Recombinant eGFP Protein

SDS-Page: Human GFP Protein [NBP2-34923] - GFP Protein [NBP2-34923]

• Applications: WB, PAGE
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant eGFP Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 239 amino acids corresponding to eGFP Source: E. coli

  Uniprot ID: P42212

  Amino Acid Sequence: MVSKGEELFT GVVPILVELD GDVNGHKFSV SGEGEGDATY GKLTLKFICT TGKLPVPWPT LVTTLTYGVQ CFSRYPDHMK QHDFFKSAMP EGYVQERTIF FKDDGNYKTR AEVKFEGDTL VNRIELKGID FKEDGNILGH KLEYNYNSHN VYIMADKQKN GIKVNFKIRH NIEDGSVQLA DHYQQNTPIG DGPVLLPDNH YLSTQSALSK DPNEKRDHMV LLEFVTAAGI TLGMDELYK

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of eGFP as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • SDS-Page
  • Western Blot
• Application Notes: Use in WB reported in scientific literature (PMID:10.1007/s00299-021-02700-w)
• Theoretical MW: 26.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant eGFP Protein

• Enhanced Green Fluorescent Protein
• GFP
• Green Fluorescent Protein

Background

Green fluorescent protein (GFP) here refers to the protein first purified from jellyfish Aequorea victoria, though many other organisms have similar proteins. It is a 26.9 kDa protein (composed of 238 a.a. residues) that shows green fluorescence in shortwave light (blue to ultraviolet). Despite of wild-type GFP, many mutants of GFP have been engineered for wider usage in research. Enhanced GFP (eGFP) has S65T and F64L mutations, which make GFP show increased fluorescence and fold more efficiently under 37 degrees C, respectively. eGFP allows the use of GFP in mammalian cells. In A. Victoria, GFP plays roles as an energy transfer acceptor. It has long been used in cell and molecular biology as a reporter of gene expression. GFP can also been applied as a molecular thermometer to measure temperature accurately in fluids.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for eGFP Recombinant Protein (NBP2-34923)(4)

Publications using NBP2-34923

• Nakazato Y, Otaki JM Protein Delivery to Insect Epithelial Cells In Vivo: Potential Application to Functional Molecular Analysis of Proteins in Butterfly Wing Development Biotech (Basel (Switzerland)) 2023-04-16 [PMID: 37092472]
• Simoes V, Cizubu BK, Harley L et al. Redox-sensitive E2 Rad6 controls cellular response to oxidative stress via K63-linked ubiquitination of ribosomes Cell reports 2022-05-24 [PMID: 35613580] (WB)
• Acanda Y, Welker S, Orbovic V, Levy A A simple and efficient agroinfiltration method for transient gene expression in Citrus Plant cell reports 2021-05-04 [PMID: 33948685] (WB)
• Yung-Hen Hsu, Chang-Tun Lin, Yi-Hsun Yu. Dual delivery of active antibactericidal agents and bone morphogenetic protein at sustainable high concentrations using biodegradable sheath-core-structured drug-eluting nanofibers. Int J Nanomedicine 2016-08-17 [PMID: 27574423]

Bioinformatics

• Uniprot:
  • P42212()