Recombinant Human EMG1 His Protein

SDS-Page: EMG1 Recombinant Protein [NBP1-98914]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human EMG1 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-244 of Human EMG1
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAAPSDG FKPRERSGGE QAQDWDALPP KRPRLGAGNK IGGRRLIVVL EGASLETVKV GKTYELLNCD KHKSILLKNG RDPGEARPDI THQSLLMLMD SPLNRAGLLQ VYIHTQKNVL IEVNPQTRIP RTFDRFCGLM VQLLHKLSVR AADGPQKLLK VIKNPVSDHF PVGCMKVGTS FSIPVVSDVR ELVPSSDPIV FVVGAFAHGK VSVEYTEKMV SISNYPLSAA LTCAKLTTAF EEVWGVI

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: EMG1
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 29.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.1 M NaCl, 10% glycerol, 1 mM DTT
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human EMG1 His Protein

• 18S rRNA (pseudouridine-N1-)-methyltransferase NEP1
• BWCNS
• C2F18S rRNA Psi1248 methyltransferase
• EMG1 nucleolar protein homolog (S. cerevisiae)
• essential for mitotic growth 1
• FLJ60792
• Grcc2f
• NEP1EC 2.1.1.-
• Nucleolar protein EMG1 homolog
• probable ribosome biogenesis protein NEP1
• Protein C2f
• Ribosome biogenesis protein NEP1

Background

EMG1 nucleolar protein homolog, also known as EMG1, is a 244 amino acid protein that is thought to be involved in ribosome biogenesis. Localized to the nucleolus, EMG1 participates in pre-18S rRNA processing and may play an important role in the assembly of the small ribosomal subunit, possibly controlling methylation during ribosome synthesis. A mutation in this gene has been associated with Bowen-Conradi syndrome. Recombinant human EMG1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: EMG1