Fbx32 Antibody

Western Blot: Fbx32 Antibody [NBP1-36955] - Staining of Mouse Skeletal Muscle lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Po, Bv
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Fbx32 Antibody Summary

• Immunogen: Peptide with sequence C-NSKTKTQYFHQEK corresponding to internal region according to NP_478136.1.
• Specificity: This antibody is expected to recognize reported isoform 1 (NP_478136.1).
• Predicted Species: Rat (100%), Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FBXO32
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.3 - 1 ug/mL
• Application Notes: WB: Approx. 40 kDa band observed in human and mouse skeletal muscle lysates (calculated MW of 41.6 kDa band according to human NP_478136.1 and of 41.5 kDa band according to mouse NP_080622.1).
• Theoretical MW: 41.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 28068319).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Fbx32 Antibody

• atrogin 1
• ATROGIN1
• atrogin-1
• F-box only protein 32
• F-box protein 32
• Fbx32
• FLJ32424
• MAFBX
• MAFbxMGC33610
• Muscle atrophy F-box protein

Background

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and contains an F-box domain. This protein is highly expressed during muscle atrophy, whereas mice deficient in this gene were found to be resistant to atrophy. This protein is thus a potential drug target for the treatment of muscle atrophy. Alternative splicing of this gene results in two transcript variants encoding two isoforms of different sizes. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Fbx32 Antibody (NBP1-36955)(3)

Publications using NBP1-36955

• Southwell, N;Primiano, G;Nadkarni, V;Attarwala, N;Beattie, E;Miller, D;Alam, S;Liparulo, I;Shurubor, YI;Valentino, ML;Carelli, V;Servidei, S;Gross, SS;Manfredi, G;Chen, Q;D'Aurelio, M; A coordinated multiorgan metabolic response contributes to human mitochondrial myopathy EMBO molecular medicine 2023-05-24 [PMID: 37222423] (WB, Mouse)
• Zhou H, Liu Y, Zhu R et al. FBXO32 suppresses breast cancer tumorigenesis through targeting KLF4 to proteasomal degradation. Oncogene 2017-06-08 [PMID: 28068319] (WB, Human)
• Hanai J, Cao P, Tanksale P et al. The muscle-specific ubiquitin ligase atrogin-1/MAFbx mediates statin-induced muscle toxicity. J Clin Invest 2007-12-01 [PMID: 17992259]

Bioinformatics

• Gene Symbol: FBXO32
• Entrez:
  • 114907(Human)
  • 67731(Mouse)
  • 171043(Rat)
• Uniprot:
  • Q969P5(Human)