Human FGF basic/FGF2/bFGF ELISA Kit (Colorimetric)

ELISA: Human FGF basic/FGF2 ELISA Kit [KA0527] - The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.

Product Discontinued

Human FGF basic/FGF2/bFGF ELISA Kit (Colorimetric) Summary

• Description: Quality control test: Standard curve. FGF2 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human FGF2.
• Standard Curve Range: 78 to 8000 pg/mL
• Sensitivity: 78 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 4.69%
• Intra-Assay: CV% < 6.39%
• Spike Recovery: Serum: 94.00%, Plasma: 102.00%, Cell Culture Supernatant: 108.00%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: FGF2

Applications/Dilutions

• Dilutions:
  • ELISA
  • Quantification
• Application Notes: Useful for quantification.

Packaging, Storage & Formulations

• Storage: Store at 4C.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human FGF basic/FGF2/bFGF ELISA Kit (Colorimetric)

• basic fibroblast growth factor bFGF
• Basic fibroblast growth factor
• bFGF
• FGF basic
• FGF2
• FGF-2
• FGFBprostatropin
• fibroblast growth factor 2 (basic)
• HBGF-2
• heparin-binding growth factor 2
• Prostatropin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for FGF basic/FGF2/bFGF ELISA Kit (KA0527)(3)

Publications using KA0527

• Smith JM, Sporn MB, Roberts AB et al. Human transforming growth factor-alpha causes precocious eyelid opening in newborn mice. Nature. 1985-06-06 [PMID: 3873618]
• Tam JP. Physiological effects of transforming growth factor in the newborn mouse. Science. 1985-08-16 [PMID: 3860952]
• Barrandon Y, Green H. Cell migration is essential for sustained growth of keratinocyte colonies: the roles of transforming growth factor-alpha and epidermal growth factor. Cell. 1987-09-25 [PMID: 3497724]

FAQs for FGF basic/FGF2/bFGF ELISA Kit (KA0527). (Showing 1 - 1 of 1 FAQs).

1. I am looking for an ovine antibody for bFGF and PDGF-AB and ELISA Kits for both. It is for a research work on meniscus healing in sheep. Do you have anything suitable? How is the homology with sheep? Is it ever tested and/or published in papers?
   • Unfortunately, most of the products you are interested in have not yet been tested in sheep, but this should not be a problem, due to sequence homology, and our Innovators Reward Program. In terms of an antibody for bFGF, also called FGF-2, we have several antibodies that are validated to detect the bovine protein, which is almost identical to that from sheep (99% homology). We only have FGF2 ELISA kits that have been tested with human or mouse. Mouse FGF-2 has 94% homology with sheep; human has 98% homology for most of its sequence, but also has an additional stretch of amino acids that is not present in the sheep protein. I therefore think that the mouse kit would be most suitable, just in case the human kit targets the region of the human protein not present in mouse. We sell antibodies to PDGF-A or PDGF-B rather than PDGF-AB. We don't have any PDGF-A antibodies that have been tested in sheep but there are several options you may find of interest. There isn't a sheep PDGFA sequence in UniProt, so I can't comment on how similar it is to the PDGFA from other species. Nevertheless, our NBP1-52533 PDGFB antibody has been shown to work in sheep. We sell two PDGF-AB ELISA kits , but these are for human and rat. I'm not sure how well these would work with sheep. Note that if you test a product with a species (or application) that it has not yet been validated in, you are eligible for our Innovator's Reward: Novus would provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal or lesser value. You would email innovators@novusbio.com to apply for your award, and we

Bioinformatics

• Gene Symbol: FGF2