Recombinant Mouse EGF Isoform 1 His Protein

Bioactivity: Recombinant Mouse EGF Isoform 1 Protein [NBP2-61981] - Mouse EGF in a cell proliferation assay using mouse Balb/3T3 cell. The ED50 range = 1 ng/ml.

SDS-Page: Recombinant Mouse EGF Isoform 1 Protein [NBP2-61981] - 15% SDS-PAGE (3ug)

Product Discontinued

Recombinant Mouse EGF Isoform 1 His Protein Summary

• Description: A Biologically active recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 977-1029 of Mouse EGF
  
  

  Source:E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMNSYPGC PSSYDGYCLN GGVCMHIESL DSYTCNCVIG YSGDRCQTRD LRWWELR

• Details of Functionality: Mouse EGF in a cell proliferation assay using mouse Balb/3T3 cell. The ED50 range is less than or equal to 1 ng/ml.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: EGF
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: < 1 EU per 1ug of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 8.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 0.1 M NaCl, 2 mM DTT, 10% glycerol
• Preservative: No Preservative
• Concentration: 0.25 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Mouse EGF Isoform 1 His Protein

• beta-urogastrone
• EGF
• epidermal growth factor (beta-urogastrone)
• epidermal growth factor
• hEGF
• HOMG4
• pro-epidermal growth factor
• URG
• Urogastrone

Background

Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: EGF