Human Erythropoietin/EPO ELISA Kit (Colorimetric)

Product Discontinued

Human Erythropoietin/EPO ELISA Kit (Colorimetric) Summary

• Specificity: The assay detects both natural and recombinant human Erythropoietin. The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into serum. There was no crossreactivity detected.
• Standard Curve Range: 1.6 - 100 mIU/mL
• Sensitivity: 0.17 mIU/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 8.5%
• Intra-Assay: CV% < 7.8%
• Spike Recovery: 105.2
• Sample Volume: 50 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: EPO

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: The human Erythropoietin ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human Erythropoietin.

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Erythropoietin/EPO ELISA Kit (Colorimetric)

• ECYT5
• EP
• EPO
• epoetin
• Erythropoietin
• MGC138142
• MVCD2

Background

Erythropoietin (EPO) is a hormone produced by the kidney that promotes the formation of red blood cells in the bone marrow. EPO is a glycoprotein with a molecular weight of 34 kDa. The kidney cells that primarily make EPO are specialized and sensitive to low oxygen levels in the blood. These cells release EPO when the oxygen level is low in the kidney. EPO binds the Epo receptor (Epo R) on erythroid progenitors in bone marrow eliciting proliferation, maturation, and differentiation of red blood cells thereby increase the oxygen-carrying capacity of the blood. EPO is the prime physiological regulator of red blood cell production. The measurement of EPO in the blood is useful in the study of bone marrow disorders and kidney disease. Elevated levels of EPO can be seen in polycythemia, a disorder in which there is an excess of red blood cells. Lower than normal levels of EPO are seen in chronic renal failure. EPO plays an important role in the brain's response to neuronal injury. EPO is also involved in the wound healing process. Measurement of serum immunoreactive erythropoietin has shown that overproduction of EPO can be an adaptive response to conditions producing tissue hypoxia, such as smoking chronic obstructive pulmonary disease, renal hypoxia or cyanotic heart disease. Elevated serum levels have further been found in patients suffering from various neoplastic diseases, such as renal carcinomas and benign renal tumors, liver carcinomas and hepatomas, cerebellar hemangioblastomas and other neoplastic disorders. Serum levels of EPO lower than normal are found in various forms of anemias. In case EPO is not primarily involved in the cause of the anemia, elevated levels of EPO are found in serum of the patients suffering from diseases like aplastic anemias, iron deficiency, megaloblastic anemias, thalassemias and myelodysplastic syndromes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Erythropoietin/EPO ELISA Kit (NBP2-31082)(1)

Publication using NBP2-31082

• Mancera-Soto E, Ramos-Caballero DM, Magalhaes J et al. Quantification of testosterone-dependent erythropoiesis during male puberty Experimental physiology 2021-05-04 [PMID: 33945170] (ELISA)

FAQs for Erythropoietin/EPO ELISA Kit (NBP2-31082). (Showing 1 - 2 of 2 FAQ).

1. Our customer wants to know the origin of cell for erythropoietin in NBP2-31082. Could you let me know?
   • Our EPO ELISA kit with catalogue number NBP2-31082 is designed to be used for the quantitative detection of human Erythropoietin. Cell culture supernatant, serum and plasma (EDTA, citrate, heparin), have all been tested with this assay. Our lab informed me that to test the suitability of this kit for use with cell culture samples, we spiked cell culture medium (RPMI medium) with 10% FCS. The erythropoietin was obtained from recombinant EPO expressed in CHO cells.
2. Could you tell me how many plates are supplied in the NBP2-31082?
   • NBP2-31082 contains one 96 well microtitre plate.

Bioinformatics

• Gene Symbol: EPO