Endotoxin Removal Kit Summary
Description |
High Binding Capacity: Up to 1.5 x 10^9 EU/ml resin. High Sample Recovery: >97% recovery with protein solution samples. Column Content: 5 x 100 ul High Capacity Endotoxin Removal Agarose. Resin Content: affinity matrix of poly-epsilon-lysine, supplied as 50% slurry in 20% ethanol. Each column can be reused up to 5 times (The buffers provided in the kit are sufficient for single use). Buffers are nontoxic. |
Kit Type |
Removal Kit |
Applications/Dilutions
Packaging, Storage & Formulations
Kit Components
Components
|
- Endotoxin removal equilibration buffer
- Endotoxin-free collection tube
- Endotoxin-free water
- High capacity endotoxin removal regeneration buffer
- High capacity endotoxin removal spin column
- High capacity endotoxin removal wash buffer
|
Background
Endotoxin is the lipopolysaccharide (LPS) complex located in the outer membrane of gram-negative bacteria. A single E.coli bacterium contains ~2 million LPS molecules. During experimental procedures, large amounts of endotoxins are shed and can easily contaminate labware, buffers and downstream products. In vitro, endotoxin causes a variety of problems in cell-based research. In vivo, endotoxin may cause various side effects, including inflammatory response, organ failure or septic shock in host organisms. Therefore, it is critical to remove endotoxin from samples and products.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are
guaranteed for 6 months from date of receipt.
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