Recombinant Human Erythropoietin/EPO Protein

SDS-Page: Recombinant Human Erythropoietin Protein [NBP2-61250] - Erythropoietin [NBP2-61250] - Recombinant human

Product Discontinued

Recombinant Human Erythropoietin/EPO Protein Summary

• Description: Mature human EPO, containing 166 amino acid residues.
  
  

  Source: CHO cell line

  
  
  AA Sequence: APPRLICDSR VLERYLLEAK EAENITTGCA EHCSLNENIT VPDTKVNFYA WKRMEVGQQA VEVWQGLALL SEAVLRGQAL LVNSSQPWEP LQLHVDKAVS GLRSLTTLLR ALGAQKEAIS PPDAASAAPL RTITADTFRK LFRVYSNFLR GKLKLYTGEA CRTGDR

• Details of Functionality: Fully biologically active when compared to standard. The Specific Activity was measured by the stimulation of reticulocyte production in normocyth-aemic mice and is no less than 1.5 x 105 IU/mg.
• Source: CHO
• Protein/Peptide Type: Recombinant Protein
• Gene: EPO
• Purity: >98%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
• Endotoxin Note: Less than 0.01 EU/ug of rHuEPO-a as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Functional
  • SDS-Page
• Application Notes: Has a predicted molecular mass of approximately 21 kDa. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 36-40 kDa in SDS-PAGE.
• Theoretical MW: 38 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in sodium citrate buffer (1 liter of ddH2O containing 5.9 g of sodium citrate, 5.8 g of sodium chloride and 0.06 g of citric acid).
• Concentration: LYOPH
• Purity: >98%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Alternate Names for Recombinant Human Erythropoietin/EPO Protein

• ECYT5
• EP
• EPO
• epoetin
• Erythropoietin
• MGC138142
• MVCD2

Background

Anti-Erythropoietin is tested for its ability to neutralize the biological activity of recombinant human EPO on the human cell line TF-1. The ND50 of the antibody is defined as the concentration of antibody resulting in a one-half maximal inhibition of bioactivity of recombinant human EPO, when recombinant human EPO is present at a concentration just high enough to elicit a maximum response. Erythropoietin is a humoral factor that stimulates erythropoiesis. EPO has four introns and five exons and contains 165 amino acids. EPO induces growth and differentiation of burst forming units and colony forming units into mature red blood cells. Human erythropoietin stimulates growth of rodent bone marrow cells. Recombinant erythropoietin is produced in CHO cells by a DNA sequence encoding the human EPO protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: EPO