Mouse Fatty Acid Synthase/FASN ELISA Kit (Colorimetric)

ELISA: Mouse Fatty Acid Synthase/FASN ELISA Kit (Colorimetric) [NBP2-82413] - Samples were spiked with high concentrations of Mouse Fatty Acid Synthase/FASN and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Mouse Fatty Acid Synthase/FASN ELISA Kit (Colorimetric) [NBP2-82413] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell supernatant, and other biological fluids
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.09 ng/mL

Mouse Fatty Acid Synthase/FASN ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Mouse Fatty Acid Synthase/FASN in samples. No Significant cross-reactivity or interference between Mouse Fatty Acid Synthase/FASN and analogues was observed.
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.09 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 4.35
• Intra-Assay: CV% < 5.01
• Spike Recovery: 85-109%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: FASN

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse Fatty Acid Synthase/FASN ELISA Kit (Colorimetric)

• EC 2.3.1
• EC 2.3.1.85
• EC 6.3.3.1
• FASN
• FASshort chain dehydrogenase/reductase family 27X, member 1
• Fatty Acid Synthase
• MGC14367
• MGC15706
• OA-519
• SDR27X1

Background

Fatty Acid Synthase (FAS) is a very large enzymatic system involved in de novo lipogenesis (triglyceride production). Specifically, FAS catalyzes the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. It is also one of the accepted markers for insulin resistance and SREBP, LXR activation.

In some cancer cell lines, FAS has been found to be fused with ER-alpha. Therefore, FAS antibodies are useful tools for studies on fatty acid synthesis and research on certian cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: FASN