Recombinant Human EGF Protein

SDS-Page: Human EGF Protein [NBP2-34952]

SDS-Page: Human EGF Protein [NBP2-34952]

• Reactivity: Hu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Human EGF Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 53 amino acids corresponding to EGF Source: E. coli

  Uniprot ID: P01133

  Amino Acid Sequence: NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR

• Details of Functionality: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1 ng/ml, corresponding to a specific activity of > 1.0 x 10^6 IU/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: EGF
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of EGF as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 6.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Human EGF Protein

• beta-urogastrone
• EGF
• epidermal growth factor (beta-urogastrone)
• epidermal growth factor
• hEGF
• HOMG4
• pro-epidermal growth factor
• URG
• Urogastrone

Background

EGF R (ErbB1) is a receptor tyrosine kinase that is widely expressed on epithelial cells and is required for epithelial cell development and proliferation. EGF R binds the EGF family ligands: EGF, Amphiregulin, TGF-alpha, Betacellulin, Epiregulin, HB-EGF, and Epigen. Ligand binding induces EGF R homodimerization as well as heterodimerization with ErbB2, ErbB3, or ErbB4. EGF R signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis. Alterations in the structure, expression, and signaling of EGF R are involved in the development and metastasis of a wide variety of cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for EGF Recombinant Protein (NBP2-34952)(16)

Publications using NBP2-34952

• Lovric A, Rassolie A, Alam S et al. Single-cell sequencing deconvolutes cellular responses to exercise in human skeletal muscle Communications biology 2022-10-22 [PMID: 36273106]
• Pan J, Sheng S, Ye L et al. Extracellular vesicles derived from glioblastoma promote proliferation and migration of neural progenitor cells via PI3K-Akt pathway Cell communication and signaling : CCS 2022-01-12 [PMID: 35022057]
• Xu C, Sun W, Liu J Et al. MiR-342-3p inhibits LCSC oncogenicity and cell stemness through HDAC7/PTEN axis Inflammation research : official journal of the European Histamine Research Society ... [et al.] 2021-11-29 [PMID: 34842937]
• Freed-Pastor WA, Lambert LJ, Ely ZA Et al. The CD155/TIGIT axis promotes and maintains immune evasion in neoantigen-expressing pancreatic cancer Cancer cell 2021-07-27 [PMID: 34358448]
• Robilliard LD, Yu J, Anchan A et al. Comprehensive analysis of inhibitory checkpoint ligand expression by Glioblastoma cells Immunol Cell Biol 2020-11-20 [PMID: 33217047]
• Wucheng Wang, Huanjun Chen, Lei Zhang et al. A fucoidan from Nemacystus decipiens disrupts angiogenesis through targeting bone morphogenetic protein 4. Carbohydr Polym 2016-06-25 [PMID: 27083822]
• Liu, Wanjun PhD, Wang et al. CYP2J2 Overexpression Increases EETs and Protects Against HFD-Induced Atherosclerosis in ApoE−/− Mice. Journal of Cardiovascular Pharmacology 2016-06-01 [PMID: 26859195]
• Yan Luan, Dafei Chai, Jianjian Peng et al. A fully human monoclonal antibody targeting PD-L1 with potent anti-tumor activity. Int Immunopharmacol 2016-02-01 [PMID: 26773772]
• Jiayin Hou, Liang Liu, Qian Zhu et al. MicroRNA‐185 inhibits angiogenesis in human microvascular endothelial cells through targeting stromal interaction molecule 1. Cell Biol Int 2016-03-01 [PMID: 26694763]
• Yu Ma, Fu-Chun Guo, Wei Wang et al. K-ras gene mutation as a predictor of cancer cell responsiveness to metformin. Mol Med Rep 2013-09-01 [PMID: 23877793]
• Xia Chen, Fei Xiao, Ying Wang et al. Structure-activity relationship study of WSS25 derivatives with anti-angiogenesis effects. Glycoconj J 2012-08-01 [PMID: 22847113]
• Yuxia Xu, Qun Dong, Hong Qiu et al. A homogalacturonan from the radix of Platycodon grandiflorum and the anti-angiogenesis activity of poly-/oligogalacturonic acids derived therefrom. Carbohydr Res 2011-09-27 [PMID: 21700274]
• Xiaokun Shen, Jianping Fang, Xiaofen Lv et al. Heparin Impairs Angiogenesis through Inhibition of MicroRNA-10b. J Biol Chem 2011-07-29 [PMID: 21642433]
• Yuxia Xu, Qun Dong, Hong Qiu et al. Structural Characterization of an Arabinogalactan from Platycodon grandiflorum Roots and Antiangiogenic Activity of Its Sulfated Derivative. Biomacromolecules 2010-10-11 [PMID: 20839877]
• Han L, Yan L, Wang K et al. Tough, self-healable and tissue-adhesive hydrogel with tunable multifunctionality. NPG Asia Materials 2017-04-14
• Hong Qiu, Bo Yang, Zhi-Chao Pei, et al. WSS25 Inhibits Growth of Xenografted Hepatocellular Cancer Cells in Nude Mice by Disrupting Angiogenesis via Blocking Bone Morphogenetic Protein (BMP)/Smad/Id1 Signaling. J Biol Chem 2010-10-15 [PMID: 20679340]

Review for EGF Recombinant Protein (NBP2-34952) (1)

Average Rating: 5

(Based on 1 review)

reviewed by: Verified Customer SDS-PAGE Control 01/04/2017

Summary

• Application: SDS-PAGE Control
• Comments: I ranked this protein with 5 stars because the cell differentiation was successfully performed.

Comments

• Comments: SDS PAGE gel with Coomassie blue staining showing detection of Human EGF protein (NBP2-34952-100 ug ) (5 ug).

Bioinformatics

• Gene Symbol: EGF
• Uniprot:
  • P01133()
  • P01133()
  • P01133()