Fc gamma RII/RIII (CD32/CD16) Antibody [Unconjugated]

Western Blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 1 µg/ml of Goat Anti-Mouse Fc gamma RII/RIII (CD32/CD16) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1460) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Fc gamma RII/RIII (CD32/CD16) at approximately 40-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Fc gamma RII/RIII (CD32/CD16) was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse Fc gamma RII/RIII (CD32/CD16) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1460) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Fc gamma RII/RIII (CD32/CD16) Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Fc gamma RIIB
  Thr40-Arg217
  Accession # P08101
• Specificity: Detects mouse Fc gamma RII/RIII (CD32/CD16) in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross‑reactivity with recombinant human Fc gamma RII is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fc gamma RII/RIII (CD32/CD16) Antibody [Unconjugated]

• CD16;CD16A;CD16-II;FCG3;FCGR3;FCGR3A;FCGRIII;FcGRIIIA;FCR-10;FCRIII;FCRIIIA;IGFR3;IMD20;Low affinity immunoglobulin gamma Fc region receptor III-A
• CD32/CD16
• Fc gamma RII/RIII

Background

Fc gamma RII, also named CD32, is a low-affinity receptor for the Fc region of complexed IgG. Three genes for human Fc gamma RII (A, B and C) and one for mouse (Fc gamma RIIB), encoding type I transmembrane proteins, have been identified. The extracellular domain of mouse Fc gamma RII shares approximately 95% amino acid sequence identity with that of mouse Fc gamma RIII/CD16.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Fc gamma RII/RIII (CD32/CD16) Antibody (AF1460)(23)

Publications using AF1460

• Wohlfeil, SA;Olsavszky, A;Irkens, AL;Häfele, V;Dietsch, B;Straub, N;Goerdt, S;Géraud, C; Deficiency of Stabilin-1 in the Context of Hepatic Melanoma Metastasis Cancers 2024-01-19 [PMID: 38275881] (IHC, Transgenic Mouse, Mouse)
• Antwi, MB;Dumitriu, G;Simón-Santamaria, J;Romano, JS;Li, R;Smedsrød, B;Vik, A;Eskild, W;Sørensen, KK; Liver sinusoidal endothelial cells show reduced scavenger function and downregulation of Fc gamma receptor IIb, yet maintain a preserved fenestration in the Glmpgt/gt mouse model of slowly progressing liver fibrosis PloS one 2023-11-01 [PMID: 37910485] (Western Blot, IHC, Transgenic Mouse, Mouse)
• Jessica Krzistetzko, Cyrill Géraud, Christof Dormann, Anna Riedel, Thomas Leibing Association of Differentially Altered Liver Fibrosis with Deposition of TGFBi in Stabilin-Deficient Mice International Journal of Molecular Sciences 6/30/2023 [PMID: 37446152]
• Jessica Krzistetzko; Cyrill Géraud; Christof Dormann; Anna Riedel; Thomas Leibing Association of Differentially Altered Liver Fibrosis with Deposition of TGFBi in Stabilin-Deficient Mice International Journal of Molecular Sciences 2023-06-30 [PMID: 37446152] (Immunohistochemistry, Transgenic Mouse)
• Qian Xia, Meng Mao, Gaofeng Zhan, Zhenzhao Luo, Yin Zhao, Xing Li SENP3-mediated deSUMOylation of c-Jun facilitates microglia-induced neuroinflammation after cerebral ischemia and reperfusion injury iScience 2023-06-16 [PMID: 37332598] (Western Blot, Mouse)
• Qian Xia, Meng Mao, Gaofeng Zhan, Zhenzhao Luo, Yin Zhao, Xing Li SENP3-mediated deSUMOylation of c-Jun facilitates microglia-induced neuroinflammation after cerebral ischemia and reperfusion injury iScience 6/16/2023 [PMID: 37332598]
• Dietsch, B;Weller, C;Sticht, C;de la Torre, C;Kramer, M;Goerdt, S;G�raud, C;Wohlfeil, SA; Hepatic passaging of NRAS-mutant melanoma influences adhesive properties and metastatic pattern BMC cancer 2023-05-13 [PMID: 37179302] (IHC, Mouse)
• K Zhong, Y Huang, PLM Zilundu, Y Wang, Y Zhou, G Yu, R Fu, SK Chung, Y Tang, X Cheng, L Zhou Motor neuron survival is associated with reduced neuroinflammation and increased autophagy after brachial plexus avulsion injury in aldose reductase-deficient mice Journal of Neuroinflammation, 2022-11-09;19(1):271. 2022-11-09 [PMID: 36352421] (IHC, Mouse)
• M Mao, Q Xia, GF Zhan, QJ Chu, X Li, HK Lian SENP6 induces microglial polarization and neuroinflammation through de-SUMOylation of Annexin-A1 after cerebral ischaemia-reperfusion injury Cell & bioscience, 2022-07-22;12(1):113. 2022-07-22 [PMID: 35869493] (Western Blot, Mouse)
• SA Wohlfeil, V Häfele, B Dietsch, C Weller, C Sticht, AS Jauch, M Winkler, CD Schmid, AL Irkens, A Olsavszky, K Schledzews, PS Reiners-Ko, S Goerdt, C Géraud Angiogenic and molecular diversity determine hepatic melanoma metastasis and response to anti-angiogenic treatment Journal of Translational Medicine, 2022-02-02;20(1):62. 2022-02-02 [PMID: 35109875] (ICC/IF, Mouse)
• Mou KJ, Shen KF, Li YL et al. Adenosine A2A Receptor in Bone Marrow-Derived Cells Mediated Macrophages M2 Polarization via PPAR gamma-P65 Pathway in Chronic Hypoperfusion Situation Frontiers in aging neuroscience 1/3/2022 [PMID: 35046793]
• J Heil, V Olsavszky, K Busch, K Klapproth, C de la Torr, C Sticht, K Sandorski, J Hoffmann, H Schönhaber, J Zierow, M Winkler, CD Schmid, T Staniczek, DE Daniels, J Frayne, G Metzgeroth, D Nowak, S Schneider, M Neumaier, V Weyer, C Groden, HJ Gröne, K Richter, C Mogler, MM Taketo, K Schledzews, C Géraud, S Goerdt, PS Koch Bone marrow sinusoidal endothelium controls terminal erythroid differentiation and reticulocyte maturation Nature Communications, 2021-11-29;12(1):6963. 2021-11-29 [PMID: 34845225] (IHC, Mouse)
• Philipp-Sebastian Koch, Kajetan Sandorski, Joschka Heil, Christian D. Schmid, Sina W. Kürschner, Johannes Hoffmann et al. Imbalanced Activation of Wnt-/ beta -Catenin-Signaling in Liver Endothelium Alters Normal Sinusoidal Differentiation Frontiers in Physiology 9/29/2021 [PMID: 34658910]
• Zhibing Song, Jingjing Feng, Qian Zhang, Shanshan Deng, Dahai Yu, Yuefan Zhang et al. Tanshinone IIA Protects Against Cerebral Ischemia Reperfusion Injury by Regulating Microglial Activation and Polarization via NF-kappa B Pathway Frontiers in Pharmacology 4/19/2021 [PMID: 33953677]
• Y Wang, Y Lin, L Wang, H Zhan, X Luo, Y Zeng, W Wu, X Zhang, F Wang TREM2 ameliorates neuroinflammatory response and cognitive impairment via PI3K/AKT/FoxO3a signaling pathway in Alzheimer's disease mice Aging (Albany NY), 2020-10-16;12(0):. 2020-10-16 [PMID: 33065553] (ICC, IHC, Mouse)
• D Wang, F Liu, L Zhu, P Lin, F Han, X Wang, X Tan, L Lin, Y Xiong FGF21 alleviates neuroinflammation following ischemic stroke by modulating the temporal and spatial dynamics of microglia/macrophages J Neuroinflammation, 2020-08-31;17(1):257. 2020-08-31 [PMID: 32867781] (IF, IHC, Mouse)
• P Ren, J Chen, B Li, M Zhang, B Yang, X Guo, Z Chen, H Cheng, P Wang, S Wang, N Wang, G Zhang, X Wu, D Ma, D Guan, R Zhao Nrf2 Ablation Promotes Alzheimer's Disease-Like Pathology in APP/PS1 Transgenic Mice: The Role of Neuroinflammation and Oxidative Stress Oxid Med Cell Longev, 2020-05-11;2020(0):3050971. 2020-05-11 [PMID: 32454936] (IHC, Transgenic Mouse)
• Baba T, Miyazaki D, Inata K et al. Role of IL-4 in bone marrow driven dysregulated angiogenesis and age-related macular degeneration Elife 5/5/2020 [PMID: 32366355]
  
  Details:
  Citation using the DyLight 550 format of this antibody.
• Thomas Leibing, Cyrill Géraud, Iris Augustin, Michael Boutros, Hellmut G. Augustin, Jürgen G. Okun et al. Angiocrine Wnt signaling controls liver growth and metabolic maturation in mice Hepatology 8/1/2018 [PMID: 29059455]
• Bruno Siqueira Mietto, Antje Kroner, Elizabeth I. Girolami, Eva Santos-Nogueira, Ji Zhang, Samuel David Role of IL-10 in Resolution of Inflammation and Functional Recovery after Peripheral Nerve Injury The Journal of Neuroscience 12/16/2015 [PMID: 26674868]
• Piccioni M, Monari C, Kenno S, Pericolini E, Gabrielli E, Pietrella D, Perito S, Bistoni F, Kozel T, Vecchiarelli A A purified capsular polysaccharide markedly inhibits inflammatory response during endotoxic shock. Infect Immun, 2012-10-22;81(1):90-8. 2012-10-22 [PMID: 23090956] (Western Blot, Mouse)
• Liu F, Chen HY, Huang XR, Chung AC, Zhou L, Fu P, Szalai AJ, Lan HY C-reactive protein promotes diabetic kidney disease in a mouse model of type 1 diabetes. Diabetologia, 2011-07-09;54(10):2713-23. 2011-07-09 [PMID: 21744073] (IHC-P, Mouse)
• Di Valentin E, Crahay C, Garbacki N, Hennuy B, Gueders M, Noel A, Foidart JM, Grooten J, Colige A, Piette J, Cataldo D New asthma biomarkers: lessons from murine models of acute and chronic asthma. Am. J. Physiol. Lung Cell Mol. Physiol., 2008-11-21;296(2):L185-97. 2008-11-21 [PMID: 19028979] (IHC-P, Mouse)

Bioinformatics

• Uniprot:
  • P08101()