FEN-1 Antibody

Western Blot: FEN-1 Antibody [H00002237-D01P] - Analysis of FEN1 expression in mouse lung.

Western Blot: FEN-1 Antibody [H00002237-D01P] - Analysis of FEN1 expression in MCF-7.

Western Blot: FEN-1 Antibody [H00002237-D01P] - Analysis of FEN1 expression in transfected 293T cell line by FEN1 polyclonal antibody.Lane 1: FEN1 transfected lysate(42.60 KDa).Lane 2: Non-transfected lysate.

Product Discontinued

FEN-1 Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: FEN1 (NP_004102.1, 1 a.a. - 380 a.a.) full-length human protein. MGIQGLAKLIADVAPSAIRENDIKSYFGRKVAIDASMSIYQFLIAVRQGGDVLQNEEGETTSHLMGMFYRTIRMMENGIKPVYVFDGKPPQLKSGELAKRSERRAEAEKQLQQAQAAGAEQEVEKFTKRLVKVTKQHNDECKHLLSLMGIPYLDAPSEAEASCAALVKAGKVYAAATEDMDCLTFGSPVLMRHLTASEAKKLPIQEFHLSRILQELGLNQEQFVDLCILLGSDYCESIRGIGPKRAVDLIQKHKSIEEIVRRLDPNKYPVPENWLHKEAHQLFLEPEVLDPESVELKWSEPNEEELIKFMCGEKQFSEERIRSGVKRLSKSRQGSTQGRLDDFFKVTGSLSSAKRKEPEPKGSTKKKAKTGAAGKFKRGK
• Specificity: FEN1 - flap structure-specific endonuclease 1,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FEN1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for FEN-1 Antibody

• EC 3.1
• FEN1
• FEN-1
• FEN-1hFEN-1
• flap structure-specific endonuclease 1DNase IV
• Maturation factor 1
• maturation factor-1
• MF1flap endonuclease 1
• RAD2

Background

The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FEN1
• Entrez:
  • 2237(Human)
• Uniprot:
  • NP_004102.1(Human)