Ferroportin/SLC40A1 Antibody (8G10NB) - BSA Free

Western Blot: Ferroportin/SLC40A1 Antibody (8G10NB) [NBP2-45356] - Analysis using Ferroportin/SLC40A1 Antibody (8G10NB) in (A) recombinant human Ferroportin protein at 0.5 ug/ml (Molecular weight: 62.5 KDa) and (B) NCCIT lysate at 2 ug/ml.

Flow Cytometry: Ferroportin/SLC40A1 Antibody (8G10NB) [NBP2-45356] - An intracellular stain was performed on HepG cells with NBP2-45356F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.

Immunohistochemistry-Paraffin: Ferroportin/SLC40A1 Antibody (8G10NB) [NBP2-45356] - Analysis of a FFPE human spleen section using mouse monoclonal Ferroportin/SLC40A1 Antibody (8G10NB) at 5 ug/ml concentration with HRP-DAB detection followed by hematoxylin counterstaining. The antibody generated membrane cytoplasmic staining in several cells and the signal was very strong in the splenic macrophages.

Flow Cytometry: Ferroportin/SLC40A1 Antibody (8G10NB) [NBP2-45356] - Detection of Ferroportin/SLC40A1 in HEK293 human cell line transfected with human Ferroportin/SLC40A1. HEK293 human embryonic kidney cell line transfected with human Ferroportin/SLC40A1 (filled histogram) or irrelevant transfectants (open histogram) were stained with Mouse Ferroportin/SLC40A1 Antibody (8G10NB) followed by APC-conjugated Anti-mouse IgG Secondary Antibody (R&D Systems Catalog # F0101B).

Flow (Intracellular): Ferroportin/SLC40A1 Antibody (8G10NB) [NBP2-45356] - An intracellular stain was performed on HepG2 cells with NBP2-45356AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Flow Cytometry: Ferroportin/SLC40A1 Antibody (8G10NB) [NBP2-45356] - An intracellular stain was performed on HepG2 cells with NBP2-45356AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Product Discontinued

Ferroportin/SLC40A1 Antibody (8G10NB) - BSA Free Summary

• Immunogen: This Ferroportin/SLC40A1 Antibody (8G10NB) was developed against partial recombinant human Ferroportin protein (between amino acids 150-350) [Uniprot: Q9NP59].
• Localization: Membrane, Cytoplasm
• Predicted Species: Mouse (94%), Rat (93%). Backed by our 100% Guarantee.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLC40A1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 2-4 ug/ml
• Theoretical MW: 62.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Ferroportin/SLC40A1 Antibody (8G10NB) - BSA Free

• Ferroportin
• Ferroportin-1
• FPN1
• FPN1IREG1ferroportin 1
• HFE4
• HFE4ferroportin-1
• IREG1
• iron regulated gene 1
• Iron-regulated transporter 1
• member 3
• MST079
• MSTP079
• MTP1
• putative ferroportin 1 variant IIIB
• SLC11A3
• SLC11A3iron regulated gene 1
• SLC40A1
• solute carrier family 11 (proton-coupled divalent metal ion transporters)
• solute carrier family 11 (proton-coupled divalent metal ion transporters), member 3
• solute carrier family 40 (iron-regulated transporter), member 1
• solute carrier family 40 member 1

Background

Ferroportin is a 12-transmembrane domain protein, belonging to the major facilitator superfamily of transporters of small molecules, that is localized to the plasma membrane. Human Ferroportin has a theoretical molecular weight of 62.5 kDa. Ferroportin (FPN1 or SLC40A1) functions as an iron-regulated transporter (highly expressed in placenta, intestine, muscle, spleen, macrophages etc.) and is the receptor for the iron-regulatory hormone, hepcidin. In iron metabolism, FPN1 plays a key role in intestinal iron absorption as well as cellular iron release and mediates iron absorption in the presence of ferroxidases, hephaestin (HP) and/or ceruloplasmin (CP). FPN1 is implicated in iron export from duodenal epithelial cells and in the transfer of iron between maternal and fetal circulation. FPN1 transports iron in the ferrous form whereas plasma transferrin only binds iron's ferric form. Ferroxidases are key players in oxidizing iron transported by FPN1 and without the activity of ferroxidases, FPN1 is internalized followed by degradation. While other cell types utilize the circulating or GPI-linked multicopper ferroxidase CP for FPN1, intestinal cells utilize a membrane-bound HP, a paralog of CP that also show interaction with FPN1 (1).

FPN1 regulation is dependent on the cell type and involves transcriptional, posttranscriptional, and posttranslational mechanisms including hepcidin-mediated endocytosis and proteolysis. Hepcidin controls the concentration of FPN1 in the membrane, with hepcidin deficiency resulting in iron overload (high iron) and hepcidin excess leading to iron restriction and anemia (2). Ferroportin disease or hemochromatosis type 4 (HFE4) is associated with distinct FPN1 variants with either reduced FPN1 cell surface expression/iron export capacity or hepcidin resistance and iron overload (3, 4).

References

1. De Domenico I, Ward DM, Kaplan J. (2011) Hepcidin and ferroportin: the new players in iron metabolism. Semin Liver Dis. 31(3):272-9. PMID: 21901657

2. Drakesmith H, Nemeth E, Ganz T. (2015) Ironing out Ferroportin. Cell Metab. 22(5):777-87. PMID: 26437604

3. Pietrangelo A. (2017) Ferroportin disease: pathogenesis, diagnosis and treatment. Haematologica. 102(12):1972-1984. PMID: 29101207

4. Vlasveld LT, Janssen R, Bardou-Jacquet E, Venselaar H, Hamdi-Roze H, Drakesmith H, Swinkels DW. (2019) Twenty Years of Ferroportin Disease: A Review or An Update of Published Clinical, Biochemical, Molecular, and Functional Features. Pharmaceuticals (Basel). 12(3). pii: E132. PMID: 31505869

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ferroportin/SLC40A1 Antibody (NBP2-45356)(2)

Publications using NBP2-45356

• Brown CW, Amante JJ, Chhoy P et al. Prominin2 Drives Ferroptosis Resistance by Stimulating Iron Export Dev Cell. 2019-12-02 [PMID: 31735663]
  
  Details:
  Citation used the Azide Free format of this antibody.
• Trujillo-Alonso V, Pratt EC, Zong H et al. FDA-approved ferumoxytol displays anti-leukaemia efficacy against cells with low ferroportin levels Nat Nanotechnol 2019-03-25 [PMID: 30911166] (FLOW, Human)

FAQs for Ferroportin/SLC40A1 Antibody (NBP2-45356). (Showing 1 - 1 of 1 FAQs).

1. Hi,I am looking for the fixing and permeabilization buffers for flow cytometry for NBP2 45356 products
   • Here is a link to our Flow protocol which lists our recommended buffers

Bioinformatics

• Gene Symbol: SLC40A1
• Entrez:
  • 30061(Human)
• Uniprot:
  • Q9NP59()