ErbB2/Her2 Antibody (191924) [Phycoerythrin]

MCF-7 human breast cancer cell line was stained with Mouse Anti-Human ErbB2/Her2 PE-conjugated Monoclonal Antibody (Catalog # FAB1129P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

MDA-MB-453 human breast cancer cell line was stained with Mouse Anti-Human ErbB2/Her2 PE-conjugated Monoclonal Antibody (Catalog # FAB1129P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 191924
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

ErbB2/Her2 Antibody (191924) [Phycoerythrin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ErbB2/Her2
  Thr23-Thr652
  Accession # P04626
• Specificity: Detects human ErbB2/Her2 in ELISAs and Western blots. In ELISAs and Western blots, this antibody does not cross-react with recombinant human (rh) EGF R, rhErbB3, or rhErbB4.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ERBB2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ErbB2/Her2 Antibody (191924) [Phycoerythrin]

• CD340 antigen
• CD340
• c-erb B2/neu protein
• EC 2.7.10
• EGFR2
• ErbB2
• HER2
• HER-2
• HER2EC 2.7.10.1
• herstatin
• Metastatic lymph node gene 19 protein
• MLN 19
• MLN19
• Neu Oncogene
• NEUHER-2/neu
• neuroblastoma/glioblastoma derived oncogene homolog
• NGL
• NGLTKR1
• p185erbB2
• Proto-oncogene c-ErbB-2
• Proto-oncogene Neu
• receptor tyrosine-protein kinase erbB-2
• TKR1
• Tyrosine kinase-type cell surface receptor HER2
• v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog)
• v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian)

Background

ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. ErbB2 is widely expressed in epithelial cells and has also been found to be over-expressed in a large number of breast carcinomas. Among ErbB family members, ErbB2 is unique in that it has no identified ligands. Rather, ErbB2 heterodimerizes with the other members of the ErbB family (ErbB1 (EGF R), ErbB3, ErbB4) to form higher affinity signaling complexes. Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor. It has been found that a discrete three amino acid signal in the ErbB3 cytoplasmic domain is critical for transactivation of ErbB2. Interestingly, this same three amino acid signal has also been found in ErbB1 and ErbB4. Phosphoinositide 3-kinase has been shown to play a role in ErbB2 signal transduction. The cytoplasmic domain of ErbB2 has been shown to associate with beta-catenin and plakoglobin. Human ErbB2 consists of 1255 amino acids (aa) with a 21 aa signal sequence, a 631 aa extracellular domain, a 23 aa transmembrane region, and a 580 aa cytoplasmic domain. ErbB2 can be shed from the cell surface by proteolytic cleavage by an unidentified protease. ErbB2 appears to play roles in development, cancer, communication at the neuromuscular junction, and regulation of cell growth and differentiation (1-10).

1. Coussens, L. et. al. (1985) Science 230:1132.
2. Yamamoto, T. et. al. (1986) Nature 319:230.
3. Kanai, Y. et. al. (1995) Biochem. Biophys. Res. Commun. 208:1067.
4. Codony-Servat, J. et. al. (1999) Cancer Res. 59:1196.
5. Carraway, K.L. 3^rd et. al.  (1994) J. Biol. Chem. 269:14303.
6. Emkey, R. and C.R. Kahn (1997) J. Biol. Chem. 272:31172.
7. Schaefer, G. et. al. (1999) J. Biol. Chem. 274:859.
8. Schlessinger, J. (2000) Cell 103:211.
9. Hellyer, N.J. et. al. (2001) J. Biol. Chem. 276:42153.
10. Daly, R.J. (1999) Growth Factors 16:255.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ErbB2/Her2 Antibody (FAB1129P)(10)

Publications using FAB1129P

• JT de Kay, J Carver, B Shevenell, AM Kosta, S Tsibulniko, E Certo, DB Sawyer, S Ryzhov, MP Robich Decreased expression of ErbB2 on left ventricular epicardial cells in patients with diabetes mellitus Cellular Signalling, 2022-05-21;96(0):110360. 2022-05-21 [PMID: 35609807] (Flow Cytometry, Human)
• I De Pauw, F Lardon, J Van den Bo, H Baysal, E Fransen, V Deschoolme, P Pauwels, M Peeters, JB Vermorken, A Wouters Simultaneous targeting of EGFR, HER2 and HER4 by afatinib overcomes intrinsic and acquired cetuximab resistance in head and neck squamous cell carcinoma cell lines Mol Oncol, 2018-05-01;0(0):. 2018-05-01 [PMID: 29603584] (Flow Cytometry, Human)
• Bielamowicz K, Fousek K, Byrd T, Samaha H, Mukherjee M, Aware N, Wu M, Orange J, Sumazin P, Man T, Joseph S, Hegde M, Ahmed N Trivalent CAR T cells overcome interpatient antigenic variability in glioblastoma. Neuro Oncol, 2018-03-27;20(4):506-518. 2018-03-27 [PMID: 29016929] (CAR-T (Flow Cytometry), Human)
• S Kéri, C Szabó, O Kelemen Uniting the neuro developmental and immunological hypotheses: Neuregulin 1 receptor ErbB and Toll-like receptor activation in first-episode schizophrenia Sci Rep, 2017-06-23;7(1):4147. 6/23/2017 [PMID: 28646138]
• Sergey Ryzhov, Anton Matafonov, Cristi L. Galindo, Qinkun Zhang, Truc-Linh Tran, Daniel J. Lenihan et al. ERBB signaling attenuates proinflammatory activation of nonclassical monocytes American Journal of Physiology-Heart and Circulatory Physiology 2017-05-01 [PMID: 28235789] (Flow Cytometry, Human)
• Hirotaka Kanzaki, Nishit K. Mukhopadhya, Xiaojiang Cui, V. Krishnan Ramanujan, Ramachandran Murali Trastuzumab-Resistant Luminal B Breast Cancer Cells Show Basal-Like Cell Growth Features Through NF-kappa B-Activation Monoclonal Antibodies in Immunodiagnosis and Immunotherapy 2/13/2016 [PMID: 26871511]
• Sie M, den Dunnen W, Lourens H, Meeuwsen-de Boer T, Scherpen F, Zomerman W, Kampen K, Hoving E, de Bont E Growth-factor-driven rescue to receptor tyrosine kinase (RTK) inhibitors through Akt and Erk phosphorylation in pediatric low grade astrocytoma and ependymoma. PLoS ONE, 2015-03-23;10(3):e0122555. 2015-03-23 [PMID: 25799134] (Flow Cytometry, Human)
• Mirkina I, Hadzijusufovic E, Krepler C, Mikula M, Mechtcheriakova D, Strommer S, Stella A, Jensen-Jarolim E, Holler C, Wacheck V, Pehamberger H, Valent P Phenotyping of human melanoma cells reveals a unique composition of receptor targets and a subpopulation co-expressing ErbB4, EPO-R and NGF-R. PLoS ONE, 2014-01-29;9(1):e84417. 2014-01-29 [PMID: 24489649] (Flow Cytometry, Human)
• Nakanishi T, Chumsri S, Khakpour N, Brodie AH, Leyland-Jones B, Hamburger AW, Ross DD, Burger AM Side-population cells in luminal-type breast cancer have tumour-initiating cell properties, and are regulated by HER2 expression and signalling. Br. J. Cancer, 2010-02-09;102(5):815-26. 2010-02-09 [PMID: 20145614] (Flow Cytometry, Human)
• Dai CL, Tiwari AK, Wu CP, Su XD, Wang SR, Liu DG, Ashby CR, Huang Y, Robey RW, Liang YJ, Chen LM, Shi CJ, Ambudkar SV, Chen ZS, Fu LW Lapatinib (Tykerb, GW572016) reverses multidrug resistance in cancer cells by inhibiting the activity of ATP-binding cassette subfamily B member 1 and G member 2. Cancer Res., 2008-10-01;68(19):7905-14. 2008-10-01 [PMID: 18829547] (Flow Cytometry, Human)

FAQs for ErbB2/Her2 Antibody (FAB1129P). (Showing 1 - 1 of 1 FAQs).

1. I am interested in getting antibodies for HER that would work in flow cytometry. Could you recommend some choices?
   • A list of our ErbB2 antibodies validated for use in flow cytometry can be found here /productsearch/HER2#fq=common_name%3A%22ErbB2%2FHER2%22&fq=category%3A%22Primary%20Antibodies%22&fq=applications%3A%22Flow%20Cytometry%22

Bioinformatics

• Gene Symbol: ERBB2
• Uniprot:
  • P04626()