Fc gamma RIIB/CD32b Antibody (2E10) - Azide and BSA Free

Western Blot: Fc gamma RIIB/CD32b Antibody (2E10) [H00002213-M01] - Analysis of FCGR2B expression in transfected 293T cell line by FCGR2B monoclonal antibody (M01), clone 2E10.Lane 1: FCGR2B transfected lysate(34 KDa).Lane 2: Non-transfected lysate.

Immunohistochemistry-Paraffin: Fc gamma RIIB/CD32b Antibody (2E10) [H00002213-M01] - Analysis of monoclonal antibody to FCGR2B on formalin-fixed paraffin-embedded human placenta. Antibody concentration 6 ug/ml.

Western Blot: Fc gamma RIIB/CD32b Antibody (2E10) [H00002213-M01] - FCGR2B monoclonal antibody (M01), clone 2E10 Analysis of FCGR2B expression in K-562.

ELISA: Fc gamma RIIB/CD32b Antibody (2E10) [H00002213-M01] - Fc gamma RIIB/CD32b Antibody (2E10) [H00002213-M01] - Detection limit for recombinant GST tagged FCGR2B is 0.3 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 2E10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Fc gamma RIIB/CD32b Antibody (2E10) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: FCGR2B (AAH31992, 45 a.a. ~ 154 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AAPPKAVLKLEPQWINVLQEDSVTLTCRGTHSPESDSIQWFHNGNLIPTHTQPSYRFKANNNDSGEYTCQTGQTSLSDPVHLTVLSEWLVLQTPHLEFQEGETIVVRCHS
• Specificity: FCGR2B - Fc fragment of IgG, low affinity IIb, receptor (CD32)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: FCGR2B
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate, transfected lysate and recombinant protein for WB. It has been used for IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Fc gamma RIIB/CD32b Antibody (2E10) - Azide and BSA Free

• CD32 antigen
• CD32b
• CD32Fc fragment of IgG, low affinity II, receptor for (CD32)
• CDw32
• Fc fragment of IgG, low affinity IIb, receptor (CD32)
• Fc fragment of IgG, low affinity IIb, receptor for (CD32)
• Fc gamma RIIB
• FCG2
• Fc-gamma RII-b
• fc-gamma-RIIb
• FCGR2
• FCGR2B
• FcgRIIB
• FCRIIB
• fcRII-b
• IGFR2
• IgG Fc receptor II-b
• low affinity immunoglobulin gamma Fc region receptor II-b

Background

Low affinity IgG Fc receptor gamma II (Fc-gamma II), also known as CD32, is a member of the immunoreceptor tyrosine-based activating/inhibitory motif (ITAM/ITIM) family. Fc gamma receptors play a critical role in immunity by linking the IgG antibody mediated response with the regulatory functions of the immune system (1). Three classes of Fc gamma receptor exist; Fc gamma RI (CD64, a high affinity receptor), Fc gamma RII, and RIII (CD32 and CD16 respectively, both low affinity receptors). Fc gamma RIIa is an activating receptor, while RIIb1, RIIb2, and RIIb3 are inhibitory receptors generated by alternative splicing (1-2). Fc gamma RIIa is critical for antigen-presenting-cell activation and Fc gamma RIIb downregulates B-cell functions (3). Fc gamma RIIb has also been known to block the inflammatory immune response in mice (4). Once phosphorylated on tyrosine residues, Fc gamma RIIb associates with SHIP to downregulate phagocytosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FCGR2B
• Entrez:
  • 2213(Human)
• OMIM:
  • 604590(Human)
• Uniprot:
  • AAH31992(Human)