F4/80 Antibody (BM8) [PE]

Immunohistochemistry-Frozen: F4/80 Antibody (BM8) [PE] [NBP2-22134] - Red: F4/80, Green: CD31/PECAM-1, Blue: DAPI+ nuclei. Frozen mouse lung tissues of WT B6 and IL1R--/- (knockout) mice were blocked with 1% BSA in PBS and were double stained with PE-conjugated F4/80 and Alex647-conjugated CD31/PECAM-1 antibody (MEC 7.46) for 2 hours at the room temperature. This image was submitted via a customer review.

Flow Cytometry: F4/80 Antibody (BM8) [PE] [NBP2-22134] - Splenocytes isolated from a IL1R-/- mouse (IL1b receptor KO) were stained with F4/80 Antibody (BM8) and CD45-e450 (1:100 in FACS buffer) for 30 minutes. Cells were then washed and analyzed by flow cytometry. Left panel shows the isotype control. This image was submitted via a customer review.

Product Discontinued

F4/80 Antibody (BM8) [PE] Summary

• Immunogen: This F4/80 Antibody (BM8) was developed against BALB/c macrophages obtained from 14-day-old bone marrow cell cultures.
• Marker: Macrophage Marker
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: Adgre1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Knockout Validated
  • Western Blot 1:100-1:2000
• Application Notes: This antibody works in Flow Cytometry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot. The typical starting working dilution is 1:50. F4/80 is sensitive to 2-mercaptoethanol. The antibody requires proteinase K treatment of paraffin sections. IHC-P and Flow reported in a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS and 1.0% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for F4/80 Antibody (BM8) [PE]

• ADGRE1
• cell surface glycoprotein F4/80
• DD7A5-
• DD7A5-7
• EGF-like module containing, mucin-like, hormone receptor-like sequence 1
• EGF-like module receptor 1
• EGF-like module-containing mucin-like hormone receptor-like 1
• EGF-TM7
• Emr
• EMR1 hormone receptor
• Emr1
• F4/80
• Gpf480
• Ly7
• Ly71
• lymphocyte antigen 71
• Mouse EMR1
• Rat EMR1
• TM7L
• TM7LN3

Background

F4/80 antigen is a cell surface glycoprotein that is the murine homolog of human EGF-like module-containing mucin-like hormone receptor-like 1 (EMR1) (1,2). Specifically, F4/80 (EMR1) is one of five members belonging to the adhesion G-coupled receptor (ADGRE) subfamily, also known as the epidermal growth factor seven transmembrane (EGF-TM7) receptors (1,2). F4/80 is expressed by the myeloid lineage including in eosinophils, monocytes, macrophages, and myeloid dendritic cells (1,2). The molecular structure of F4/80 contains an EGF-like extracellular domain and a seven transmembrane motif, with a theoretical molecular weight of 160 kDa (1).

Depending on factors such as differentiation, activation, and location, the level of F4/80 expression can vary between cell-types (1,2). For example, peritoneal macrophages are subdivided based on morphology and expression with large peritoneal macrophages having high levels of F4/80 and small peritoneal macrophages having low F4/80 expression (1). Furthermore, splenic macrophages are classified based on location (red pulp, marginal zone, and white pulp) and F4/80 expression level varies within these populations as well; the red pulp macrophages have high levels of F4/80 and CD68 by comparison (1). Originally, the F4/80 monoclonal antibody was generated for a macrophage-specific surface antigen, however F4/80 staining is also observed in other hematopoietic cell populations, thus other immune cell markers should be used in conjunction with F4/80 to distinguish between different populations (1). Though the full extent of F4/80 ligands and biological functions remains elusive, studies have suggested that F4/80 is critical for macrophage-natural killer cell interaction and cytokine release (1,2). Additionally, a study of anterior chamber-associated immune deviation (ACAID) indicated a possible role for F4/80 in the generation of regulatory T cells (1,2).

References

1. Dos Anjos Cassado A. (2017). F4/80 as a Major Macrophage Marker: The Case of the Peritoneum and Spleen. Results and Problems in Cell Differentiation. https://doi.org/10.1007/978-3-319-54090-0_7

2. Hamann, J., Hsiao, C. C., Lee, C. S., Ravichandran, K. S., & Lin, H. H. (2016). Adhesion GPCRs as Modulators of Immune Cell Function. Handbook of Experimental Pharmacology. https://doi.org/10.1007/978-3-319-41523-9_15

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Reviews for F4/80 Antibody (NBP2-22134) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Mouse.

reviewed by: James Xiao IHC-Fr Mouse 03/02/2018

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Lung tissue
• Species: Mouse
• Lot: 14910M1113-C

Comments

• Comments: Frozen mouse lung tissues of WT B6 and IL1R--/- (knockout) mice were blocked with 1% BSA in PBS and were double stained with PE-conjugated F4/80 and Alex647-conjugated CD31/PECAM-1 antibody (MEC 7.46) for 2 hours at the room temperature.

reviewed by: James Xiao Flow Mouse 03/02/2018

Summary

• Application: Flow Cytometry
• Sample Tested: Mouse splenocytes
• Species: Mouse
• Lot: 14910M1113-C

Comments

• Comments: Splenocytes isolated from a IL1R-/- mouse (IL1b receptor KO) were stained with F4/80 Antibody (BM8) and CD45-e450 (1:100 in FACS buffer) for 30 minutes. Cells were then washed and analyzed by flow cytometry.

FAQs for F4/80 Antibody (NBP2-22134). (Showing 1 - 3 of 3 FAQ).

1. Are the F4/80/EMR1 antibodies validated in Simple Western?
   • No, F4/80/EMR1 antibodies are not tested in Simple Western at this moment.
2. Does F4/80/EMR1 antibodies come in lyophilized form?
   • Yes, we carry 2 F4/80 antibodies in lyophilized form: MAB5580 and NBP1-06975
3. What is the theoretical molecular weight for F4/80/EMR1 antibodies?
   • The TMW of F4/80/EMR1 antibodies is approximately 160 kDa.

Bioinformatics

• Gene Symbol: Adgre1