EpCAM/TROP1 Antibody [Biotin]

HT-29 cells were stained with Goat Anti-Human EpCAM/TROP‑1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF960, filled histogram) or isotype control antibody (Catalog # BAF108, open histogram) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: WB, Flow, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

EpCAM/TROP1 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human EpCAM, extracelllular domain
  Gln24-Lys265
  Accession # CAA32870
• Specificity: Detects human EpCAM/TROP-1 in sandwich immunoassays and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human (rh) ALCAM, rhBCAM, recombinant mouse MAdCAM-1, rhNCAM-1, and rhNCAM-L1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: EPCAM
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Human EpCAM/TROP-1 Antibody (Catalog # MAB9601)
  ELISA Detection: Human EpCAM/TROP-1 Biotinylated Antibody (Catalog # BAF960)
  Standard: Recombinant Human EpCAM/TROP-1 Fc Chimera (Catalog # 960-EP)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EpCAM/TROP1 Antibody [Biotin]

• 17-1A
• 323/A3
• ACSTD1
• antigen identified by monoclonal AUA1
• CD326 antigen
• CD326
• Cell surface glycoprotein Trop-1
• chromosome 4, surface marker (35kD glycoprotein)
• DIAR5
• EGP
• EGP-2
• EGP314
• EGP40
• EpCAM
• epithelial cell adhesion molecule
• Epithelial cell surface antigen
• Epithelial glycoprotein 314
• Epithelial glycoprotein
• ESA
• GA733-2
• GA733-2EGP34
• gp40
• hEGP314
• HNPCC8
• KS 1/4 antigen
• KS1/4
• KSAHEA125
• M1S2
• M4S1
• M4S1Ly74
• Major gastrointestinal tumor-associated protein GA733-2
• MIC18MH99
• MOC31
• TACST-1
• TACSTD1
• TROP1
• TROP1CD326
• Tumor-associated calcium signal transducer 1CO-17A

Background

Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP-1, is a 40 kDa transmembrane glycoprotein composed of a 242 amino acid (aa) extracellular domain with two epidermal-growth-factor-like (EGF-like) repeats within the cysteine-rich N-terminal region, a 23 aa transmembrane domain, and a 26 aa cytoplasmic domain. Human and mouse EpCAM share 82% aa sequence identity. In human, EpCAM also shares 49% aa sequence homology with TROP-2/EGP-1. During embryonic development, EpCAM is detected in fetal lung, kidney, liver, pancreas, skin, and germ cells. In adults, human EpCAM is detected in basolateral cell membranes of all simple, pseudo-stratified, and transitional epithelia, but is not detected in normal squamous stratified epithelia, mesenchymal tissue, muscular tissue, neuro-endocrine tissue, or lymphoid tissue (1). EpCAM expression has been found to increase in actively proliferating epithelia tissues and during adult liver regeneration (1, 2). EpCAM expression is also found to increase in human malignant neoplasias, with most carcinoma expressing EpCAM including those of arising from squamousal epithelia (1). EpCAM has been shown function as a homophilic Ca²⁺ independent adhesion molecule (3). Homophilic adhesion via EpCAM requires the interaction of both EGF-like repeats, with the first EGF-like repeat mediating reciprocal interaction between EpCAM molecules on opposing cells, while the second repeat is involved in lateral interaction of EpCAM. Lateral interaction of EpCAM lead to the formation of dimers and tetramers (4). During homophilic adhesion the cytoplasmic tail of EpCAM interacts with the actin cytoskeleton via a direct association alpha -actinin (5).

1. Balzar, M. et al. (1999) J. Mol. Med. 77:699.
2. Boer, C.J, et al. (1999) J. Pathol. 188:201.
3. Litvinow, S.V. et al. (1994) J. Cell Biol. 125:437.
4. Balzar, M. et al. (2001) Mol. Cell. Biol. 21:2570.
5. Balzar, M. et al. (1998) Mol. Cell. Biol. 18:4388.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EpCAM/TROP1 Antibody (BAF960)(28)

Publications using BAF960

• Xiaoqiang Wang, Song Zhang, Di Jin, Jiamei Luo, Yumiao Shi, Yiqi Zhang et al. μ‐opioid receptor agonist facilitates circulating tumor cell formation in bladder cancer via the MOR/AKT/Slug pathway: a comprehensive study including randomized controlled trial Cancer Communications 3/1/2023 [PMID: 36739595]
• Q Niu, J Gao, K Zhao, X Chen, X Lin, C Huang, Y An, X Xiao, Q Wu, L Cui, P Zhang, L Wu, C Yang Fluid nanoporous microinterface enables multiscale-enhanced affinity interaction for tumor-derived extracellular vesicle detection Proceedings of the National Academy of Sciences of the United States of America, 2022-10-28;119(44):e2213236119. 10/28/2022 [PMID: 36306324]
• L Xu, T Ma, K Zhang, Q Zhang, M Yu, X Zhao Multifunctional Gelatin-Nanoparticle-Modified Chip for Enhanced Capture and Non-Destructive Release of Circulating Tumor Cells Micromachines, 2022-02-28;13(3):. 2022-02-28 [PMID: 35334686] (ELISA Development, Human)
• H Shen, R Su, J Peng, L Zhu, K Deng, Q Niu, Y Song, L Yang, L Wu, Z Zhu, C Yang Antibody-engineered red blood cell interface for high-performance capture and release of circulating tumor cells Bioactive materials, 2021-10-05;11(0):32-40. 2021-10-05 [PMID: 34938910] (Cell Capture, Human)
• C Liu, H Chen, T Sun, H Wang, B Chen, X Wang The value of circulating tumor cells with positive centromere probe 8 in the diagnosis of small pulmonary nodules Translational Oncology, 2021-03-02;14(5):101052. 2021-03-02 [PMID: 33667891] (IHC, Human)
• O Gur, CL Chang, R Jain, Y Zhong, CA Savran High-purity isolation of rare single cells from blood using a tiered microchip system PLoS ONE, 2020-03-17;15(3):e0229949. 2020-03-17 [PMID: 32182245] (ELISA Capture, Human)
• M Zeinali, M Lee, A Nadhan, A Mathur, C Hedman, E Lin, R Harouaka, MS Wicha, L Zhao, N Palanisamy, M Hafner, R Reddy, GP Kalemkeria, BJ Schneider, KA Hassan, N Ramnath, S Nagrath High-Throughput Label-Free Isolation of Heterogeneous Circulating Tumor Cells and CTC Clusters from Non-Small-Cell Lung Cancer Patients Cancers (Basel), 2020-01-03;12(1):. 2020-01-03 [PMID: 31947893] (ICC, Human)
• TH Kim, Y Wang, CR Oliver, DH Thamm, L Cooling, C Paoletti, KJ Smith, S Nagrath, DF Hayes A temporary indwelling intravascular aphaeretic system for in vivo enrichment of circulating tumor cells Nat Commun, 2019-04-01;10(1):1478. 2019-04-01 [PMID: 30932020] (Cell Capture, Xenograft)
• M Reis-Sobre, JF Chen, T Novitskaya, S You, S Morley, K Steadman, N Kaur Gill, A Eskaros, M Rotinen, CY Chu, LWK Chung, H Tanaka, W Yang, BS Knudsen, HR Tseng, AC Rowat, EM Posadas, A Zijlstra, D Di Vizio, MR Freeman Emerin deregulation links nuclear shape instability to metastatic potential Cancer Res., 2018-08-28;0(0):. 2018-08-28 [PMID: 30154147] (Cell Capture, Human)
• YH Wang, J Ji, BC Wang, H Chen, ZH Yang, K Wang, CL Luo, WW Zhang, FB Wang, XL Zhang Tumor-Derived Exosomal Long Noncoding RNAs as Promising Diagnostic Biomarkers for Prostate Cancer Cell. Physiol. Biochem., 2018-03-26;46(2):532-545. 2018-03-26 [PMID: 29614511] (Functional Assay, Human)
• Y Hao, H Liu, G Li, H Cui, L Jiang, S Wang Photo and Thermo Dual-Responsive Copolymer Surfaces for Efficient Cell Capture and Release Chemphyschem, 2018-04-14;0(0):. 2018-04-14 [PMID: 29543403] (ICC, Human)
• H Lee, M Choi, J Lim, M Jo, JY Han, TM Kim, Y Cho Magnetic Nanowire Networks for Dual-Isolation and Detection of Tumor-Associated Circulating Biomarkers Theranostics, 2018-01-01;8(2):505-517. 2018-01-01 [PMID: 29290823] (Functional Assay)
• MH Park, E Reátegui, W Li, SN Tessier, KH Wong, AE Jensen, V Thapar, D Ting, M Toner, SL Stott, PT Hammond Enhanced Isolation and Release of Circulating Tumor Cells Using Nanoparticle Binding and Ligand Exchange in a Microfluidic Chip J. Am. Chem. Soc., 2017-02-09;139(7):2741-2749. 2017-02-09 [PMID: 28133963] (Cell Selection, N/A)
• Increased expression of long-noncoding RNA ZFAS1 is associated with epithelial mesenchymal transition of gastric cancer Aging (Albany NY), 2016-09-18;0(0):. 2016-09-18 [PMID: 27654478] (ELISA Development (Detection), Human)
• Daniel J. Joe, Jeonghyun Hwang, Christelle Johnson, Ho-Young Cha, Jo-Won Lee, Xiling Shen et al. Surface Functionalized Graphene Biosensor on Sapphire for Cancer Cell Detection Journal of Nanoscience and Nanotechnology 7/12/2016 [PMID: 27398439]
• J S Ankeny Circulating tumour cells as a biomarker for diagnosis and staging in pancreatic cancer Br J Cancer, 2016-06-14;114(12):1367-75. 2016-06-14 [PMID: 27300108] (NanoVelcro Chip, Human)
• Mei-Ying Liao, Jun-Kai Lai, Mark Yen-Ping Kuo, Ruei-Min Lu, Cheng-Wei Lin, Ping-Chang Cheng et al. An anti-EpCAM antibody EpAb2-6 for the treatment of colon cancer Oncotarget 9/22/2015 [PMID: 26317650]
• Seeber A, Martowicz A, Spizzo G, Buratti T, Obrist P, Fong D, Gastl G, Untergasser G Soluble EpCAM levels in ascites correlate with positive cytology and neutralize catumaxomab activity in vitro. BMC Cancer, 2015-05-07;15(0):372. 2015-05-07 [PMID: 25947366] (ELISA Development (Detection), Human)
• Sarah Javaid, Jianmin Zhang, Gromoslaw A. Smolen, Min Yu, Ben S. Wittner, Anurag Singh et al. MAPK7 Regulates EMT Features and Modulates the Generation of CTCs Molecular Cancer Research 5/1/2015 [PMID: 25678598]
• Bryan , R T, Shimwell , N J, Wei , W, Devall , A J, Pirrie , S J, James , N D, Zeegers , M P, Cheng , K K, Martin , A, Ward , D G Urinary EpCAM in urothelial bladder cancer patients: characterisation and evaluation of biomarker potential. Br J Cancer, 2013-11-28;110(3):679-85. 2013-11-28 [PMID: 24292452] (Immunoprecipitation, Western Blot, Human)
• Grace D. Chen, Fabio Fachin, Elena Colombini, Brian L. Wardle, Mehmet Toner Nanoporous micro-element arrays for particle interception in microfluidic cell separation Lab on a Chip 9/7/2012 [PMID: 22763858]
• Yu M, Ting DT, Stott SL et al. RNA sequencing of pancreatic circulating tumour cells implicates WNT signalling in metastasis. Nature 7/1/2012 [PMID: 22763454]
• Ajay M. Shah, Min Yu, Zev Nakamura, Jordan Ciciliano, Matthew Ulman, Kenneth Kotz et al. A Biopolymer System for Cell Recovery from Microfluidic Cell Capture Devices Analytical Chemistry 4/17/2012 [PMID: 22414137]
• Tania Konry, Irina Smolina, Joel M. Yarmush, Daniel Irimia, Martin L. Yarmush Ultrasensitive Detection of Low-Abundance Surface-Marker Protein Using Isothermal Rolling Circle Amplification in a Microfluidic Nanoliter Platform Small 2/7/2011 [PMID: 21294269]
• Punnoose EA, Atwal SK, Spoerke JM Molecular biomarker analyses using circulating tumor cells. PLoS ONE, 2010-09-08;5(9):e12517. 2010-09-08 [PMID: 20838621] (Flow Cytometry, Human)
• Helzer KT, Barnes HE, Day L, Harvey J, Billings PR, Forsyth A Circulating tumor cells are transcriptionally similar to the primary tumor in a murine prostate model. Cancer Res., 2009-09-29;69(19):7860-6. 2009-09-29 [PMID: 19789350] (Immunoprecipitation, Human)
• Burger PE, Gupta R, Xiong X, Ontiveros CS, Salm SN, Moscatelli D, Wilson EL High aldehyde dehydrogenase activity: a novel functional marker of murine prostate stem/progenitor cells. Stem Cells, 2009-09-01;27(9):2220-8. 2009-09-01 [PMID: 19544409] (Flow Cytometry, Mouse)
• Nagrath S, Sequist LV, Maheswaran S, Bell DW, Irimia D, Ulkus L, Smith MR, Kwak EL, Digumarthy S, Muzikansky A, Ryan P, Balis UJ, Tompkins RG, Haber DA, Toner M Isolation of rare circulating tumour cells in cancer patients by microchip technology. Nature, 2007-12-20;450(7173):1235-9. 2007-12-20 [PMID: 18097410] (Human)

FAQs for EpCAM/TROP1 Antibody (BAF960). (Showing 1 - 1 of 1 FAQs).

1. I'm looking for EpCAM antibody for rat. Do you have any antibody which can use FACS?
   • We unfortunately do not have any EpCAM antibodies validated in FACS for detection of the rat protein. I am sorry for any inconvenience. I do have three products to EpCAM that can detect the rat protein. These products have been validated to detect the native rat protein, so they may work for FACS however they have not yet been tested in that particular application. If you would like to try them in your experiment you would qualify for our Innovator's Reward Program. This program was designed to help scientists with the cost of their antibodies in return for valuable information. If you decide to test an antibody in a previously untested species or application we will issue you a credit for the purchase price of the antibody in exchange for a review of your experiment using our product.

Bioinformatics

• Gene Symbol: EPCAM
• Uniprot:
  • CAA32870()