F-Actin Antibody (NH3) - BSA Free

• Reactivity: Hu, Mu, Ch, Rb
• Applications: ELISA, Flow
• Clone: NH3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

F-Actin Antibody (NH3) - BSA Free Summary

• Immunogen: Human monocytes and the human monocyte-like cell line, U937.
• Specificity: The epitope recognized is preserved across species (mouse, rabbit and human) and for different isoforms. NH3 recognises human filamentous actin (F-actin), but has been reported to recognise globular actin (G-actin).
• Isotype: IgM Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Thiophilic Chromatography

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Thiophilic Chromatography

Alternate Names for F-Actin Antibody (NH3) - BSA Free

• actin, cytoplasmic 2
• DFNA26
• ACT
• ACTG
• actin, gamma 1
• BRWS2
• DFNA20

Background

In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.