FGFR1 Antibody (MM0277-6F10) - Azide and BSA Free

Product Discontinued

FGFR1 Antibody (MM0277-6F10) - Azide and BSA Free Summary

• Immunogen: Human recombinant FGFR1 (extracellular domains of mixed isoforms)
• Specificity: Detect human FGF-R1 IIIb isoform only
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FGFR1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • Western Blot 1:500-1:1000

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:25024372).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein G purified
• Reconstitution Instructions: Reconstitute with sterilized PBS to a final concentration of 0.5 mg/ml.

Alternate Names for FGFR1 Antibody (MM0277-6F10) - Azide and BSA Free

• basic fibroblast growth factor receptor 1
• BFGFR
• bFGF-R-1
• CD331 antigen
• CD331
• CEK
• EC 2.7.10
• EC 2.7.10.1
• FGF R1
• FGFBR
• FGFR1
• FGFR-1
• fibroblast growth factor receptor 1
• FLGH3
• FLJ99988
• Flt-2
• FLT2H4
• Fms-like tyrosine kinase 2
• fms-related tyrosine kinase 2
• H2
• H5
• HBGFR
• heparin-binding growth factor receptor
• hydroxyaryl-protein kinase
• KAL 2
• KAL2
• N-SAM
• OGD
• Proto-oncogene c-Fgr
• soluble FGFR1 variant 1
• soluble FGFR1 variant 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FGFR1 Antibody (NBP2-12308)(1)

Publication using NBP2-12308

• Liang Q, Zhong L et al. FGF21 maintains glucose homeostasis by mediating the cross talk between liver and brain during prolonged fasting. Diabetes 2014-01-12 [PMID: 25024372] (WB, Mouse)

FAQs for FGFR1 Antibody (NBP2-12308). (Showing 1 - 3 of 3 FAQ).

1. I would like to inquire whether you have samples of the following antibodies: NBP1-61338, NB600-1287, NBP1-19481, NBP1-19864; The datasheets for these antibodies show different molecular weights of the detected proteins so its no altogether clear to me which one detects the real protein. To sort this out, we would be using FGFR1-deficient cells and would very much appreciate if you could supply us with small aliquots of these antibodies.
   • FGFR1 has multiple isoforms and is subject to multiple PTM's. The molecular weight can vary greatly depending on experimental conditions and samples used. We fully guarantee all of our products for the listed applications and species. If you cannot get a product to work in an application or species stated on our datasheet, our technical service team will troubleshoot with you to get it to work. If a product still does not work after troubleshooting, you can receive a free of charge replacement product or a full refund. Due to our 100% guarantee, we do not offer free of charge samples of our products. If a smaller sample size is available, it will be listed on our product page.
2. I am working in Multiple sclerosis group and mainly working with mice model and cell culture study. I need FGFR1 antibody against Mice for FACS analysis. If it is available can I get the sample to check with my cells? And any Oligodendrocyte markers against mice for FACS too.
   • We do have an antibody that has been tested in FLOW, NB600-1287, however it has not been tested in mouse. If you would like to test this antibody in mouse samples we have an Innovators Reward Program where we reward you for trying our antibody in species and applications that have not been previously tested.
3. What is the recommended concentration for Blocking/Neutralizing?
   • When performing blocking/neutralizing experiments with another anti-human FGFR1 antibody, please use the molar ration of 50 - 200 molar in excess; for example: If NBP2-12308 will be used to block/neutralize antibody A in WB and it need 1 ug/ml of A for the WB experiments, then please do this calculations: 1), Antibody A's molar concentration: 1 ug divides by 160 g/mole in 1 ml [we regularly use 160 kDa as the MW for one IgG molecule], and say it equals to the value of N. 2), Then the amount that is required for using NBP2-12308 in blocking/neutralizing will be 50 - 200 times N. 3) And use the above result in #2 (the number) for calculate how many ug of NBP2-12308 will be needed (again use 160 kDa per IgG to calculate) to add to the reaction solution for blocking/neutralizing.

Bioinformatics

• Gene Symbol: FGFR1
• Uniprot:
  • NP_056934()