Recombinant Human EPGN Protein

SDS-Page: Human EPGN Protein [NBP2-34987]

• Reactivity: Hu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Human EPGN Protein Summary

• Description: A monomeric protein containing 72 amino acid residues, which comprises the EGF homologous portion of the Epigen precursor Source: E. coli

  Uniprot ID: Q6UW88

  Amino Acid Sequence: AVTVTPPITA QQADNIEGPI ALKFSHLCLE DHNSYCINGA CAFHHELEKA ICRCFTGYTG ERCEHLTLTS YA

• Details of Functionality: EPGN protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 300 ng/ml, corresponding to a specific activity of > 3.3 x 10^3 IU/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: EPGN
• Purity: > 98 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of EPGN as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 7.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: > 98 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Human EPGN Protein

• epigen
• epithelial mitogen homolog (mouse)
• PRO9904
• UNQ3072/PRO9904

Background

Epigen is a member of the epidermal growth factor family and is expressed by several tissues, including the testis, liver, heart and in certain tumor cells. It is strongly mitogenic for fibroblasts and epithelial cells, despite its relatively weak affinity for its main receptor, ErbB1. The mitogenic potential of Epigen is enhanced by its unusually long persistence on the membrane before ubiquitylation and receptor-mediated depletion. The gene of human Epigen encodes a 154 amino acid (a.a.) residue type I transmembrane precursor glycoprotein which contains a 22 a.a. signal peptide, an 88 a.a. extracellular domain, a 21 a.a. transmembrane domain and a 23 a.a. cytoplasmic domain. By alternative splicing of the gene, Epigen has several isoforms. The mature, shed form of human Epigen (54 - 104a.a.) shares 92 %, 94 % and 94 % a.a. sequence identity with mouse, rat and equine Epigen, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: EPGN
• Uniprot:
  • Q6UW88()
  • Q6UW88()
  • Q6UW88()