Recombinant Human Endostatin Protein

SDS-Page: Recombinant Human Endostatin Protein [NBP2-34974]

• Reactivity: Hu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Human Endostatin Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 183 amino acids corresponding to the C-terminal fragment of Endostatin Source: E. coli

  Uniprot ID: P39060

  Amino Acid Sequence: HSHRDFQPVL HLVALNSPLS GGMRGIRGAD FQCFQQARAV GLAGTFRAFL SSRLQDLYSI VRRADRAAVP IVNLKDELLF PSWEALFSGS EGPLKPGARI FSFDGKDVLR HPTWPQKSVW HGSDPNGRRL TESYCETWRT EAPSATGQAS SLLGGRLLGQ SAASCHHAYI VLCIENSFMT ASK

• Details of Functionality: Endostatin protein is fully biologically active when compared to standard. The ED50 as determined by inhibiting the FGF basic-dependent proliferation of HUVEC migration is less than 2.0 ug/mL in the presence of Anti-Human Endostatin Polyclonal Antibody.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: COL18A1
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of Endostatin as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 20.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Human Endostatin Protein

• antiangiogenic agent
• COL18A1
• collagen alpha-1(XVIII) chain
• collagen, type XVIII, alpha 1
• Endostatin
• FLJ27325
• FLJ34914
• human type XVIII collagen10endostatin
• KNO
• KNO1MGC74745
• Knobloch syndrome, type 1
• KS
• multi-functional protein MFP

Background

Endostatin is a proteolytic fragment of type XVIII collagen. It inhibits endothelial cell growth, angiogenesis, and wound healing. It also induces the production of the vasorelaxant nitric oxide by endothelial and smooth muscle cells. These actions contribute to the ability of endostatin to block the progression of a wide variety of tumors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: COL18A1
• Uniprot:
  • P39059()