F4/80 Antibody (Cl:A3-1) [Alexa Fluor® 488]

Flow Cytometry: F4/80 Antibody (CI-A3-1) [Alexa Fluor (R) 488] [NB600-404AF488] - A surface stain was performed on RAW 246.7 cells with F4/80 antibody (CI-A3-1) NB600-404AF488 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 10 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

• Reactivity: Mu
• Applications: WB, Flow, Func, ICC/IF, IHC, IP, MS
• Clone: Cl:A3-1
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 488

F4/80 Antibody (Cl:A3-1) [Alexa Fluor® 488] Summary

• Description: This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
• Immunogen: Thioglycollate stimulated peritoneal macrophages from C57BL/6 mice
• Localization: Cell surface
• Marker: Macrophage Marker
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: Adgre1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Electron Microscopy
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Radioimmunoassay
  • Western Blot
• Theoretical MW: 160 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for F4/80 Antibody (Cl:A3-1) [Alexa Fluor® 488]

• ADGRE1
• cell surface glycoprotein F4/80
• DD7A5-
• DD7A5-7
• EGF-like module containing, mucin-like, hormone receptor-like sequence 1
• EGF-like module receptor 1
• EGF-like module-containing mucin-like hormone receptor-like 1
• EGF-TM7
• Emr
• EMR1 hormone receptor
• Emr1
• F4/80
• Gpf480
• Ly7
• Ly71
• lymphocyte antigen 71
• Mouse EMR1
• Rat EMR1
• TM7L
• TM7LN3

Background

F4/80 antigen is a cell surface glycoprotein that is the murine homolog of human EGF-like module-containing mucin-like hormone receptor-like 1 (EMR1) (1,2). Specifically, F4/80 (EMR1) is one of five members belonging to the adhesion G-coupled receptor (ADGRE) subfamily, also known as the epidermal growth factor seven transmembrane (EGF-TM7) receptors (1,2). F4/80 is expressed by the myeloid lineage including in eosinophils, monocytes, macrophages, and myeloid dendritic cells (1,2). The molecular structure of F4/80 contains an EGF-like extracellular domain and a seven transmembrane motif, with a theoretical molecular weight of 160 kDa (1).

Depending on factors such as differentiation, activation, and location, the level of F4/80 expression can vary between cell-types (1,2). For example, peritoneal macrophages are subdivided based on morphology and expression with large peritoneal macrophages having high levels of F4/80 and small peritoneal macrophages having low F4/80 expression (1). Furthermore, splenic macrophages are classified based on location (red pulp, marginal zone, and white pulp) and F4/80 expression level varies within these populations as well; the red pulp macrophages have high levels of F4/80 and CD68 by comparison (1). Originally, the F4/80 monoclonal antibody was generated for a macrophage-specific surface antigen, however F4/80 staining is also observed in other hematopoietic cell populations, thus other immune cell markers should be used in conjunction with F4/80 to distinguish between different populations (1). Though the full extent of F4/80 ligands and biological functions remains elusive, studies have suggested that F4/80 is critical for macrophage-natural killer cell interaction and cytokine release (1,2). Additionally, a study of anterior chamber-associated immune deviation (ACAID) indicated a possible role for F4/80 in the generation of regulatory T cells (1,2).

References

1. Dos Anjos Cassado A. (2017). F4/80 as a Major Macrophage Marker: The Case of the Peritoneum and Spleen. Results and Problems in Cell Differentiation. https://doi.org/10.1007/978-3-319-54090-0_7

2. Hamann, J., Hsiao, C. C., Lee, C. S., Ravichandran, K. S., & Lin, H. H. (2016). Adhesion GPCRs as Modulators of Immune Cell Function. Handbook of Experimental Pharmacology. https://doi.org/10.1007/978-3-319-41523-9_15

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for F4/80 Antibody (NB600-404AF488)(1)

Publication using NB600-404AF488

• Demonbreun AR, Allen MV, Warner JL et al. Enhanced Muscular Dystrophy from Loss of Dysferlin Is Accompanied by Impaired Annexin A6 Translocation after Sarcolemmal Disruption. Am. J. Pathol. 2016-04-09 [PMID: 27070822] (ICC/IF, IHC-Fr, Mouse)
  
  Details:
  This publication used the AF488 conjugated form of this antibody (NB600-404AF488)

FAQs for F4/80 Antibody (NB600-404AF488). (Showing 1 - 3 of 3 FAQ).

1. Are the F4/80/EMR1 antibodies validated in Simple Western?
   • No, F4/80/EMR1 antibodies are not tested in Simple Western at this moment.
2. Does F4/80/EMR1 antibodies come in lyophilized form?
   • Yes, we carry 2 F4/80 antibodies in lyophilized form: MAB5580 and NBP1-06975
3. What is the theoretical molecular weight for F4/80/EMR1 antibodies?
   • The TMW of F4/80/EMR1 antibodies is approximately 160 kDa.

Bioinformatics

• Gene Symbol: Adgre1