F4/80 Antibody (Cl:A3-1) - BSA Free

Immunohistochemistry: F4/80 Antibody (CI-A3-1) [NB600-404] - Oral treatment with L plantarum leads to recruitment of myeloid cells in kidney.Immunostaining of kidney sections from groups of treated mice, in the presence or absence of Leptospira infection using various leukocyte markers. We determined the number of F4/80+ T cells per millimeter squared of kidney. Data are mean SEM of 4-5 mice per group. Scale bar represents 400x. Statistics by two-tailed paired t-test *p < 0.05, *** p < 0.001. Data is representative of one of two experiments. Image collected and cropped by CiteAb from the following publication (doi.org/10.1371/journal.pntd.0005870) licensed under a CC-BY license.

Immunohistochemistry: F4/80 Antibody (CI-A3-1) [NB600-404] - Oral treatment with L plantarum leads to recruitment of myeloid cells in kidney.Immunostaining of kidney sections from groups of treated mice, in the presence or absence of Leptospira infection using various leukocyte markers. We determined the number of F4/80+ T cells per millimeter squared of kidney. Data are mean SEM of 4-5 mice per group. Scale bar represents 400x. Statistics by two-tailed paired t-test *p < 0.05, *** p < 0.001. Data is representative of one of two experiments. Image collected and cropped by CiteAb from the following publication (doi.org/10.1371/journal.pntd.0005870) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: F4/80 Antibody (CI-A3-1) [NB600-404] - Lymph node stained for macrophages, red and B cells CD79b, green; nuclei are stained with DAPI, blue.

Flow Cytometry: F4/80 Antibody (CI-A3-1) [NB600-404] - A surface stain was performed on RAW 246.7 cells with F4/80 antibody (CI-A3-1) NB600-404AF488 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 10 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Immunohistochemistry-Paraffin: F4/80 Antibody (CI-A3-1) [NB600-404] - pNAPE-EcN reduces diet-induced hepatic inflammation. Representative images of liver sections immunostained for F4/80. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-37373-1), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: F4/80 Antibody (CI-A3-1) [NB600-404] - Analysis of monocytes cultured in M-CSF using F4/80 antibody. Primary antibody dilution: 1:200. Overnight incubation in RT. Image from verified customer review.

Flow Cytometry: F4/80 Antibody (CI-A3-1) - BSA Free [NB600-404] - Surface staining of F4/80 in CT26 colorectal carcinoma tumor model. Using Allophycocyanin conjugated version of the antibody (NB600-404APC). Image from verified customer review

Immunohistochemistry-Frozen: F4/80 Antibody (CI-A3-1) [NB600-404] - Frozen mouse spleen with F4/80 anti-mouse antibody.

Immunohistochemistry-Frozen: F4/80 Antibody (CI-A3-1) [NB600-404] - Mouse spleen stained with A: Rat anti Mouse. B: CI-A3-1 clone preincubated with 2 molar excess of human anti Idiotypic.

Flow Cytometry: F4/80 Antibody (CI-A3-1) [NB600-404] - Staining of J774 cells with F4/80 antibody.

Flow Cytometry: F4/80 Antibody (CI-A3-1) [NB600-404] - Staining of mouse peritoneal macrophages with clone CI-A3-1 F4/80 antibody.

Flow Cytometry: F4/80 Antibody (CI-A3-1) [NB600-404]

Immunohistochemistry: F4/80 Antibody (Cl:A3-1) [NB600-404] - Lymphocyte interaction inside the DC harboring scaffoldsDC scaffolds (beDC) or scaffolds only, placed in tumor bearing mice or DC-scaffold placed in normal mice were recovered two weeks post-implantation & processed for immunohistochemistry to demonstrate various cell types. (A) Relative size of the biomatrices harvested from various mice groups as depicted in the figure (far left panel). n = 3–5. The dotted line in ‘gross specimen’ differentiates the biomatrix from the reactive host-tissue. Representative images of biomatrix sections stained by the respective antibodies as indicated in the figure. The bar in the ‘gross structure’ is equivalent to 1 mm, whereas for other panels is equal to 100 μm. (B) Snapshots from Supplementary Movie S2 showing lymphocyte (green) movement & interaction with DCs (red) inside the biomatrix. The start point of the observation was set as the zero time point. (C) Lymphocyte division (white arrows) inside the biomatrix in the vicinity of DC (red). Representative snapshot from Supplementary Movie S3. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27223090), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Mu
• Applications: WB, Flow, Func, ICC/IF, IHC, IP
• Clone: Cl:A3-1
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

F4/80 Antibody (Cl:A3-1) - BSA Free Summary

• Immunogen: Thioglycollate stimulated peritoneal macrophages from C57BL/6 mice
• Localization: Cell surface
• Marker: Macrophage Marker
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: Adgre1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500RadioImmunoassay 1:100-1:2000
  • Radioimmunoassay 1:100-1:2000
  • Western Blot 1:100-1:2000
• Application Notes: This product requires pretreatment of paraffin sections prior to staining. Proteinase K is recommended for tissues fixed for less than 24 hours. Citrate buffer pH 6.0 is recommended for tissues fixed for more than 24 hours. E. coli reactivity reported in scientific literature (PMID:31181736).
• Theoretical MW: 160 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for F4/80 Antibody (Cl:A3-1) - BSA Free

• ADGRE1
• cell surface glycoprotein F4/80
• DD7A5-
• DD7A5-7
• EGF-like module containing, mucin-like, hormone receptor-like sequence 1
• EGF-like module receptor 1
• EGF-like module-containing mucin-like hormone receptor-like 1
• EGF-TM7
• Emr
• EMR1 hormone receptor
• Emr1
• F4/80
• Gpf480
• Ly7
• Ly71
• lymphocyte antigen 71
• Mouse EMR1
• Rat EMR1
• TM7L
• TM7LN3

Background

F4/80 antigen is a cell surface glycoprotein that is the murine homolog of human EGF-like module-containing mucin-like hormone receptor-like 1 (EMR1) (1,2). Specifically, F4/80 (EMR1) is one of five members belonging to the adhesion G-coupled receptor (ADGRE) subfamily, also known as the epidermal growth factor seven transmembrane (EGF-TM7) receptors (1,2). F4/80 is expressed by the myeloid lineage including in eosinophils, monocytes, macrophages, and myeloid dendritic cells (1,2). The molecular structure of F4/80 contains an EGF-like extracellular domain and a seven transmembrane motif, with a theoretical molecular weight of 160 kDa (1).

Depending on factors such as differentiation, activation, and location, the level of F4/80 expression can vary between cell-types (1,2). For example, peritoneal macrophages are subdivided based on morphology and expression with large peritoneal macrophages having high levels of F4/80 and small peritoneal macrophages having low F4/80 expression (1). Furthermore, splenic macrophages are classified based on location (red pulp, marginal zone, and white pulp) and F4/80 expression level varies within these populations as well; the red pulp macrophages have high levels of F4/80 and CD68 by comparison (1). Originally, the F4/80 monoclonal antibody was generated for a macrophage-specific surface antigen, however F4/80 staining is also observed in other hematopoietic cell populations, thus other immune cell markers should be used in conjunction with F4/80 to distinguish between different populations (1). Though the full extent of F4/80 ligands and biological functions remains elusive, studies have suggested that F4/80 is critical for macrophage-natural killer cell interaction and cytokine release (1,2). Additionally, a study of anterior chamber-associated immune deviation (ACAID) indicated a possible role for F4/80 in the generation of regulatory T cells (1,2).

References

1. Dos Anjos Cassado A. (2017). F4/80 as a Major Macrophage Marker: The Case of the Peritoneum and Spleen. Results and Problems in Cell Differentiation. https://doi.org/10.1007/978-3-319-54090-0_7

2. Hamann, J., Hsiao, C. C., Lee, C. S., Ravichandran, K. S., & Lin, H. H. (2016). Adhesion GPCRs as Modulators of Immune Cell Function. Handbook of Experimental Pharmacology. https://doi.org/10.1007/978-3-319-41523-9_15

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for F4/80 Antibody (NB600-404)(103)

Publications using NB600-404

• Alexander KA, Tseng HW, Lao HW, Girard D et Al. A glucocorticoid spike derails muscle repair to heterotopic ossification after spinal cord injury Cell Rep Med 2024-12-10 [PMID: 39657663]
• Good CJ, Butrico CE, Colley ME, Emmerson LN et Al. Uncovering lipid dynamics in Staphylococcus aureus osteomyelitis using multimodal imaging mass spectrometry Cell Chem Biol 2024-10-10 [PMID: 39389064]
• Chen L, Qin Y, Guo T et Al. HAP40 modulates mutant Huntingtin aggregation and toxicity in Huntington's disease mice Cell Death Dis 2024-05-14 [PMID: 38744826]
• Thangavel H, Dhanyalayam D, Kim M, Lizardo K et Al. Adipocyte-released adipomes in Chagas cardiomyopathy: Impact on cardiac metabolic and immune regulation iScience 2024-04-25 [PMID: 38660407]
• Giuseppe Angelini, Emanuele Capra, Francesca Rossi, Giada Mura, Marielle Saclier, Valentina Taglietti, Gabriele Rovetta, Raffaele Epis, Giorgia Careccia, Chiara Bonfanti, Graziella Messina MEK-inhibitors decrease Nfix in muscular dystrophy but induce unexpected calcifications, partially rescued with Cyanidin diet iScience 2023-12-10 [PMID: 38205246]
• Dailey KM, Small JM, Pullan JE et al. An intravenous pancreatic cancer therapeutic: Characterization of CRISPR/Cas9n-modified Clostridium novyi-Non Toxic PloS one 2023-11-14 [PMID: 37963142] (IHC-P, Mouse)
• Zheng L, Ling W, Zhu D et al. Roquin-1 resolves sepsis-associated acute liver injury by regulating inflammatory profiles via miRNA cargo in extracellular vesicles iScience 2023-08-01 [PMID: 37554446] (ICC/IF, Mouse)
• Imamura Y, Matsumoto H, Imamura J et al. Ultrasound stimulation of the vagal nerve improves acute septic encephalopathy in mice Frontiers in neuroscience 2023-07-17 [PMID: 37529234] (Mouse)
• Acosta J, Li Q, Freeburg NF et al. p53 restoration in small cell lung cancer identifies a latent cyclophilin-dependent necrosis mechanism Nature communications 2023-07-21 [PMID: 37479684] (IHC-P)
• Huang D, Kidd JM, Zou Y et al. Regulation of NLRP3 Inflammasome Activation and Inflammatory Exosome Release in Podocytes by Acid Sphingomyelinase During Obesity Inflammation 2023-07-21 [PMID: 37477734]
• Chiang CH, Chen C, Fang SY et al. Xanthine oxidase/NADPH oxidase inhibition by hydralazine attenuates acute kidney injury and prevents the transition of acute kidney injury to chronic kidney disease Life sciences 2023-08-15 [PMID: 37331504] (IHC-P, Mouse)
• Owen AM, Luan L, Burelbach KR et al. MyD88-dependent signaling drives toll-like receptor-induced trained immunity in macrophages Frontiers in immunology 2022-11-11 [PMID: 36439136] (IF/IHC, Mouse)
• Stewart TA, Hughes K, Hume DA, Davis FM Developmental stage-specific distribution of macrophages in mouse mammary gland bioRxiv (IHC-P, ICC/IF, Mouse)
• Chiang CH, Lan TY, Hsieh JH et Al. Diosgenin Reduces Acute Kidney Injury and Ameliorates the Progression to Chronic Kidney Disease by Modifying the NOX4/p65 Signaling Pathways J Agric Food Chem 2024-07-29 [PMID: 39074384]
• Xiao T, Liang J, Li M et Al. ATG5-mediated keratinocyte ferroptosis promotes M1 polarization of macrophages to aggravate UVB-induced skin inflammation J Photochem Photobiol B 2024-07-14 [PMID: 38833786]
• Miyamoto Y, Hasegawa T, Hongo H et al. Histochemical assessment of osteoclast-like giant cells in Rankl-/- mice Journal of oral biosciences 2023-04-21 [PMID: 37088151]
• Chang TT, Li SY, Lin LY et al. Macrophage inflammatory protein-1? as a novel therapeutic target for renal protection in diabetic kidney disease Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2023-02-28 [PMID: 36863097] (IHC-Fr, Mouse)
• Tanaka T, Okuda H, Isonishi A et al. Dermal macrophages set pain sensitivity by modulating the amount of tissue NGF through an SNX25-Nrf2 pathway Nature immunology 2023-01-26 [PMID: 36703006] (IHC-Fr, Mouse)
  
  Details:
  Dilution used 1:500
• Oswal N, Thangavel H, Lizardo K et al. Diets Differently Regulate Pulmonary Pathogenesis and Immune Signaling in Mice during Acute and Chronic Mycobacterium tuberculosis Infection Life (Basel, Switzerland) 2023-01-13 [PMID: 36676177] (WB, Mouse)
• Liu X, Zhang H, Yan B et al. On-Off Phagocytosis and Switchable Macrophage Activation Stimulated with NIR for Infected Percutaneous Tissue Repair of Polypyrrole-Coated Sulfonated PEEK Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2022-12-14 [PMID: 36515274] (IHC-P, Mouse)
  
  Details:
  Dilution used in IHC-P 1:50
• Janani G, Zhang L, Badylak S et al. Silk fibroin bioscaffold from Bombyx mori and Antheraea assamensis elicits a distinct host response and macrophage activation paradigm in vivo and in vitro Biomaterials Advances 2022-11-01 [PMID: 36502549] (ICC/IF, Mouse)
• Balko J, Axelrod M, Meijers W et al. Cytotoxic T cells specific for alpha-myosin drive immunotherapy related myocarditis Research Square 2022-02-04 [PMID: 36385524] (IHC-P, Mouse)
• Axelrod ML, Meijers WC, Screever EM et al. T cells specific for alpha -myosin drive immunotherapy-related myocarditis Nature 2022-11-01 [PMID: 36385524] (IHC-P, Mouse)
• Schleef M, Gonnot F, Pillot B et al. Mild Therapeutic Hypothermia Protects from Acute and Chronic Renal Ischemia-Reperfusion Injury in Mice by Mitigated Mitochondrial Dysfunction and Modulation of Local and Systemic Inflammation International journal of molecular sciences 2022-08-17 [PMID: 36012493] (IHC-Fr, Mouse)
  
  Details:
  Eight- to ten-week-old male C57BL6 mice, renal tissue dilution used 1:100
• Zhang T, Yin C, Fedorov A et al. ADAR1 masks the cancer immunotherapeutic promise of ZBP1-driven necroptosis Nature 2022-05-25 [PMID: 35614224] (ICC/IF, Mouse)
  
  Details:
  See Extended Data Fig. 5
• Salman S, Meyers DJ, Wicks EE Et al. HIF inhibitor 32-134D eradicates murine hepatocellular carcinoma in combination with anti-PD1 therapy J Clin Invest 2022-05-02 [PMID: 35499076] (FLOW, Mouse)
  
  Details:
  Citation using the Alexa Fluor 488 version of this antibody.
• Gerasimenko J, Petersen O, Gerasimenko O SARS-CoV-2 S Protein Subunit 1 Elicits Ca2+ Influx - Dependent Ca2+ Signals in Pancreatic Stellate Cells and Macrophages In Situ Function 2022-02-15 [PMID: 35284826] (ICC/IF, Mouse)
• Lin X, Song F, Xue D, Wang Y Lycium Barbarum Polysaccharide Attenuates Pseudomonas-Aeruginosa Pyocyanin Induced Cellular Injury in Mice Airway Epithelial Cells Food Nutr Res 2022-03-09 [PMID: 35261577]
• Keshvari S, Genz B, Teakle N et al. Therapeutic potential of macrophage colony-stimulating factor in chronic liver disease Disease models & mechanisms 2022-04-01 [PMID: 35169835] (IF/IHC, Mouse)
• Ribeiro A, Dobosz E, Krill M et al. Macrophage-Specific MCPIP1/Regnase-1 Attenuates Kidney Ischemia-Reperfusion Injury by Shaping the Local Inflammatory Response and Tissue Regeneration Cells 2022-01-24 [PMID: 35159206]
• Manigat LC, Granade ME, Taori S Et al. Loss of Diacylglycerol Kinase alpha Enhances Macrophage Responsiveness Frontiers in immunology 2021-11-05 [PMID: 34804012] (IF/IHC, Mouse)
• Sang Y, Tsuji K, Fukushima K Et al. Semaporin3A inhibitor ameliorates renal fibrosis through the regulation of JNK signaling American journal of physiology. Renal physiology 2021-12-01 [PMID: 34747196]
• Su W, Sun S, Tian B et al. Efficacious, safe and stable inhibition of corneal neovascularization by AAV vectored anti-VEGF therapeutics Mol Ther Methods Clin Dev 2021-09-13 [PMID: 34514023]
• Reinfeld BI, Madden MZ, Wolf MM et al. Cell-programmed nutrient partitioning in the tumour microenvironment Nature 2021-05-13 [PMID: 33828302]
• Chen L, Wu H, Ren C, et al. Inhibition of PDGF-BB reduces alkali-induced corneal neovascularization in mice Molecular Medicine Reports 2021-01-28 [PMID: 33537811]
• Chiba T, Cerqueira DM, Li Y, et al. Endothelial-Derived miR-17∼92 Promotes Angiogenesis to Protect against Renal Ischemia-Reperfusion Injury Journal of the American Society of Nephrology : JASN 2021-01-29 [PMID: 33514560]
• Kim S, Kim SA, Nam GH, et al. In situ immunogenic clearance induced by a combination of photodynamic therapy and rho-kinase inhibition sensitizes immune checkpoint blockade response to elicit systemic antitumor immunity against intraocular melanoma and its metastasis Journal for immunotherapy of cancer 2021-01-01 [PMID: 33479026] (IHC-P, Mouse)
• Dai Q, Zhang Y, Liao X et al. Fluorofenidone Alleviates Renal Fibrosis by Inhibiting Necroptosis Through RIPK3/MLKL Pathway Frontiers in pharmacology 2020-12-16 [PMID: 33390935] (IHC-P, Mouse)
• Nishiwaki S, Saito S, Takeshita K et al. In vivo tracking of transplanted macrophages with near infrared fluorescent dye reveals temporal distribution and specific homing in the liver that can be perturbed by clodronate liposomes PloS one 2020-12-10 [PMID: 33301448] (IHC-P, Mouse)
• Wei, S C, Meijers, W C Et al. A Genetic Mouse Model Recapitulates Immune Checkpoint Inhibitor-Associated Myocarditis and Supports a Mechanism-Based Therapeutic Intervention. Cancer Discov 2021-03-01 [PMID: 33257470] (IHC, Mouse)
• Wang L, Sinnott-Armstrong N, Wagschal A et al. A MicroRNA Linking Human Positive Selection and Metabolic Disorders Cell 2020-10-29 [PMID: 33058756] (Mouse)
• Li Z, Bian X, Ma Y et al. Uterine scar leads to adverse pregnant consequence through impairing the response of endometrium to steroids Endocrinology 2020-09-25 [PMID: 32976565] (Human)
• Rai V, Wood MB, Feng H et al. The immune response after noise damage in the cochlea is characterized by a heterogeneous mix of adaptive and innate immune cells Scientific reports 2020-09-16 [PMID: 32938973] (IHC-Fr, Mouse)
• Chang TT, Yang HY, Chen C, Chen JW. CCL4 Inhibition in Atherosclerosis: Effects on Plaque Stability, Endothelial Cell Adhesiveness, and Macrophages Activation International Journal of Molecular Sciences 2020-09-08 [PMID: 32911750]
• Samanta D, Huang T, Shah R et al. BIRC2 Expression Impairs Anti-Cancer Immunity and Immunotherapy Efficacy Cell Rep [PMID: 32846130] (FLOW, Mouse)
  
  Details:
  Citation using the PE format of this antibody.
• Hozain S, Cottrell J CDllb+ targeted depletion of macrophages negatively affects bone fracture healing Bone 2020-06-12 [PMID: 32535290] (IHC-P, Mouse)
• Mokshagundam S, Ding T, Rumph JT et al. Developmental 2,3,7,8-tetrachlorodibenzo-p-dioxin exposure of either parent enhances the risk of necrotizing enterocolitis in neonatal mice Birth Defects Res 2020-06-09 [PMID: 32519502]
• Ayturk UM, Scollan JP, Goz Ayturk D et al. Single-Cell RNA Sequencing of Calvarial and Long-Bone Endocortical Cells Journal of Bone and Mineral Research 2020-10-01 [PMID: 32427356]
• Vasiukov G, Novitskaya T, Zijlstra A et al. Myeloid cell-derived TGF-beta signaling regulates ECM deposition in mammary carcinoma via adenosine-dependent mechanisms Cancer Res. 2020-04-20 [PMID: 32312837] (IHC-P, Mouse)
• Elkabes S Astroglial TLR9 antagonism promotes chemotaxis and alternative activation of macrophages via modulation of astrocyte-derived signals: implications for spinal cord injury J Neuroinflammation 2020-02-25 [PMID: 32098620] (WB, Mouse)
• Hasenour CM, Kennedy AJ, Bednarski T, Trenary IA et al. Vitamin E does not prevent Western diet induced NASH progression and increases metabolic flux dysregulation in mice Journal of Lipid Research 2020-02-21 [PMID: 32086244] (IF/IHC, Mouse)
• Zou D, Pei J, Lan J et al. A SNP of bacterial blc disturbs gut lysophospholipid homeostasis and induces inflammation through epithelial barrier disruption EBioMedicine 2020-02-12 [PMID: 32058942] (IHC-P, C. elegans)
• Novitskiy SV, Zaynagetdinov R, Vasiukov G et al. Gas6/MerTK signaling is negatively regulated by NF-kappa B and supports lung carcinogenesis Oncotarget 2019-12-17 [PMID: 31903163] (IF/IHC, Mouse)
• Matoba H, Takamoto M, Fujii C et al. Cecal tumorigenesis in AhR-deficient mice depends on cecum-specific MAPK pathway activation and inflammation Am. J. Pathol. 2019-11-14 [PMID: 31734232]
• Stewart T A, Hughes K et al. Developmental Stage-Specific Distribution of Macrophages in Mouse Mammary Gland. Front Cell Dev Biol 2019-12-11 [PMID: 31709255] (IF/IHC, Mouse)
• Terasaki Y, Terasaki M, Kanazawa S et al. Effect of H2 treatment in a mouse model of rheumatoid arthritis-associated interstitial lung disease J. Cell. Mol. Med. 2019-08-19 [PMID: 31424157] (IF/IHC, Mouse)
• Tan W, Tang H, Jiang X et al. Metformin mediates induction of miR-708 to inhibit self-renewal and chemoresistance of breast cancer stem cells through targeting CD47 J. Cell. Mol. Med. 2019-07-05 [PMID: 31273952]
  
  Details:
  Phagocytosis Assay
• Tawfik A, Knight P, Duckworth CA et al. Replication of Crohn's Disease Mucosal E. coli Isolates inside Macrophages Correlates with Resistance to Superoxide and Is Dependent on Macrophage NF-kappa B Activation Pathogens 2019-06-08 [PMID: 31181736] (ICC, E. coli)
• Oo ZY, Proctor M, Stevenson AJ, Nazareth D et al. Combined use of subclinical hydroxyurea and CHK1 inhibitor effectively controls melanoma and lung cancer progression, with reduced normal tissue toxicity compared to gemcitabine Mol Oncol 2019-05-01 [PMID: 31044505] (IHC-P, Mouse)
• He, Y;Li, S;Tang, D;Peng, Y;Meng, J;Peng, S;Deng, Z;Qiu, S;Liao, X;Chen, H;Tu, S;Tao, L;Peng, Z;Yang, H; Circulating Peroxiredoxin-1 is a novel damage-associated molecular pattern and aggravates acute liver injury via promoting inflammation Free Radic. Biol. Med. 2019-04-13 [PMID: 30991142] (IF/IHC, Mouse)
• Lei, J;Vermillion, MS;Jia, B;Xie, H;Xie, L;McLane, MW;Sheffield, JS;Pekosz, A;Brown, A;Klein, SL;Burd, I; IL-1 receptor antagonist therapy mitigates placental dysfunction and perinatal injury following Zika virus infection JCI Insight 2019-04-04 [PMID: 30944243] (IF/IHC, Mouse)
• Bhatia S, Oweida A, Lennon S et al. Inhibition of EphB4-ephrin-B2 signaling reprograms the tumor immune microenvironment in head and neck cancers Cancer Res. 2019-03-20 [PMID: 30894369] (ICC/IF, Human)
• Terasaki Y, Suzuki T, Tonaki K et al. Molecular hydrogen attenuates gefitinib-induced exacerbation of naphthalene-evoked acute lung injury through a reduction in oxidative stress and inflammation Lab. Invest. 2019-02-01 [PMID: 30710119] (IF/IHC, Mouse)
• May-Zhang LS, Chen Z, Dosoky NS et al. Administration of N-Acyl-Phosphatidylethanolamine Expressing Bacteria to Low Density Lipoprotein Receptor-/- Mice Improves Indices of Cardiometabolic Disease. Sci Rep 2019-01-23 [PMID: 30674978] (IHC-Fr, Mouse)
• Laroumanie F, korneva A, Bersi MR et al. LNk deficiency promotes acute aortic dissection and rupture. JCI Insight. 2018-10-18 [PMID: 30333305] (IHC-P, Mouse)
• Zhang Y, Kirane A, Huang H et al. Cyclooxygenase-2 inhibition potentiates the efficacy of vascular endothelial growth factor blockade and promotes an immune stimulatory microenvironment in preclinical models of pancreatic cancer. Mol. Cancer Res. 2018-10-17 [PMID: 30333153] (IHC-P, Mouse)
• Kim SE, Park JW, Kim MJ et al. Accumulation of citrullinated glial fibrillary acidic protein in a mouse model of bile duct ligation-induced hepatic fibrosis PLoS ONE 2018-08-02 [PMID: 30071078] (IHC-P, Mouse)
• Su Li, Li Na, Tang Hua et al. Kupffer cell-derived TNF-alpha promotes hepatocytes to produce CXCL1 and mobilize neutrophils in response to necrotic cells. Cell Death & Disease 2018-02-23 [PMID: 29476069] (IHC-P, Mouse)
• Iacobini C, Fantauzzi CB, Bedini R, Pecci R. Galectin-3 is essential for proper bone cell differentiation and activity, bone remodeling and biomechanical competence in mice. Metab. Clin. Exp. 2018-02-09 [PMID: 29432728] (IF/IHC, Mouse)
• Lietman C, Wu B, Lechner S, Shinar A. Inhibition of Wnt/b-catenin signaling ameliorates osteoarthritis in a murine model of experimental osteoarthritis. JCI Insight. 2018-02-08 [PMID: 29415892] (IHC-P, Mouse)
• Xiao T, Liang J, Li M et al. ATG5-Mediated Keratinocyte Ferroptosis Promotes M1 Polarization&nbsp;Of Macrophages to Aggravate UVB-Induced Skin Inflammation SSRN 2023-10-04 (IHC, Mouse)
• Fensterheim BA, Young JD, Luan L et al. The TLR4 Agonist Monophosphoryl Lipid A Drives Broad Resistance to Infection via Dynamic Reprogramming of Macrophage Metabolism. J Immunol 2018-06-01 [PMID: 29686054]
• Batoon L, Millard SM, Wullschleger ME et al. CD169+ macrophages are critical for osteoblast maintenance and promote intramembranous and endochondral ossification during bone repair. Biomaterials. [PMID: 29107337] (IHC-P, Mouse)
• Moon H, Donahue LR, Choi E et al. Melanocyte Stem Cell Activation and Translocation Initiate Cutaneous Melanoma in Response to UV Exposure. Cell Stem Cell. 2017-10-04 [PMID: 29033353] (WB)
• Potula HH, Richer L, Werts C, Gomes-Solecki M. Pre-treatment with Lactobacillus plantarum prevents severe pathogenesis in mice infected with Leptospira interrogans and may be associated with recruitment of myeloid cells PLoS Negl Trop Dis 2017-08-25 [PMID: 28841659] (IHC-P, Mouse)
• Yuan H, Jiang W, von Roemeling CA et al. Multivalent bi-specific nanobioconjugate engager for targeted cancer immunotherapy. Nat Nanotechnol 2017-05-01 [PMID: 28459470] (IHC-P)
• Chung KJ, Chatzigeorgiou A, Economopoulou M et al. A self-sustained loop of inflammation-driven inhibition of beige adipogenesis in obesity. Nat. Immunol. 2017-04-17 [PMID: 28414311]
• Wang W, Xu MJ, Cai Y et al. Inflammation is independent of steatosis in a murine model of steatohepatitis.. Hepatology. 2017-02-21 [PMID: 28220523] (IHC-P, Mouse)
• Verma V, Kim Y et al. Activated dendritic cells delivered in tissue compatible biomatrices induce in-situ anti-tumor CTL responses leading to tumor regression. Oncotarget 2016-06-28 [PMID: 27223090] (IF/IHC, Mouse)
• Demonbreun AR, Allen MV, Warner JL et al. Enhanced Muscular Dystrophy from Loss of Dysferlin Is Accompanied by Impaired Annexin A6 Translocation after Sarcolemmal Disruption. Am. J. Pathol. 2016-04-09 [PMID: 27070822] (ICC/IF, IHC-Fr, Mouse)
  
  Details:
  This publication used the AF488 conjugated form of this antibody (NB600-404AF488)
• Zhang H, Lu H, Xiang L et al. HIF-1 regulates CD47 expression in breast cancer cells to promote evasion of phagocytosis and maintenance of cancer stem cells. Proc Natl Acad Sci. 2015-11-10 [PMID: 26512116] (FLOW, Human)
  
  Details:
  This citation used the Allophycocyanin form of this antibody.
• Ge X, Pettan-Brewer C, Morton J et al. Mitochondrial catalase suppresses naturally occurring lung cancer in old mice. Pathobiol Aging Age Relat Dis 2015-09-24 [PMID: 26400209] (IF/IHC, Mouse)
• Merches K, Khairnar V, Knuschke T et al. Virus-Induced Type I Interferon Deteriorates Control of Systemic Pseudomonas Aeruginosa Infection Cell Physiol Biochem 2015-08-17 [PMID: 26279441] (IHC-Fr, Mouse)
  
  Details:
  This citation used the DyLight 488 version of this antibody.
• Saleh MA, McMaster WG, Wu J et al. Lymphocyte adaptor protein LNK deficiency exacerbates hypertension and end-organ inflammation J. Clin. Invest. 2015-03-02 [PMID: 25664851] (IHC-P, Mouse)
• Nishiwaki S, Nakayama T, Murata M et al. Dexamethasone Palmitate Ameliorates Macrophages-Rich Graft-versus-Host Disease by Inhibiting Macrophage Functions. PLoS ONE. 2014-05-08 [PMID: 24806147] (IHC-P, Mouse)
• Kwon HJ, Won YS, Park O et al. Aldehyde dehydrogenase 2 deficiency ameliorates alcoholic fatty liver but worsens liver inflammation and fibrosis in mice. Hepatology 2014-02-04 [PMID: 24492981] (IHC-P, Mouse)
• Oh JY, Lee HJ, Ko AY et al. Analysis of macrophage phenotype in rejected corneal allografts. Invest Ophthalmol Vis Sci. 2013-10-24 [PMID: 24159090]
• Phieler J, Chung KJ, Chatzigeorgiou A et al. The complement anaphylatoxin c5a receptor contributes to obese adipose tissue inflammation and insulin resistance. J Immunol. 2013-10-15 [PMID: 24043887] (IHC-P, Mouse)
• Crowder S W, Horton L W et al. Passage-dependent cancerous transformation of human mesenchymal stem cells under carcinogenic hypoxia. FASEB J 2013-01-07 [PMID: 23568779] (IHC-P, Human)
• Harusato, A, Naito, Y Et al. BTB and CNC homolog 1 (Bach1) deficiency ameliorates TNBS colitis in mice: role of M2 macrophages and heme oxygenase-1. Inflamm Bowel Dis 2013-03-01 [PMID: 23446334] (WB, Mouse)
• Xiao C, Wang RH, Lahusen TJ et al. Progression of Chronic Liver Inflammation and Fibrosis Driven by Activation of c-JUN Signaling in Sirt6 Mutant Mice J Biol Chem 2012-10-16 [PMID: 23076146] (IF/IHC, Mouse)
• Karasawa F, Shiota A, Goso Y et al. Essential role of gastric gland mucin in preventing gastric cancer in mice J Clin Invest 2012-03-01 [PMID: 22307328] (IF/IHC, Mouse)
• Ouvrier A, Alves G, Damon-SoubeyrC et al. Dietary cholesterol-induced post-testicular infertility. PLoS ONE 20116(11). [PMID: 22073227]
• Gonzalez JM, Dong Z, Romero R, Girardi G. Cervical remodeling/ripening at term and preterm delivery: the same mechanism initiated by different mediators and different effector cells. PLoS ONE 20116(11):e26877. [PMID: 22073213]
• Harusato A, Naito Y, Takagi T et al. Suppression of indomethacin-induced apoptosis in the small intestine due to Bach1 deficiency. Free Radic Res 2011-04-08 [PMID: 21473739] (ICC/IF, IF/IHC, Mouse)
• Ramirez NE, Zhang Z, Madamanchi A et al. The alpha2beta1 integrin is a metastasis suppressor in mouse models and human cancer. J Clin Invest;121(1):226-37. 2011-01-01 [PMID: 21135504]
• Abrahamson EE, Ikonomovic MD, Dixon CE, DeKosky ST. Simvastatin therapy prevents brain trauma-induced increases in beta-amyloid peptide levels. Ann Neurol 2009-09-01 [PMID: 19798641] (IHC-Fr, Mouse)
• Jin Yiping, Arita Makoto, Zhang Qiang et al. Anti-angiogenesis effect of the novel anti-inflammatory and pro-resolving lipid mediators. Investigative Ophthalmology & Visual Science 2009-01-01 [PMID: 19407006] (IF/IHC, Mouse)
• Jaruga, B et al. Chronic alcohol consumption accelerates liver injury in T cell-mediated hepatitis: alcohol disregulation of NF-kB STAT3 signaling pathways. Am. J. Physiol: Gastrointestinal Liver Physiol. 287: 471-479. 2004-01-01 [PMID: 15064234]
• Austyn, JM Gordon, S. F4/80 , a monoclonal antibody directed specifically against the mouse macrophage. 1981-01-01 [PMID: 7308288]
• Hume, DA, Perry, VH, Gordon, S. The Mononuclear Phagocyte System of the Mouse defined by Immunohistochemical Localisation of Antigen F4/80: Macrophages Associated with Epithelia. 1984-01-01 [PMID: 6524692]
• Lee, SH et al. Quantitative analysis of total macrophage content in adult mouse tissues. Immunochemical studies with monoclonal antibody F4/80. 1985-01-01 [PMID: 3973536]
• Gordon, S et al. (1992) Antigen markers of macrophage differentiation in murine tissues. 1992-01-01 [PMID: 1424778]

Reviews for F4/80 Antibody (NB600-404) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Mouse.

reviewed by: Mozhdeh Sojoodi ICC Mouse 02/07/2022

Summary

• Application: Immunocytochemistry
• Sample Tested: Bone Marrrow Derived Macrophages
• Species: Mouse
• Lot: 1806

Comments

• Comments: 1:200 Dilution
  Overnight incubation in RT

reviewed by: Naiche Adler IHC-P Mouse 03/26/2015

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: mouse lymph node FFPE
• Species: Mouse

FAQs for F4/80 Antibody (NB600-404). (Showing 1 - 4 of 4 FAQs).

1. Are the F4/80/EMR1 antibodies validated in Simple Western?  ..show answer..
   • No, F4/80/EMR1 antibodies are not tested in Simple Western at this moment.
2. Does F4/80/EMR1 antibodies come in lyophilized form?  ..show answer..
   • Yes, we carry 2 F4/80 antibodies in lyophilized form: MAB5580 and NBP1-06975
3. What is the theoretical molecular weight for F4/80/EMR1 antibodies?  ..show answer..
   • The TMW of F4/80/EMR1 antibodies is approximately 160 kDa.
4. I'm hoping to use F4/80 as a macrophage marker in skeletal muscle using IHC-Fr but I haven't seen this done successfully (and heard people have tried without much luck). Do you know of any validation that has been done on that tissue?  ..show answer..
   • We have not validated NB600-404 on skeletal muscle specifically, and we haven't yet had any customer feedback regarding this antibody being used on skeletal muscle. If you are interested in using this antibody for your testing, and have any trouble getting it to work for you, please let us know. We do offer a guarantee on our products for the species and applications listed, and if we can't get this product working for you, we will compensate you for the purchase.

Bioinformatics

• Gene Symbol: Adgre1
• Entrez:
  • 13733(Mouse)
• Uniprot:
  • Q61549()
  • Q61549()