Endotoxin Removal Kit

Product Discontinued

Endotoxin Removal Kit Summary

• Description: This kit can quickly and effectively eliminate endotoxins to <0.05 EU/ml in solutions containing proteins or pharmacologically important components via the immobilized polymyxin B, which is known for capturing endotoxin and preventing toxic effects. High Binding Capacity: up to 9 x 10^8 EU/ml resin.
  High Sample Recovery: >90% recovery with protein solution samples.
  Column content: 5 x 100 ul Rapid Endotoxin Removal Agarose.
  Resin content: Affinity matrix of polymyxin B, supplied as 50% slurry in 20% ethanol.
  Each column can be reused up to 5 times.
  Only requires 5 minutes for each removal cycle.
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab239707)
• Kit Type: Removal Kit

Applications/Dilutions

Publications

Read Publications using NBP2-49796.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.

Background

Endotoxin is the lipopolysaccharide (LPS) complex located in the outer membrane of gram-negative bacteria. A single E.coli bacterium contains ~2 million LPS molecules (2 - 20 fg/cell). During experimental procedures, large amounts of endotoxins are shed and can easily contaminate labware, buffers and downstream products. In vitro, endotoxin causes a variety of problems in cell-based research. In vivo, endotoxin may cause various side effects, including inflammatory response, organ failure or septic shock in host organisms. Therefore, it is critical to remove endotoxin from samples and products.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for Endotoxin Kit (NBP2-49796)(2)

Publications using NBP2-49796

• Cao S, Maulloo C, Raczy M et al. Glycopolymer-conjugated antigens as an inverse vaccine platform prevent anaphylaxis in a pre-clinical model of food allergy bioRxiv 2023-03-25
  
  Details:
  ?-Lactoglobulin (BLG) from bovine milk (certified reference material, Cerilliant®) was purchased from Sigma and purified with an endotoxin removal kit
• Shijie Cao, Chitavi D. Maulloo, Michal M. Raczy, Matthew Sabados, Anna J. Slezak, Mindy Nguyen, Ani Solanki, Rachel P. Wallace, Ha-Na Shim, D. Scott Wilson, Jeffrey A. Hubbell Glycosylation-modified antigens as a tolerance-inducing vaccine platform prevent anaphylaxis in a pre-clinical model of food allergy Cell Reports Medicine 2023-12-20 [PMID: 38128531]