Fc epsilon RI Antibody (IE7) - Chimeric - Azide and BSA Free

Flow Cytometry: Fc epsilon RI Antibody (IE7) - Chimeric [NBP3-12052] - U937 cells were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of IE7 (NBP3-12052, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunocytochemistry /Immunofluorescence: Fc epsilon RI Antibody (IE7) - Chimeric - Azide and BSA Free [NBP3-12052] - Immunofluorescence analysis of paraformaldehyde fixed U937 cells immobilized on Shi-fix(TM) cover-slips and stained with NBP3-12052 at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12052, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (NBP2-52638) followed by Alexa Fluor(R) 488 secondary antibody.

• Reactivity: Hu
• Applications: Flow, ICC/IF
• Clone: IE7
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Fc epsilon RI Antibody (IE7) - Chimeric - Azide and BSA Free Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant human protein, N-terminus.
• Specificity: This antibody binds to human Fc epsilon receptor, but does not compete with IgE binding.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: FCER1A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
• Application Notes: IE7 binds to human FCER1A, but does not compete with IgE binding or binding of the antibody IB10. This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Packaging, Storage & Formulations

• Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Fc epsilon RI Antibody (IE7) - Chimeric - Azide and BSA Free

• alpha polypeptide
• Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide
• Fc-epsilon RI-alpha
• FcERI
• high affinity immunoglobulin epsilon receptor alpha-subunit
• high affinity immunoglobulin epsilon receptor subunit alpha
• high-affinity, of mast cells, alpha polypeptide

Background

The allergic reaction is mediated through the IgE high affinity receptor (FceRI). When a multivalent antigen is presented, a redistribution of the monomeric IgE FceRI receptor complexes cause the degranulation of mast cells and basophils, resulting in the subsequent release of factors responsible for the allergic reaction. The FceRI receptor is a tetrameric complex, consisting of a a-chain, b-chain and a dimeric g-chain. While the a-chain is primarily involved with the binding of IgE, the signal is transferred through the g subunit, which contains the immunoreceptor tyrosine activation motifs (ITAM) critical for initiating receptor mediated signal transduction through the Syk and Lyn tyrosine kinases. The FceRI receptor is able to engage and disengage the IgE, providing a versatile model for studying the function of kinase coupled receptors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FCER1A