dsRNA Antibody (J2) - Azide and BSA Free

Immunohistochemistry-NBP3-11395-dsRNA Antibody (J2) - Azide and BSA Free-Antibody J2 (1:250 dilution, 4 microgram/ml) reveals the colocalisation of dsRNA (labelled in green) and Hepatitis C virus core protein (labelled in red), indicating the location of putative virus assembly sites (arrows).

• Reactivity: All-Multi
• Applications: WB, DB, ELISA, Flow, ICC/IF, IHC, AP
• Clone: J2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

dsRNA Antibody (J2) - Azide and BSA Free Summary

• Immunogen: Female DBA/2 mice were injected intraperitonially with a mixture of 50 ug L-dsRNA and 75 ug methylated bovine serum albumin, emulsified in complete Freund's adjuvant. After several boosts spleen cells were fused with Sp2/0-Agl4 myeloma cells to generate the hybridoma clone.
• Specificity: This antibody recognises double-stranded RNA (dsRNA) provided that the length of the helix is greater than or equal to 40 bp. dsRNA-recognition is independent of the sequence and nucleotide composition of the antigen. All naturally occurring dsRNAs investigated up to now (40-50 species) as well as poly(I).poly(C) and poly(A).poly(U) have been recognised by this antibody, although in some assays its affinity to poly(I).poly(C) is about 10 times lower than that to other dsRNA antigens.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • ELISA
  • Flow Cytometry
  • Immunoaffinity Purification
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Western Blot
• Application Notes: This antibody can be used for ELISA, dsRNA-immunoblotting, immunoaffinity chromatography and in certain systems also for immunohistochemistry. The optimum working dilution of the antibody for any specific application should be established by titration.
  
  Please note that nucleic acid separation prior to dsRNA-immunoblotting must be carried out by polyacrylamide gel electrophoresis, because the sensitivity of detection is considerably lower after blotting from agarose gels.
  
  Not for use for clinical purposes. For in vitro use only.
  
  Immuno-affinity-chromatography.

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at -20C prior to opening. Aliquot reconstituted liquid and and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing.
• Buffer: Lyophilised from PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: The lyophilised sample should be reconstituted with 200 ul (500 ul for 500 ug size) sterile distilled water. The mAb will then be in PBS without any stabilisers at a concentration of 1 mg/ml.

Notes

After reconstitution antibodies should be aliquoted and stored at -20C or -70C. After adding 10 mM sodium azide undiluted antibody can also be stored at 4C for a short period of time. For long term storage the mAb should be kept frozen. Repeated freezing/thawing cycles should be avoided. When kept lyophilized the product will remain stable for at least 5 years at -20C or -70C. This ds-RNA antibody was manufactured by Scicon.

Alternate Names for dsRNA Antibody (J2) - Azide and BSA Free

• Double-stranded RNA

Background

Double-stranded RNA (dsRNA)antibodies have been used extensively to detect and characterise plant and animal viruses with dsRNA genomes or intermediates. J2 can be used to detect dsRNA intermediates of viruses as diverse as Hepatitis C virus, Dengue virus, rhinovirus, Chikungunya virus, Rabies virus, Polio virus, Classic swine fever virus, Brome mosaic virus and many more in cultured cells and also in fixed paraffin-embedded histological samples.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for dsRNA Antibody (NBP3-11395)(1)

Publication using NBP3-11395

• Cali M. McEntee, Alyssa N. Cavalier, Thomas J. LaRocca ADAR1 suppression causes interferon signaling and transposable element transcript accumulation in human astrocytes Frontiers in Molecular Neuroscience 2023-10-25 [PMID: 38035265]