eIF5A Antibody (4D1) - BSA Free

Western Blot: eIF5A Antibody (4D1) [NBP2-59403] - Lane 1: Jurkat cell lysates, Lane 2: 293T cell lysates, Lane 3: HeLa cell lysates, Lane 4: MCF7 cell lysates, Lane 5: NIH-3T3 cell lysates

Immunocytochemistry/Immunofluorescence: eIF5A Antibody (4D1) [NBP2-59403] - Analysis of EIF5A in Balb/3T3 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human EIF5A antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green)

Flow Cytometry: eIF5A Antibody (4D1) [NBP2-59403] - Analysis of EIF5A in Balb/3T3 cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line)

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF
• Clone: 4D1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

eIF5A Antibody (4D1) - BSA Free Summary

• Immunogen: Recombinant human eIF5A (1-154aa) purified from E. coli
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: EIF5A
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1:10-1:1015
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:500

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for eIF5A Antibody (4D1) - BSA Free

• eIF4D
• eIF-4D
• eIF5A
• EIF-5A
• EIF5A1
• eIF-5A1
• eIF-5A-1
• eIF5AI
• Eukaryotic initiation factor 5A isoform 1
• eukaryotic initiation factor 5A
• eukaryotic translation initiation factor 5A
• eukaryotic translation initiation factor 5A-1
• MGC104255
• MGC99547
• Rev-binding Factor

Background

The human eukaryotic translation initiation factor 5A (eIF5A) is an 18 kDa protein. eIF5A precursor [(ec-eIF-5A(lys)] is the only cellular protein known to contain a specific lysine residue which is transformed into the unique amino acid hypusine [N-(4-amino-2-hydroxybutyl)-lysine] by a series of post translational reactions. eIF5A promotes the formation of the first peptide bond during the initial stage of protein synthesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EIF5A