Fibroblast Activation Protein alpha/FAP Antibody [Biotin]

• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Biotin
• Concentration: LYOPH

Fibroblast Activation Protein alpha/FAP Antibody [Biotin] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Fibroblast Activation Protein alpha /FAP
  Leu26-Asp760
  Accession # Q12884
• Specificity: Detects human Fibroblast Activation Protein alpha /FAP in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human DPPIV is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: FAP
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Application Notes: ELISA Capture: Human Fibroblast Activation Protein alpha/FAP Antibody (Catalog # MAB3715)
  ELISA Detection: Human Fibroblast Activation Protein alpha/FAP Biotinylated Antibody (Catalog # BAF3715)
  Standard:Recombinant Human Fibroblast Activation Protein alpha/FAP (Catalog # 3715-SE)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

FAP (also known as seprase) is a Type II transmembrane serine protease that is structurally related to dipeptidyl peptidase IV (1). FAP has substrate specificity similar to dipeptidyl peptidase IV, which is specific for N-terminal Xaa-Pro sequences, but FAP is also an endopeptidase able to degrade gelatin and Type I collagen (2). The enzymatically active form of FAP is a dimer (3). FAP has a restricted tissue distribution. It is not detectable in normal tissues or resting fibroblasts, but is highly expressed on reactive stromal fibroblasts in epithelial cancers (4), in granulation tissue during wound healing, and in bone and soft tissue sarcomas (5). Because of its expression patterns and enzymatic activities, FAP is believed to play roles in tumor invasion, tissue remodeling, and wound repair.

1. Scanlan, M.J. et al. (1994) Proc. Natl. Acad. Sci. USA 91:5657.
2. Park, J.E. et al. (1999) J. Biol. Chem. 274:36505.
3. Pineiro-Sanchez, M.L. et al. (1997) J. Biol. Chem. 272:7595.
4. Garin-Chesa, P. et al. (1990) Proc. Natl. Acad. Sci. USA 87:7235.
5. Rettig, W.J. et al. (1988) Proc. Natl. Acad. Sci. USA 85:3110.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FAP