Human ErbB2/Her2 Luciferase - (LUCPorter™) Stable Reporter Cell Line

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Human ErbB2/Her2 Luciferase - (LUCPorter™) Stable Reporter Cell Line Summary

• Description: The HER2/LUCPorter reporter cell line is designed to monitor the induction of HER2 and can be used for screening of agonists, antagonists or signaling inhibitors of HER2 induction as well as for studying the HER2 induction-related signaling pathways.
  
  Contents : 3 ~ 4 x 10^6 cells
  Biosafety Level : 2
• Immunogen: The HER2/LUCPorter reporter cell line is a stably transfected HEK 293 cell line which expresses an optimized Renilla luciferase reporter gene (RenSP) under the transcriptional control of the HER2 promoter.
• Target Species: Human
• Selection Agent: Puromycin
• Growth Properties: Adherent
  Morphology : Epithelial
• RCL Type: Luciferase - (LUCPorter™)
• Host: HEK293
• Gene: ERBB2

Applications/Dilutions

• Dilutions:
  • Ligand Activation
• Readout System:
  • LightSwitch Luciferase Assay Reagent NBP2-32066

Packaging, Storage & Formulations

• Storage: Store in gas phase of liquid nitrogen.
• Reconstitution Instructions: Complete Growth Medium
  DMEM with 4.5 g/L glucose + 10% FBS + 4 mM L-glutamine + 1 mM sodium pyruvate + 100 units/ml penicillin + 0.1 mg/ml streptomycin + 3 ug/ml puromycin.

Notes

Safety Considerations
Assume all cultures are hazardous since they may harbor latent viruses or other organisms that are uncharacterized. The following safety precautions should be observed. - Use pipette aids to prevent ingestion and keep aerosols down to a minimum. - No eating, drinking or smoking while handling the reporter line. -Wash hands after handling the reporter cell line and before leaving the lab. - Decontaminate work surface with disinfectant or 70% ethanol before and after working with the reporter line. -All waste should be considered hazardous. - Dispose of all liquid waste after each experiment and treat with bleach.

Alternate Names for Human ErbB2/Her2 Luciferase - (LUCPorter™) Stable Reporter Cell Line

• CD340 antigen
• CD340
• c-erb B2/neu protein
• EC 2.7.10
• EGFR2
• ErbB2
• HER2
• HER-2
• HER2EC 2.7.10.1
• herstatin
• Metastatic lymph node gene 19 protein
• MLN 19
• MLN19
• Neu Oncogene
• NEUHER-2/neu
• neuroblastoma/glioblastoma derived oncogene homolog
• NGL
• NGLTKR1
• p185erbB2
• Proto-oncogene c-ErbB-2
• Proto-oncogene Neu
• receptor tyrosine-protein kinase erbB-2
• TKR1
• Tyrosine kinase-type cell surface receptor HER2
• v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog)
• v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian)

Background

As a member of the epidermal growth factor receptor family, HER2 (human epidermal growth factor receptor 2; also known as Neu, ErbB-2, CD340 or p185) has been known to play an important role in breast cancer development and progression. Dysregulation of HER2 signaling such as excess gene amplification and overexpression may result in deleterious effects of HER2 in tumor cells. Elevation of HER2 is often observed in a high proportion of metastatic breast cancers, enhancing aggressive tumor cell growth. Hence therapeutic inhibition of HER2 in metastatic breast cancers would suppress tumor cell proliferation and provide synergistic effects with other cancer treatments. The HER2/LUCPorter reporter cell line is a semi-constitutively active cell line and also ligand/agonist-inducible cell line as shown in Figures 1 and 2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Reporter Cell Lines are guaranteed for 1 year from date of receipt.

FAQs for ErbB2/Her2 Reporter Cell Line (NBP2-30092). (Showing 1 - 1 of 1 FAQ).

1. I am interested in getting antibodies for HER that would work in flow cytometry. Could you recommend some choices?
   • A list of our ErbB2 antibodies validated for use in flow cytometry can be found here /productsearch/HER2#fq=common_name%3A%22ErbB2%2FHER2%22&fq=category%3A%22Primary%20Antibodies%22&fq=applications%3A%22Flow%20Cytometry%22

Bioinformatics

• Gene Symbol: ERBB2