FADD Antibody

Western Blot: FADD Antibody [NBP1-45552] - Analysis of FADD expression with Jurkat cell lysates (Lane 1, 2).

Product Discontinued

FADD Antibody Summary

• Description: This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab24533)
• Immunogen: Synthetic peptide surrounding amino acid 128 of human FADD.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FADD
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot 0.5 - 4 ug/mL
• Theoretical MW: 23 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2), 5 mM EDTA, 50% glycerol, 0.5% BSA
• Preservative: 0.03% Proclin 300
• Concentration: 0.5 mg/ml
• Purity: Affinity purified

Alternate Names for FADD Antibody

• FADD
• Fas (TNFRSF6)-associated via death domain
• FAS-associated death domain protein
• FAS-associating death domain-containing protein
• GIG3
• Growth-inhibiting gene 3 protein
• Mediator of receptor induced toxicity
• mediator of receptor-induced toxicity
• MGC8528
• MORT1
• MORT1Fas-associating protein with death domain
• protein FADD

Background

Fas-associating protein with death domain (FADD) is an adaptor protein involved in the apoptosis induced by Fas and TNF-R1, and mitogen induced cell proliferation (1). FADD consists of death domain (DD) at the C-terminal and death effector domain (DED) at the N-terminal (2). DD interacts with Fas and TNF-R1, which exposes the DED portion on the FADD. Unmasked DED binds to the DED of pro-caspase-8, which activates the cysteine protease cascade (3). The development of autoimmune lymphoproliferative (lpr) diseases has been linked to inactivation of Fas-FADD interaction (4). Also, the deficiency in FADD can inhibit TRAIL-induced cell apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FADD Antibody (NBP1-45552)(1)

Publication using NBP1-45552

• Lindstrom SI, Sigurdardottir S, Zapadka TE et al. Diabetes induces IL-17A-Act1-FADD-dependent retinal endothelial cell death and capillary degeneration J. Diabetes Complicat. 2019-05-29 [PMID: 31239234] (WB, Mouse, Human)

Bioinformatics

• Gene Symbol: FADD
• Entrez:
  • 8772(Human)
• Uniprot:
  • Q13158()