Recombinant Human EGF Protein

SDS-Page: Recombinant Human EGF Protein [NBP2-61377]

SDS-Page of Recombinant Human EGF Protein

• Reactivity: Hu
• Applications: WB, Bioactivity
• Concentration: LYOPH

Recombinant Human EGF Protein Summary

• Description: A single glycosylated polypeptide chain containing 51 amino acids corresponding to EGF Source: Yeast

  Uniprot ID: P01133

  Amino Acid Sequence: NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW E

• Details of Functionality: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 x 10^7 IU/mg.
• Source: Yeast
• Protein/Peptide Type: Recombinant Protein
• Gene: EGF
• Purity: >98%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 0.1 EU/ug of EGF as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Western Blot
• Theoretical MW: 6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >98%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Human EGF Protein

• beta-urogastrone
• EGF
• epidermal growth factor (beta-urogastrone)
• epidermal growth factor
• hEGF
• HOMG4
• pro-epidermal growth factor
• URG
• Urogastrone

Background

Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: EGF