ELISA: Mouse FABP2/I-FABP ELISA Kit (Colorimetric) [NBP2-82214] - Samples were spiked with high concentrations of Mouse FABP2/I-FABP and diluted with Reference Standard & Sample Diluent to produce samples with values ...read more
This kit recognizes Mouse FABP2/I-FABP in samples. No significant cross-reactivity or interference between Mouse FABP2/I-FABP and analogues was observed.
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.09 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 6%
Intra-Assay
CV% < 6%
Spike Recovery
average = 99% for serum; average = 96% for EDTA plasma; average = 94% for cell culture media
Storage of components varies. See protocol for specific instructions.
Kit Components
Components
Micro ELISA Plate, dismountable (8 wells x 12 strips); Reference Standard (2 vials); Concentrated (100X) Biotinylated Detection Ab (120 uL); Concentrated (100X) HRP Conjugate (120 uL); Reference Standard & Sample Diluent (20 mL); Biotinylated Detection Ab
Alternate Names for Mouse FABP2/I-FABP ELISA Kit (Colorimetric)
FABP2
FABPI
fatty acid binding protein 2, intestinal
Fatty acid-binding protein 2
fatty acid-binding protein, intestinal
IFABP
I-FABP
I-FABPMGC133132
Intestinal FABP
Intestinal-type fatty acid-binding protein
Background
The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family with nearly twenty identified members. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. They may also be responsible in the modulation of cell growth and proliferation. Intestinal fatty acid-binding protein 2 gene contains four exons and is an abundant cytosolic protein in small intestine epithelial cells. This gene has a polymorphism at codon 54 that identified an alanine-encoding allele and a threonine-encoding allele. Thr-54 protein is associated with increased fat oxidation and insulin resistance.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.