Eosinophil Antibody (BMK-13)

Flow Cytometry: Mouse Monoclonal Eosinophil Antibody (BMK-13) [NBP1-42140] - LPS stimulated eosinophil. WBC was blocked (RBC lysed whole blood) with 5 % BSA for 15 min and then incubated with NBP1-42140 (1:100) for 2 h at 4 °C. The cells were washed and stained secondary antibody Alexa 430 and analyzed in FACS. Image from a verified customer review.

• Reactivity: Hu, Mu, Ca, Pm, RM
• Applications: ELISA, Flow, IHC
• Clone: BMK-13
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.1 mg/ml

Eosinophil Antibody (BMK-13) Summary

• Immunogen: The antibody reacts with the Eosinophil Major Basic Protein antigen.
• Specificity: Cross-reacts weakly with human basophils, which also contain low level of this protein. It does not cross-react with any other human protein or cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry Validated for Flow from a verified customer review.
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
• Application Notes: This mAb is a very useful tool for clinical and experimental allergy studies. BMK-13 is a pan-eosinophil marker in both frozen sections and paraffin embedded tissues. It stains in bronchial sections 98% of the periphere blood eosinophils. Further, the antibody is most useful for immunohistochemistry on frozen sections is the APAAP method. BMK-13 also binds to eosinophils in paraffin-embedded tissue. A 1:30 dilution in PBS is recommended. Incubation time: 1 hour at RT. Enzymatic pretreatment is necessary. This antibody is suitable for blood smears (cytospins) if fixed with methanol-acetone mixture (1:1, v/v). It is unlikely, without permeabilization, that the BMK-13 is suitable for the flow cytometry because BMK-13 stains intracellular, stored proteins.

Reactivity Notes

Use in Rhesus Macaque reported in scientific literature (PMID:32128254). Canine reactivity reported in scientific literature (PMID: 29920692). Monkey reactivity reported in scientific literature (PMID: 30626745). Mouse reactivity reported in scientific literature (PMID: 31643033, 31626785).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS, 0.1% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Eosinophil Antibody (BMK-13)

• BMPG
• Bone marrow proteoglycan
• EMBP
• Eosinophil granule major basic protein
• Eosinophil major basic protein
• MBP
• MGC14537
• MPG
• Natural killer cell activator
• Pregnancy associated major basic protein
• PRG 2
• Proteoglycan 2 bone marrow
• Proteoglycan 2 preproprotein
• Proteoglycan 2
• Proteoglycan2

Background

PRG2 is a cytotoxin and helminthotoxin. Also induces noncytolytic histamine release from human basophils. Involved in antiparasitic defense mechanisms and immune hypersensitivity reactions. The proform acts as a proteinase inhibitor, reducing the activity of PAPPA. In pregnancy serum, the proform exists as a disulfide-linked 2:2 heterotetramer with PAPPA, as a disulfide-linked 2:2 heterotetramer with AGT, and as a complex (probably a 2:2:2 heterohexamer) with AGT and C3dg.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Eosinophil Antibody (NBP1-42140)(7)

Publications using NBP1-42140

• Dinesh Devadoss, Kerri Surbaugh, Marko Manevski, Chatura Wickramaratne, Dale Chaput, Arianne Chung, Francisco de Leon, Hitendra S Chand, Jaspreet S Dhau Indoor-air purification by photoelectrochemical oxidation mitigates allergic airway responses to aerosolized cat dander in a murine model. Scientific reports 2023-07-10 [PMID: 37414804]
• Roy C J, Van Slyke G et al. Passive immunization with an extended half-life monoclonal antibody protects Rhesus macaques against aerosolized ricin toxin. NPJ Vaccines 2020-05-03 [PMID: 32128254] (IF/IHC, Rhesus Macaque)
• Singh SP, Chand HS, Banerjee S et al. Acetylcholinesterase Inhibitor Pyridostigmine Bromide Attenuates Gut Pathology and Bacterial Dysbiosis in a Murine Model of Ulcerative Colitis Dig. Dis. Sci. 2019-10-23 [PMID: 31643033] (IF/IHC, Mouse)
• Hashimoto T, Kursewicz CD, Fayne RA et al. Mechanisms of itch in stasis dermatitis: Significant role of IL-31 from macrophages J. Invest. Dermatol. 2019-10-15 [PMID: 31626785] (Mouse)
• Hashimoto T, Kursewicz CD, Fayne RA et al. Pathophysiological mechanisms of itch in bullous pemphigoid J. Am. Acad. Dermatol. 2019-07-25 [PMID: 31351883]
• Roy CJ, Ehrbar DJ, Bohorova N et al. Rescue of rhesus macaques from the lethality of aerosolized ricin toxin. JCI Insight 2019-01-10 [PMID: 30626745] (IHC-P, Monkey)
• Costa SF, Trivellato GF, Rebech GT et al. Eosinophilic inflammation in lymph nodes of dogs with visceral leishmaniasis Parasite Immunol. 2018-06-19 [PMID: 29920692] (ELISA, Canine)

Review for Eosinophil Antibody (NBP1-42140) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer Flow Mouse 03/21/2025

Summary

• Application: Flow Cytometry
• Sample Tested: Whole blood
• Species: Mouse
• Lot: 186008L

Comments

• Comments: ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.***WBC was blocked (RBC lysed whole blood) with 5 % BSA for 15 min and then incubated with NBP1-42140 (1:100) for 2 h at 4 °C. The cells were washed and stained secondary antibody Alexa 430 and analyzed in FACS.

FAQs for Eosinophil Antibody (NBP1-42140). (Showing 1 - 1 of 1 FAQs).

1. I would appreciate it very much if you could let me know the ingredients of the buffer this antibody is stored in?
   • This antibody is supplied as 1 ml purified antibody (conc. 100 ug/ml) in PBS + 0.1% BSA + 0.02% sodium azide.