FCRN/FCGRT Antibody [Unconjugated]

Western blot shows lysates of mouse liver tissue, mouse small intestine tissue, and mouse kidney tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse FCRN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6775) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for FCRN at approximately 48 to 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Goat Anti-Mouse FCRN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6775, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

• Reactivity: Mu
• Applications: WB, CyTOF-ready, Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

FCRN/FCGRT Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived mouse FCRN
  Ser22-Ser301
  Accession # AAH03786
• Specificity: Detects mouse FCRN in direct ELISAs and Western blots. In direct ELISAs, approximately 6% cross-reactivity with recombinant human FCRN is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FCGRT
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 0.25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FCRN/FCGRT Antibody [Unconjugated]

• alpha-chain
• Fc fragment of IgG, receptor, transporter, alpha
• FCGRT
• FcRn alpha chain
• FCRN
• FCRNimmunoglobulin receptor, intestinal, heavy chain
• IgG Fc fragment receptor transporter alpha chain
• IgG receptor FcRn large subunit p51
• major histocompatibility complex class I-like Fc receptor
• Neonatal Fc receptor
• neonatal Fc-receptor for Ig

Background

FcRn (IgG receptor FcRn large subunit p51/ alpha -chain) is a 44-52 kDa member of the Ig superfamily. In mouse, it is expressed by intestinal epithelium of neonates and fetal yolk sac endoderm during pregnancy. Together with p14/ beta ₂-Microglobulin, FcRn forms an MHC I-like noncovalent heterodimer termed the FcRn complex. In rodent, FcRn transports IgG in milk into the intestine, and IgG from the mother's blood into the mouse via endodermal FcRn linked to a special Fc gamma RIIb macrophage receptor. It is also expressed by select macrophages and DCs, and participates in endosomal-associated antigen presentation to CD4+ T cells. Mature mouse FcRn is a 344 amino acid (aa) type I transmembrane glycoprotein. It contains a 276 aa extracellular region (aa 22-297) that contains an Ig-like domain (aa 201-288). The FcRn complex forms a homodimer when binding IgG. One potential splice variant shows a four aa insertion after Gly109. Over aa 22-301 of the splice variant (GenBank #:AAH03786), mouse FcRn shares 91% and 68% aa identity with rat and human FcRn, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FCRN/FCGRT Antibody (AF6775)(6)

Publications using AF6775

• E Dahlke, Y Anan, LM Klie, AE Hartkopf, F Theilig Megalin Orchestrates FcRn Endocytosis and Trafficking Cells, 2022-12-22;12(1):. 2022-12-22 [PMID: 36611847] (Western Blot, Mouse, Opossum)
• ND Arnold, JA Pickworth, LE West, S Dawson, JA Carvalho, H Casbolt, AT Braithwait, J Iremonger, L Renshall, V Germaschew, M McCourt, P Bland-Ward, H Kowash, AG Hameed, AMK Rothman, MG Frid, AA Roger Thom, HR Evans, M Southwood, NW Morrell, DC Crossman, MKB Whyte, KR Stenmark, CM Newman, DG Kiely, SE Francis, A Lawrie A therapeutic antibody targeting osteoprotegerin attenuates severe experimental pulmonary arterial hypertension Nat Commun, 2019-11-15;10(1):5183. 2019-11-15 [PMID: 31729368] (Neutralization Control, Mouse)
• S Ono, G Egawa, T Nomura, A Kitoh, T Dainichi, A Otsuka, S Nakajima, M Amagai, F Matsumoto, M Yamamoto, Y Kubota, T Takai, T Honda, K Kabashima Abl family tyrosine kinases govern IgG extravasation in the skin in a murine pemphigus model Nat Commun, 2019-09-30;10(1):4432. 2019-09-30 [PMID: 31570755] (Flow Cytometry, Mouse)
• DJ Royer, MM Carr, HR Gurung, WP Halford, DJJ Carr The Neonatal Fc Receptor and Complement Fixation Facilitate Prophylactic Vaccine-Mediated Humoral Protection against Viral Infection in the Ocular Mucosa J. Immunol., 2017-07-31;0(0):. 2017-07-31 [PMID: 28760885] (IHC, Western Blot, Mouse)
• S Latvala, B Jacobsen, MB Otteneder, A Herrmann, S Kronenberg Distribution of FcRn Across Species and Tissues J. Histochem. Cytochem., 2017-04-12;0(0):2215541770509. 2017-04-12 [PMID: 28402755] (IHC-P, Human, Mouse, Primate, Rat)
• Deletion of Wiskott-Aldrich syndrome protein triggers Rac2 activity and increased cross-presentation by dendritic cells Nat Commun, 2016-07-18;7(0):12175. 2016-07-18 [PMID: 27425374] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: FCGRT
• Uniprot:
  • AAH03786(Mouse)