![Immunofluorescence: eGFP Antibody [NBP2-59183] - A mitotic human HeLa cell, stably expressing a GFP-CENP-A fusion protein, was fixed with 4% formaldehyde and processed for immunofluorescence using the antibody against EGFP , diluted 1:800, and a monoclonal antibody against HEC1, followed by a CY2-labeled anti-goat and a CY3-labeled anti-mouse antibody, respectively. DNA was visualized using Hoechst staining. Images of the cell were taken with a Deltavision microscope. The images shown in Figure represent a projection of approximately 30 0.2 um optical sections.](http://images.novusbio.com/fullsize/eGFP-Antibody-Immunofluorescence-NBP2-59183-img0001.jpg "Immunofluorescence: eGFP Antibody [NBP2-59183] - A mitotic human HeLa cell, stably expressing a GFP-CENP-A fusion protein, was fixed with 4% formaldehyde and processed for immunofluorescence using the antibody against EGFP , diluted 1:800, and a monoclonal antibody against HEC1, followed by a CY2-labeled anti-goat and a CY3-labeled anti-mouse antibody, respectively. DNA was visualized using Hoechst staining. Images of the cell were taken with a Deltavision microscope. The images shown in Figure represent a projection of approximately 30 0.2 um optical sections.")
| Reactivity | All-NASpecies Glossary |
| Applications | ICC/IF |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Immunogen | The exact immunogen is propietary information. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide and 0.05% ProClin 300 |
| Purity | Affinity purified |
| Publication using NBP2-59183 | Applications | Species |
|---|---|---|
| Hou L, Ye Y, Gou H et al. A20 inhibits periodontal bone resorption and NLRP3-mediated M1 macrophage polarization Experimental cell research 2022-06-15 [PMID: 35714941] (IF/IHC) | IF/IHC |
Secondary Antibodies |
Isotype Controls |
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The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura. It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol... Read full blog post. |
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