85242PEP)

Find related products by research area.

Blogs on DSPP.

BSP and Osteopontin Antibodies: A Case of SIBLING Rivalry
Bone and dentin are closely related tissues, formed when a type I collagen-rich extracellular matrix (ECM) is secreted from the osteoblasts or odontoblasts and subsequently mineralized. This process is tightly regulated by type I collagen plus a numbe...  Read full blog post.

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Bioinformatics

Gene Symbol DSPP