FBXO25 Antibody (2F1) - Azide and BSA Free

Western Blot: FBXO25 Antibody (2F1) [H00026260-M01] - FBXO25 monoclonal antibody (M01), clone 2F1 Analysis of FBXO25 expression in COLO 320 HSR.

Western Blot: FBXO25 Antibody (2F1) [H00026260-M01] - FBXO25 monoclonal antibody (M01), clone 2F1 Western Blot analysis of FBXO25 expression in COLO 320 HSR ( Cat # L020V1 ).

Western Blot: FBXO25 Antibody (2F1) [H00026260-M01] - analysis of FBXO25 expression in transfected 293T cell line by FBXO25 monoclonal antibody (M01), clone 2F1. Lane 1: FBXO25 transfected lysate (43 KDa). Lane 2: Non-transfected lysate.

Sandwich ELISA: FBXO25 Antibody (2F1) [H00026260-M01] - Detection limit for recombinant GST tagged FBXO25 is approximately 3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 2F1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

FBXO25 Antibody (2F1) - Azide and BSA Free Summary

• Immunogen: FBXO25 (AAH50393, 1 a.a. ~ 367 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPFLGQDWRSPGWSWIKTEDGWKRCESCSQKLERENNHCNISHSIILNSEDGEIFNNEEHEYASKKRKKDHFRNDTNTQSFYREKWIYVHKESTKERHGYCTLGEAFNRLDFSSAIQDIRRFNYVVKLLQLIAKSQLTSLSGVAQKNYFNILDKIVQKVLDDHHNPRLIKDLLQDLSSTLCILIRGVGKSVLVGNINIWICRLETILAWQQQLQDLQMTKQVNNGLTLSDLPLHMLNNILYRFSDGWDIITLGQVTPTLYMLSEDRQLWKKLCQYHFAEKQFCRHLILSEKGHIEWKLMYFALQKHYPAKEQYGDTLHFCRHCSILFWKDYHLALLFKDSGHPCTAADPDSCFTPVSPQHFIDLFKF
• Specificity: FBXO25 - F-box protein 25
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: FBXO25
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate, transfected lysate and recombinant protein for western blot. It has also been used for ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for FBXO25 Antibody (2F1) - Azide and BSA Free

• F-box protein 25
• F-box protein Fbx25
• FBX25F-box only protein 25
• MGC20256
• MGC51975

Background

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Three alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FBXO25
• Entrez:
  • 26260(Human)
• OMIM:
  • 609098(Human)
• Uniprot:
  • AAH50393(Human)